Objective: To set up a simultaneous determination method of 13 components in Yinqiao Huopu Tuire mixture (Xinguan 2) including neochlorogenic acid, caffeic acid, chlorogenic acid, amygdalin, forsythoside B, forsythoside A, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, forsythin, arctiin, magnolol and honokiol by HPLC-DAD wavelength switching method. Methods: Agilent ZORBAX SB-C18 column (250 mm×4.6 mm, 5 μm) was adopted. The mobile phase composed of methanol, acetonitrile, purified water and phosphoric acid with gradient elution. The flow rate was 1.0 mL·min-1; the column temperature was 40 ℃, and the detection wavelength were 327 nm(0-42 min, neochlorogenic acid), 207 nm (42-60 min, caffeic acid, chlorogenic acid and amygdalin), 327 nm (60-113.5 min, forsythoside A and forsythoside B, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C) and 228 nm(113.5-144 min, forsythin and arctiin) and 210 nm (144-160 min, magnolol and honokiol). Results: The separation of 13 components was good, and there was a good linear relationship between the peak area and the injection quantity of 13 components in the ranges of 0.013 7-0.274 μg·mL-1 (r=0.999 9), 0.009 24-0.185 μg·mL-1 (r=0.999 9), 0.122-2.444 μg·mL-1(r=1.000), 0.019 8-0.396 0 μg·mL-1 (r=0.999 9), 0.012 8-0.257 μg·mL-1(r=1.000), 0.023 1-0.461 μg·mL-1 (r=0.999 9), 0.031 4-0.628 μg·mL-1(r=0.999 9), 0.300-5.992 μg·mL-1 (r=0.999 9), 0.022 0-0.441 μg·mL-1 (r=0.999 9), 0.009 66-0.193 2 μg·mL-1 (r=0.999 9), 0.043 8-0.876 μg·mL-1 (r=0.999 9), 1.452×10-3-0.029 0 μg·mL-1 (r=0.999 8) and 0.660×10-3-0.013 2 μg·mL-1(r=0.999 8), respectively, and the recovery rates were 100.1(RSD=0.78%), 98.9%(RSD=1.1%), 99.8%(RSD=0.72%), 99.7%(RSD=0.61%), 99.9%(RSD=0.38%), 99.6%(RSD=0.17%), 100.3%(RSD=0.87%), 99.6%(RSD=0.21%), 99.9%(RSD=0.64%), 99.6%(RSD=0.91%), 100.0%(RSD=0.55%), 99.3%(RSD=0.21%), 98.9%(RSD=0.67%), respectively. The content ranges of neochlorogenic acid, caffeic acid, chlorogenic acid, amygdalin, forsythoside B, forsythoside A, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, forsythin, arctiin, magnolol and honokiol in three batches of samples were 0.378-0.399 mg·mL-1, 0.080 8-0.085 1 mg·mL-1, 2.193-2.302 mg·mL-1, 0.815-0.878 mg·mL-1, 0.235-0.257 mg·mL-1, 1.877-1.978 mg·mL-1, 0.315 -0.334 mg·mL-1, 0.479-0.493 mg·mL-1, 0.653-0.667 mg·mL-1, 0.223-0.234 mg·mL-1, 1.198-1.258 mg·mL-1, 0.068 1-0.072 1 mg·mL-1 and 0.092 0-0.094 0 mg·mL-1, respectively. Conclusion: The quantitative analysis method established by HPLC-DAD wavelength switching method is proved to meet the requirements, and 13 effective components in Yinqiao Huopu Tuire mixture(Xinguan 2) can be detected at the same time, which provides a scientific basis for the quality control and evaluation of the preparation.
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