Objective： To establish a Raman spectroscopic method for rapid determining the content of calcium sulfate dihydrate in gypsum. Methods： Sodium nitrate as internal standard and sodium chloride as filler were first mixed with gypsum,which were grinded,compressed into tablets and analyzed by Raman spectrometer. Analytical conditions were as follows：the excitation wavelength was 780 nm,the laser power was 24 mW,the aperture was 50 μm slit,the lens was 10×objective,the exposure time was 2 s,the accumulation was 2 times,and the scanning range was 300~1 700 cm-1. Results： The peak intensity ratio（ I1 007/I1 068） was used for the quantitative evaluation of the gypsum level and showed a good linear relationship with mass fraction of calcium sulfate dihydrate in the range of 5%-35%. The linear regression equation was Y=0.019X-0.035 5,R2=0.999 4. The average recoveries at low, medium and high concentrations were 99.4%,97.9% and 99.1%,with RSD of 1.5%,1.1% and 1.3%,respectively. There was no significant difference between the analytical results（97.8%-102.0%） and those（98.8%-101.3%） with EDTA-2Na titration method by t test（ P=0.415,>0.05）. Conclusion： The method is rapid and accurate. It can be used as a new analytical method for quality control of medicinal gypsum.
PENG Yan, ZHANG Li-ying, JIN Yang, YANG Zhao, LU Tu-lin, JIN Chuan-shan, DU Shu-hu
. Rapid determination of calcium sulfate dihydrate in mineral gypsum by Raman spectroscopy*[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(7)
: 1266
-1271
.
DOI: 10.16155/j.0254-1793.2021.07.18
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