Objective： To establish determination methods for 5 dicaffeoylquinic acids and scutellarin in Dengzhan Xixin injection by multi-components with single marker method（QAMS）and external standard method（ESM）. Methods： A high-performance liquid chromatography（HPLC）method was developed on a Waters Sunfire C18（250 mm×4.6 mm,5 μm）column with[A（methanol：acetonitrile 30∶70）-B（0.1% trifluoroacetic acid solution）]（18∶82）as mobile phase. The detection wavelength for dicaffeoylquinic acids was set at 327 nm. 3, 5-O-Dicaffeoylquinic acid（1）was chosen as the internal reference substance,the relative correction factors of 4 components（2-5）were determined by multi-point correction method,including 3,4-O-dicaffeoylquinic acid（2）,erigeron B（3）,4,5-O-dicaffeoylquinic acid（4）and erigeroster（5）and the relative retention time of the chromatographic peaks of the components to 3,5-O-dicaffeoylquinic acid were calculated,respectively. The content of compounds 1-5 in 30 batches of Dengzhan Xixin injection samples were determined by relative correction factors,and the relative deviation（RAD）was used as a parameter to compare the results with that of external standard method（ESM）to verify the accuracy of QAMS. In the same system,the detection wavelength of scutellarin was set at 335 nm,and it is formulated with 3,5-O-dicaffeoylquinic acid to make a mixed reference substance,and the content of scutellarin is calculated by external standard method and the relative deviation（RAD） was used as the parameter to compare the method of Chinese Pharmacopoeia（ESM）with that of established method. Durability tests were performed with different chromatographic columns,instruments,levels of different mobile phase proportions,flow rates,temperatures and wavelengths. The relative correction factor,the relative retention time and the results were calculated respectively. The practicability of the established method was verified by comparing the relative standard deviation of the results. Results： The relative correction factors（RCF）of 4 components（2-5）were 1.140 7,1.169 1,1.017 2 and 1.351 5,respectively,and the relative retention values were 0.92,1.54,1.72 and 2.11,respectively. The relative deviation（RAD）of the contents of 5 dicaffeoylquinic acid by QAMs and determined by ESM in 30 batches of Dengzhan Xixin injection were less than 5%. The relative deviation （RAD）of the contents of scutellarin by the established method and by the Chinese Pharmacopoeia method in 30 batches of Dengzhan Xixin injection were less than 5%. Conclusion： The established content determination method can be applied to the determination of 5 dicaffeoylquinic acid and scutellarin in Dengzhan Xixin injection.
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