Objective： To establish the HPLC characteristic chromatogram and simultaneous determination method of multi-components of Reyanning granules,so as to provide the basis for effective quality evaluation. Methods： The analysis was performed on Welch Ultimate Phenyl-Ether（250 mm×4.6 mm,5 μm）column with mobile phase consisting of acetonitrile and 0.15% phosphoric acid at a flow rate of 1.0 mL·min-1 with gradient elution. The column temperature was 30 ℃. The detection wavelength was set at 290 nm,and the injection volume was 10 μL. The similarity of Reyanning granules was evaluated by the Traditionai Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System（2012 edition）. Then,the further quality evaluation of the drug was carried out by cluster analysis（CA）,principal component analysis（PCA）and orthogonal partial least squares discriminant analysis（OPLS-DA）. Results： The HPLC characteristic chromatogram of Reyanning granules was established and 15 common peaks were found,6 of them were indentified as monocaffeyltartaric acid,polydatin, scutellarin,chicoric acid,resveratrol and emodin,respectively. The similarities of 16 batches of samples were 0.957-0.992. The samples were classified into three categories by CA and PCA,and six components were main markers that caused differences in the different batches of samples by OPLS-DA. The resolution of 4 components of quantitative qnalysis（polydatin,scutellarin,resveratrol and emodin）was more than 1.5,the RSDs of precision, stability（30 h）and reproducibility tests were lower than 2.2%,and the average recovery rates of four compounds were between 96.4% and 98.2%. The contents of polydatin,scutellarin,resveratrol and emodin in 16 batches of Reyanning granules were 2.516-6.190,0.454 6-1.950,0.225 8-0.629 7 and 0.183 0-0.713 5 mg·g-1, respectively. Conclusion： The method of HPLC characteristic chromatogram analysis and multi-index components determination was accurate and reproducible,which could be used for the quality control and evaluation of Reyanning granules.
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