Objective: To establish the HPLC characteristic chromatogram of Tibetan herbal medicines Biebersteinia heterostemon and determination the contents of umbelliferone, isoquercitrin, luteolin and apigenin. Methods: Taking umbelliferide, isodendrobioside, luteolin and apigenin astarget compounds, HPLC analysis was performed on a CAPCELL PAK-C18 column(250 mm×4.6 mm, 5 μm) with mobile phase consistingof methanol (A)-0.1% phosphoric acid aqueous solution(B)(0-25 min,16%A→40%A,25-30 min, 40%A, 30-45 min, 40%A→62%A, 45-51 min, 62%A→80%A, 51-55 min, 80%A, 55-56 min, 80%A→16%A, 56-60 min,16%A) at a flow rate of 1 mL·min-1. The column temperature was 30 ℃, and the detection wavelength was 320 nm. Using TCM chromatographic fingerprint similarity evaluation software(2012.130723 version) established the characteristicchromatogram and determined the contents of four components in nine batches of Biebersteinia heterostemon. Results: There were altogether 27 common peaks in the HPLC characteristic chromatogram, and the similaritiesof 9 batches samples ranged from 0.612 to 0.985. The contents of umbelliferone, isoquercitrin, luteolin and apigenin were 63.986-580.347 μg·g-1, 55.361-259.068 μg·g-1, 16.489-86.714 μg·g-1 and 20.231-37.108 μg·g-1, respectively. Conclusion: This method is stable and feasible, and can be used for the quality control of Tibetan herbal medicines Biebersteinia heterostemon.
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