Objective： To prepare hydroxyethyl amylose（HEA） with different molar masses and determine their relative molecular mass and molar substitution ratio. Methods： Amylose was purified from corn starch by a butanol coagulation method. Blue value was measured by visible spectrophotometer to identify the purity of amylose. Viscosity average relative molecular mass and weight average relative molecular mass of amylose was determined by Ubbelohde viscometer and gel permeation chromatography（GPC） respectively. Amylose was etherified by adding different concentrations of ethylene oxide under basic conditions. HEA was purified by dialysis and their molar substitution ratio was measured by gas chromatography. The weight average relative molecular mass of HEA was determined by GPC. Results： Blue value of the purified amylose was 0.92,viscosity average relative molecular mass was 2.64×106 and weight average relative molecular mass was 3.021×106. When the feed molar ratio of ethylene oxide to amylose glucose units was 4∶1,8∶1,16∶1 and 32∶1,the molar substitutions of HEA were 0.18,0.50, 1.00 and 1.37,respectively. The weight average relative molecular mass of HEA was 3.318×106,3.338×106, 3.432×106 and 3.457×106,respectively. Conclusion： Amylose with high purity and good polydispersity can be purified from corn starch by butanol coagulation method,and HEA with different molar substitution degrees can be obtained by etherifying amylose using ethylene oxide of different concentrations.
XIONG Xiao-qin, ZHANG Hong-quan, FANG Chan-yu, ZHONG Jing
. Preparation and molar substitution ratio determination of hydroxyethyl amylose*[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(7)
: 1277
-1284
.
DOI: 10.16155/j.0254-1793.2021.07.20
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