Ingredient Analysis

SPE-HPLC determination of the processing process of Aconiti Radix dynamic changes and toxicity analysis of 6 aconitine alkaloids*

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  • 1. Hubei University of Science and Technology, Xianning 437000, China;
    2. The First People's Hospital of Tongshan County, Xianning 437000, China

Received date: 2020-10-23

  Online published: 2024-07-12

Abstract

Objective:To further optimize the analysis method of alkaloids in Aconiti Radix preparata, solid phase microextraction combined with high performance liquid chromatography (SPE-HPLC) was used to determine the 6 aconitine alkaloids in Aconiti Radix preparata products. Pathological section of lung and kidney after oral administration of processed Aconiti Radix extract was analyzed, so as to determine the optimum processing method and time. Methods:The processed Aconiti Radix was purified by solid-phase extraction using a strong cationic adsorption resin MCX column. The mobile phase was 0.01 mol·L-1 ammonium formate aqueous solution (formic acid 0.1%) -acetonitrile (32% tetrahydrofuran) with gradient elution, the flow rate was 1.0 mL·min-1 and the detection wavelength was 235 nm. HE staining was used to observe the morphological changes of lung and kidney tissues after oral administration of Aconiti Radix processed product extract in rats. Results:The six aconitine alkaloids could be effectively separated under the optimized conditions. The benzoylmesaconine, benzoylaconine, benzoylhypaconine, mesaconitine, hypaconitine and aconitine had a good linear relationship within their respective linear range. The recovery rate of spiked samples was between 98.1%-104.0%, and RSD<5.0%. Aconiti Radix was processed by steaming and the amount of monoester alkaloids reached the maximum at 7 h. HE staining was used to observe the morphological changes of lung and kidney tissues after oral administration of extracts from Aconiti Radix in rats. The alveolar interstitial edema led to diffuse change, a large number of inflammatory cell infiltration and thickening of the alveolar wall. The glomerulus increased in size, and the nuclei in the glomerulus was relatively reduced. The space between the glomerulus and renal vesicles became smaller, and there was no space between the lumens, which became larger. Conclusion:The dynamic changes of aconitine alkaloids in Aconiti Radix preparata products were determined by MCX with strong cation exchange resin and high performance liquid chromatography in this experiment. The best processing method of Aconiti Radix is steaming for 7 h. The method is simple, short in time and accurate in determination, which could provide scientific basis for controlling the content of toxic components and active components in Aconiti Radix preparata products.

Cite this article

BI Jian-li, CHEN Ting, JIN Wen-fang, XU Sheng, QU Zeng-yi, RAO Meng-fan, CAI Xing, WANG Qi, FAN Bao-lei . SPE-HPLC determination of the processing process of Aconiti Radix dynamic changes and toxicity analysis of 6 aconitine alkaloids*[J]. Chinese Journal of Pharmaceutical Analysis, 2021 , 41(8) : 1389 -1398 . DOI: 10.16155/j.0254-1793.2021.08.13

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