Objective： To establish an HPLC method for fingerprint analysis of Yixintai granules for its quality control. Methods： The chromatographic behaviors were obtained by a Phenomenext Luna C18 column（ 250 mm×4.6 mm,5 μm） with gradient elution using 0.1% phosphoric acid solution-methanol as the mobile phase. The detection wavelength was 286 nm,the volume flow rate was 1.0 mL·min-1,and the column temperature was 30 ℃ . The samples of 10 batches of Yixintai granules were determined,the chromatographic fingerprint similarity evaluation system of Chinese medicine（ 2012 edition） was used to establish the common pattern of Yixintai granules fingerprints,and the similarity was calculated. Then the common peaks were identified by the reference chromatogram. Results： HPLC fingerprints of Yixintai granules were established by the determination of 10 batches of samples. The similarity was above 0.98. A total of 31 common peaks were calibrated. Four mutual peaks（ No. 15,25,30,31 peaks） were from Astragali Radix,seven mutual peaks（ No. 3,20,21,22,23,24, 28 peaks） were from Salviae Miltiorrhizae Radix et Rhizom,nine mutual peaks（No. 6,7,11,12,13,14,26, 27,29 peaks） were from Carthami Flos,six mutual peaks（No. 8,10,15-18 peakes） were from Descurainiae Semen. No. 5 peak was form Alismatis Rhizoma. No. 1,9 peaks were shared by Carthami Flos,and Alismatis Rhizoma,No. 2 peaks was shared by Carthami Flos,and Poria,No. 4 peaks was shared by Salviae Miltiorrhizae Radix et Rhizom. and Polyporus. The common peaks were all assigned to each medicinal material and identified by reference chromatograms：No. 12,19,21,25,30 peaks were hydroxysafflor yellow A,calycosin 7-O-glucoside, salvianolic acid B,formononetin,calycosin. Conclusion： The similarity results of 10 batches of samples indicate that the particle preparation process is stable and feasible,and the established HPLC fingerprint method is stable and reliable.
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