Objective: To establish an LC-MS/MS method for simultaneous determination of 13 components (geniposide, albiflorin, paeoniflorin, forsythoside A, liquiritin, baicalin, forsythin, oroxylin A-7-O-β-D-glucuronide, wogonoside, baicalein, wogonin, rhein and glycyrrhizic acid) in Xiao’er Chiqiao Qingre granules. Methods: The separation was performed on a Poroshell 120 SB-C18 column(100 mm×3.0 mm, 2.7 μm) by gradient elution, with mobile phase consisting of 0.1% formic acid aqueous and methanol-acetonitrile(1∶1) at a flow rate of 0.45 mL·min-1and the column temperature of 40 ℃. The MS analysis was performed on a triple quadrupole mass spectrometer with an electrospray ionization(ESI) source operated in negative ion mode and multiple reaction monitoring(MRM). Results: The method had a good linearity in the ranges of 9-900 ng·mL-1 for geniposide, 1-100 ng·mL-1 for albiflorin, 12-1 200 ng·mL-1 for paeoniflorin, 12-1 800 ng·mL-1 for forsythoside A, 1-100 ng·mL-1 for liquiritin, 8-800 ng·mL-1 for baicalin, 5-500 ng·mL-1 for forsythin, 5-500 ng·mL-1 for oroxylin A-7-O-β-D-glucuronide, 5-500 ng·mL-1 for wogonoside, 2-200 ng·mL-1 for baicalein, 0.4-40 ng·mL-1 for wogonin, 0.4-40 ng·mL-1 for rhein and 5-500 ng·mL-1 for glycyrrhizic acid(r>0.998 5). The average recoveries(n=6) of them were 97.0%-110.5% with the RSDs of 3.2%-9.6%. The contents of 13 components in 5 batches of Xiao’er Chiqiao Qingre granules were 4.933-5.800 mg·g-1 of geniposide, 0.104-0.123 mg·g-1 of albiflorin, 3.140-4.159 mg·g-1 of paeoniflorin, 17.40-21.05 mg·g-1 of forsythoside A, 0.354-0.425 mg·g-1 of liquiritin, 4.991-6.422 mg·g-1 of baicalin, 1.446-1.906 mg·g-1 of forsythin, 0.441-0.534 mg·g-1 of oroxylin A-7-O-β-D-glucuronide, 0.925-1.138 mg·g-1 of wogonoside, 0.129-0.151 mg·g-1 of baicalein, 0.084-0.106 mg·g-1 of wogonin, 0.051-0.063 mg·g-1 of rhein and 0.964-1.242 mg·g-1 of glycyrrhizic acid. Conclusion: The method is specific, sensitive, effective and reliable, and can be used for quality control of Xiao’er Chiqiao Qingre granules.
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