Objective: To evaluate quality of Ormosia hosiei Hemsl. Et Wils.(O. hosiei), 12 flavonoids were determined by ultra-high performance liquid chromatography tandem mass spectrometry method. Methods: Chromatographic separation was performed on a Waters CORTECS UPLC C18 column (2.1 mm×100 mm, 1.6 μm) with mobile phase consisting of 0.1% formic acid aqueous solution(A) and acetonitrile(B)in gradient elution (0-0.5 min,5%B;0.5-1.5 min,5%B→10%B;1.5-2.5 min,10%B→15%B;2.5-4.5 min,15%B→20%B;4.5-7.5 min,20%B;7.5-8.0 min,20%B→23%B;8.0-10.85 min,23%B;10.85-11.0 min,23%B→26%B;11.0-13.8 min,26%B→40%B;13.8-13.9 min,40%B→5%B;13.9-15.5 min,5%B). The flow rate was set at 0.2 mL·min-1 and the column temperature was 45 ℃. MS monitoring was performed by electrospray ionization in positive ionization mode, quantification was performed using multiple reaction monitoring mode, and SPSS software was employed to perform the hierarchical cluster analysis for the content determination results. Results: The calibration curves for 12 compounds had good linearity (r2≥0.998 6) in their linear ranges. The average recoveries were in the range of 94.8%-100.8% (RSD≤4.70%), the RSDs of precision,repeatability, and stability were all below 4.7%. Cluster analysis classified 16 batechesof O.hosiei into 2 categories with the leaves as categoryⅠand the twigs (S9-S16) as category Ⅱ. Great differencesexisted in the contents of componentsin different parts of O. hosiei. Conclusion: The established method is simple, rapid, sensitive and specific, and can be used for quality evaluation of O. hosiei.
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