Objective： To establish a method for quantitative analysis of active components in plasma of Longshengzhi capsules and to study the pharmacokinetic of each component. Methods： Using HPLC-MS/MS analysis method,Agilent Rapid Resolution HD C18 column（ 2.1 mm×50 mm,1.8 μm） was adopted and the mobile phase was acetonitrile-water（ containing 0.1% formic acid） with gradient elution at a flow rate of 0.5 mL·min-1. The electrospray ion source was adopted,and the positive or negative ion mode for multiple reaction detection scan（ MRM） was selected. In detection mode,the analysis time was 6 min in positive ion mode and 3 min in negative ion mode. Results： Methodologically verified,the linear relationship of each component was good（r>0.997 7）, the extraction recoveries were in the range of 85.1%-114.5%. The matrix effect was in the range of 85.08%-112.95%, the RSD values for intra-day precision,inter-day precision and stability were below 10%,9.6% and 13%,respectively. They all met the requirements of biological sample analysis and the requirements of analysis and determination of various components in rat plasma samples. The pharmacokinetic parameters of the 7 active ingredients of Longshengzhi capsules in rats were calculated by DAS 3.0 software. The results showed that the peak time（ Tmax） of amygdalin,ferulic acid, isofraxidin,calycosin,senkyunolide A,oxypaeoniflora,and paeoniflorin was（ 0.36±0.31）h and（ 0.11±0.05）h, （0.10±0.04）h,（3.36±3.69）h,（1.07±0.80）h,（2.35±3.12）h and（ 0.60±0.44）h,respectively. The elimination half-life（ t1/2） was（ 1.02±0.46）h,（7.95±10.74）h,（32.55±15.57）h,（3.67±2.07）h,（1.19±0.12）h, （2.39±1.92）h and（ 1.50±0.12）h,respectively. Conclusion： This study provides scientific data for the study of pharmacological activity and clinical use of the main components of Longshengzhi capsules.
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