Objective: To establish an HPLC fingerprint method of Glycyrrhizae Radix et Rhizoma, and tostudy the relationship between spectrum and antioxidant activity. Methods: Fingerprint by HPLC was established on Glycyrrhizae Radix et Rhizoma from different sources. On the basis that the similaritieswith the reference fingerprintabove 0.915,25 common peaks were evaluated with hierarchical cluster analysis(HCA) and principal component analysis(PCA). The antioxidant activity was determined by 1,1-diphenyl- 2- picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl(DPPH), ferric ion reduction ability plasma(FRAP) analysis, andsalicylic acid assays.Partial least squares regression(PLSR) analysis and grey relational analysis(GRA) were used to study the relationship between HPLC fingerprint and free radical- scavenging activity. Results: The HPLC fingerprint of 25 batches of Glycyrrhizae Radix et Rhizoma was established, and 26 common peaks were determined,with the similarities above 0.915. Nine common peaks were identified by comparing to reference substances.The result of HCA showed that Glycyrrhizae Radix et Rhizoma from different sources were clustered into four categories, which was in consistent with the result of PCA.All of the samples had different degrees of antioxidant activity. The spectrum-activity relationship showed that the peaks 2,7,10,13,18,21 and 23 were positively correlated with the antioxidant activity. Conclusion: The antioxidant activity of Glycyrrhizae Radix et Rhizomais the result of the combined effects of multiple components, and peaks of 7 components are closely related to the antioxidant activity. The present study provides more comprehensive reference for quality control of Glycyrrhizae Radix et Rhizoma.
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