Standard Deliberation

Determination of five triterpene glycosides in Fufang Danshen tablets by HPLC coupled with solid phase extraction

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  • 1. Nano Chrom Technologies (Suzhou) Co., Ltd., Suzhou 215123, China;
    2. Suzhou Nanomicro Technology Co., Ltd., Suzhou 215123, China

Received date: 2020-11-08

  Online published: 2024-06-26

Abstract

Objective: To establish a rapid and accurate assay method using solid phase extraction (SPE) and HPLC for simultaneously determining five triterpene glycosides in Fufang Danshen tablets. Methods: The sample was extracted with 70% methanol and purified with polystyrene coated silica bonding C18 SPE cartridge. A gradient elution with acetonitrile and 0.1% phosphoric acid was performed on ChromCore 300 C18 (100 mm×3.0 mm, 3 μm) at wavelength of 203 nm. Results: The target compounds in Fufang Danshen tablets, i.e. notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ginsenoside Rd were simultaneously detected without interference within an analytical time of 20 min. All calibration curves showed good linear regression (r>0.999 9) within ranges of 5.0-100.0, 20.0-400.0, 5.0-100.0, 20.0-400.0, 20.0-400.0 and 20.0-400.0 μg·mL-1, respectively. The established method showed good repeatability (RSD≤1.0%) and good recovery (ranging from 94.4% to 98.2%, RSD≤1.4%, n=6). Conclusion: The method is reliable for the quantification of four triterpene glycosides in Fufang Danshen tablets.

Cite this article

ZHANG Yong . Determination of five triterpene glycosides in Fufang Danshen tablets by HPLC coupled with solid phase extraction[J]. Chinese Journal of Pharmaceutical Analysis, 2022 , 42(3) : 510 -517 . DOI: 10.16155/j.0254-1793.2022.03.19

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