Objective： To develop a method of high performance liquid chromatography-variable wavelength detector-evaporative light scattering detection（ HPLC-VWD-ELSD） for simultaneous determination of gallic acid,puerarin,paeoniflorin,notoginsenoside R1,astragaloside and tanshinone ⅡA in Huangjia Ruangan granules. Methods： HPLC assay was performed on a Waters Symmetry-C18 column（ 250 mm×4.6 mm,5 μm） with a mixture of methanol and 0.1% aqueous acetic acid as the mobile phase in gradient elution at the flow rate of 1.0 mL·min-1. Detection wavelength for gallic acid,puerarin and tanshinone ⅡA was set at 270 nm and ELSD was used for detection of paeoniflorin,notoginsenoside R1 and astragaloside. The temperature of the drift tube was maintained at 70 ℃ and the nebulizing gas flow rate was 2.0 L·min-1. Results： The linear ranges of gallic acid, puerarin,paeoniflorin,notoginsenoside R1,astragaloside and tanshinone ⅡA were 1.444-28.88,2.118-42.36, 1.074-21.48,0.218 0-4.360,0.115 1-2.302 and 0.066 70-1.334 μg,respectively. The average recoveries （n=6） were 93.8% with RSD of 1.4%,98.8% with RSD of 0.55%,97.5% with RSD of 3.3%,103.0% with RSD of 4.0%,100.6% with RSD of 3.9%,96.6% with RSD of 2.3%,respectively. The contents of the above-mentioned components in 5 samples were 2.612-3.203,9.155-10.342,7.044-9.863,0.622-0.841,1.529-1.697 and 0.719-0.957 mg·g-1,respectively. Conclusion： The method is simple,rapid and reproducible. It can be used for the determination of the six components in Huangjia Ruangan granules,providing a scientific basis for the comprehensive quality control of Huangjia Ruangan granules.
WANG Zong-quan, HUANG Chao-qing, HUANG Zhi-hui, LI Yan-ying, GAO Jin, HUANG Neng-ting, LIU Jun, ZHANG Bao-xian, TENG Li-rong
. Simultaneous determination of six components in Huangjia Ruangan granules by HPLC-VWD-ELSD*[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(7)
: 1148
-1154
.
DOI: 10.16155/j.0254-1793.2021.07.05
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