Objective: To establish an UPLC fingerprint of Dahuang Zhechong pills, and evaluate the effect of 60Co-γ radiation sterilization on the fingerprint by studying the changes of UPLC fingerprint before and after irradiation by 60Co-γ ray. Methods: A Waters Acquity UPLC HSS T3 column (100 mm×2.1 mm, 1.8 μm) was used, and the mobile phase was consisted of acetonitrile-0.2% formic acid solution at a flow rate of 0.3 mL·min-1. The detection wavelengths were 210 nm, 230 nm, 254 nm and 270 nm and the column temperature was 35 ℃. Dahuang Zhechong Pills was irradiated by 60Co-γ ray with the dosage of 0, 3, 5, 7 and 9 KGy, respectively. Similarity analysis and multivariate statistical analysis were used to distinguish Dahuang Zhechong Pills from different sources, and to analyze the effects of different doses of 60Co-γ on the chemical constituents of Dahuang Zhechong Pills. Results: Through methodological investigation and the determination of 14 batches of samples, the UPLC fingerprint of Dahuang Zhechong Pills was established and 26 common peaks were confirmed. The similarities of samples to the reference fingerprint were 0.723-0.990, and 9 components were found to cause the difference of Dahuang Zhechong pills. When the irradiation dose of 60Co-γ is high, the 8 components in the Dahuang Zhechong Pills might be affected. Conclusion: The established method for UPLC fingerprint of Dahuang Zhechong pills is accurate, reliable and reproducible. It can distinguish the different sources of Dahuang Zhechong pills, and analyze the influence of 60Co-γ irradiation sterilization on the quality of Dahuang Zhechong pills.
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