Ingredient Analysis

Identification of Panax ginseng, Panax quinquefolius and Panax notoginseng by EST-SSR markers*

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  • 1. College of Medical Technology, Beihua University, Jilin 132013, China;
    2. National Ginseng Products Quality Supervision Inspection Center, Yanbian 133000, China;
    3. Jilin Yisheng Hanshen Biotechnology Co., Ltd, Baishan 134521, China

Received date: 2021-01-22

  Online published: 2024-06-26

Abstract

Objective: Using the simple sequence repeat (SSR) markers to identify Panax ginseng, Panax quinquefolium and Panax notoginseng. Methods: The EST database of NCBI was pre-processed by using EGassembler. SSRIT website was used to identify SSR loci and Primer3 was used to design specific primers. The universality and polymorphism of EST-SSR primers were screened and verified by non-denatured polyacrylamide gel electrophoresis and capillary electrophoresis. These SSR primers were used to construct standard fingerprints and identify the raw materials of ginseng products from the market. Results: Through bioinformatics methods, seven pairs of EST-SSR markers were screened for identification of Panax ginseng, Panax quinquefolium and Panax notoginseng. The combination of primers P2 and Q11 showed the highest positive rate in the detection of commercial products. Conclusion: SSR molecular markers are repeatable and accurate, which can be used as reliable molecular markers for identification of Panax species.

Cite this article

FAN Xin-yuan . Identification of Panax ginseng, Panax quinquefolius and Panax notoginseng by EST-SSR markers*[J]. Chinese Journal of Pharmaceutical Analysis, 2022 , 42(3) : 394 -401 . DOI: 10.16155/j.0254-1793.2022.03.05

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