Metabolism Analysis·Activity Analysis·Bioassay

Experimental study on the effect of Haematococcus pluvialis on the antioxidant capacity of skeletal muscle and mitochondria*

Expand
  • 1. Physical Education Department, Xi′an Shiyou University, Xi′an 710065, China;
    2. Institute of Sports Biology, Shaanxi Normal University, Xi′an 710062, China

Received date: 2020-03-25

  Online published: 2024-07-12

Abstract

Objective: To explore the effects of Haematococcus pluvialis(HP)on the antioxidant capacity of skeletal muscle and mitochondria and the mechanism of antifatigue effect by establishing two training experimental models of mice swimming and rats treadmill. Methods: 32 male mice and rats,8 in each group,were selected,and the mice were randomly divided into swimming training group(group a),exhausted swimming group(group b),swimming ASTA group(group c)and swimming HP group(group d),and the rats were randomly divided into treadmill training group(group A),exhausted treadmill group(group B),treadmill ASTA group(group C) and treadmill HP group(group D). Mice and rats were trained for exercise according to different training scheme. At the end of the training program,skeletal muscle samples were prepared,and SOD,T-AOC,GSH-Px,CAT,-SH,MDA,Na+/K+-ATPase,Ca2+/Mg2+ATPase and other indicators were determined. Results: Compared with groups a and A(training control group),experimental animals in groups b and B showed a significant decrease (P<0.05)in skeletal muscle tissue and muscle mitochondrial antioxidant enzymes,ATPase activity and antioxidant-SH content,and a significant increase(P<0.05)in MDA content after forceful exercise. Compared with groups b and B(force exhaustion control group),animals in the astaxanthin and Haematococcus pluvialis supplementation groups(groups c,d,C and D)showed significantly higher level of skeletal muscle and mitochondrial antioxidant enzymes,ATPase activity and antioxidant-SH content(P<0.05),significantly lower MDA content(P<0.05), and significantly longer exhaustive time after force exhaustion(P<0.05 ). There were no significant differences in skeletal muscle and mitochondrial antioxidant indexes in animals in the group supplemented with Haematococcus pluvialis(groups d and D)compared with groups c and C(positive control group),but the exhaustive time was significantly longer than in animals in the astaxanthin supplemented group(P<0.05). Conclusion: Haematococcus pluvialis supplement could improve the antioxidant capacity of skeletal muscle and mitochondriathe,prolong the exhaustive time of endurance-trained mice and rats. The mechanism is closely related to its rich antioxidant astaxanthin. Haematococcus pluvialis also contains other medicinal components to delay exercise fatigue,which need further study.

Cite this article

XU Xiao-xian, XIONG Zheng-ying . Experimental study on the effect of Haematococcus pluvialis on the antioxidant capacity of skeletal muscle and mitochondria*[J]. Chinese Journal of Pharmaceutical Analysis, 2021 , 41(5) : 817 -825 . DOI: 10.16155/j.0254-1793.2021.05.08

References

[1] RUIZ MC, ESPINOSA C, PAREDES A, et al.Determining the potential of Haematococcus pluvialis Oleoresin as a rich source of antioxidants[J].Molecules, 2019, 24(22):E4073
[2] 李学艳, 王君文, 易金玉, 等 . 含虾青素的雨生红球藻粉自微乳的制备及体外评价[J/OL].上海海洋大学学报, [2020-11-24]. https://nxgp.cnki.net/kcms/detail?v=3uoqIhG8C46NmWw7YpEsKHT PvOGrUOOqX1coEOzL8AEGsJUMHAntDUKb2qI2BH7LWmKx_dB02KDZep4eXUdZ54EqXdBXa5xh&uniplatform=NZKPT
LI XY, WANG JW, YI JY, et al.Preparation and in vitro evaluation of self microemulsion of Haematococcus pluvialis powder containing astaxanthin[J/OL].J Shanghai Ocean Univ, [2020-11-24].https://nxgp.cnki.net/kcms/detail?v=3uoqIhG8C46NmWw7YpEsKHTPvOGrUOOqX1coEOzL8AEGsJUMHAntDUKb2qI2BH7LWmKx_dB02KDZep4eXUdZ54EqXdBXa5xh&uniplatform=NZKPT
[3] LORENZ RT, C YSEWSKI GR .C ommercial potential for Haematococcus microalgae as a natural source of astaxanthin[J].Trends Biotechnol, 2000, 18(4):160
[4] HORMOZI M, GHOREISHI S, BAHARVAND P.Astaxanthin induces apoptosis and increases activity of antioxidant enzymes in LS-180 cells[J].Artif Cells, 2019, 47(1):891
[5] ZHAO XY, ZHANG XW, LIU HK, et al.Enzyme-assisted extraction of astaxanthin from Haematococcus pluvialis and its stability and antioxidant activity[J].Food Sci Biotechnol, 2019, 28(6):1637
[6] RANGA RA, SARADA R, SHYLAJA MD, et al.Evaluation of hepatoprotective and antioxidant activity of astaxanthin and astaxanthin esters from microalga-Haematococcus pluvialis[J].J Food Sci Technol, 2015, 52(10):6703
[7] 王潮岗, 韩燊, 陈甄倩, 等 . 雨生红球藻抗氧化系统对活性氧的清除机制[J].水生生物学报, 2012, 36(4):804
WANG CG, HAN S, CHEN ZQ, et al.The Scavenging of reactive oxygen species with antioxidant systems in Haematococcus pluvialis[J].Acta Hydrobiol Sin, 2012, 36(4):804
[8] 刘颖芬, 辛乃宏, 李炳乾, 等.雨生红球藻虾青素对小鼠免疫调节的研究[J].食品研究与开发, 2017, 38(20):183
LIU YF, XIN NH, LI BQ, et al.Study of astaxanthin from Haematococcus pluvialis on immune regulation function in mice[J].Food Res Devel, 2017, 38(20):183
[9] 贺青华, 李勇超, 王文娟, 等.雨生红球藻虾青素对糖尿病大鼠脑损伤的干预作用[J].中国食品学报, 2019, 19(5):33
HE QH, LI YC, WANG WJ, et al.Intervention function of astaxanthin from Haematococcus pluvialis on brain injury diabetic rats[J].J Chin Inst Food Sci Technol, 2019, 19(5):33
[10] 李勇超, 赵健, 任超, 等.雨生红球藻破壁孢子粉对Ⅱ型糖尿病大鼠血糖及血脂的干预作用[J].中国食品学报 2018, 18(10): 31
LI YC, ZHAO J, REN C, et al.Intervention effect of Haematococcus pluvialis wall breaking spore powder on blood glucose and blood lipid in type 2 diabetic rats[J].J Chin Inst Food Sci Technol, 2018, 18(10):31
[11] 黄浦俊, 朱巧巧, 颜美秋, 等.破壁、未破壁雨生红球藻粉体外抗氧化活性及体内抗衰老作用及比较研究[J].中药药理与临床, 2018, 34(2):83
HUANG PJ, ZHU QQ, YAN MQ, et al.Comparative study on antioxidant activity in vitro and anti-aging effect in vivo of broken and unbroken Haematococcus pluvialis[J].Pharmacol Clin Chin Mater Med, 2018, 34(2):83
[12] LEE SJ, BAI SK, LEE KS, et al.Astaxanthin inhibits itric oxide production and inflammatory gene expression by uppressing I( kappa)B kinase dependent NF—kappa B activation[J].Mol Cells, 2003, 16(1):97
[13] 佟强, 曹建民, 周海涛 . 雨生红球藻对过度训练大鼠骨骼肌 MAPK 信号通道蛋白表达及抗氧化能力的影响[J].中国体育科技, 2016:52(4):133
TONG Q, CAO JM, ZHOU HT.Effects of haematococcus pluvialis on mitogen-activated protein kinase signaling pathways protein expression and antioxidant capacity in overtraining rat skeletal muscle[J].China Sport Sci Technol, 2016:52(4):133
[14] 王书妍, 曹旭鹏, 孟迎迎, 等 . 雨生红球藻中虾青素含量快速测定方法[J].中国油脂, 2018, 43(12):144
WANG SY, CAO XP, MENG YY, et al.A rapid determination method for astaxanthin content in Haematococcus pluvialis[J].China Oils Fats, 2018, 43(12):144
[15] AOI W, NAITO Y, SAKUMA K, et al.Astaxanthin limits exercise-induced skeletal and cardiac muscle damage in mice[J].Antioxid Redox Signal, 2003, 5(1):139
[16] CHUAN SC, JENG SD, HENG YC.Antioxidant and anti-inflammatory properties of taiwanese yam(Dioscorea japonica Thunb. var.pseudojaponica(Hayata)Yamam.)and its reference compounds[J].Food Chem, 2013, 141( 2):1087
[17] BEDFORD TG, TIPTON CM, WILSON NC, et al.Maximum oxygen consumption of rats and its changes with various experimental procedures[J].J Appl Physiol, 1979, 47(6):1278
[18] 毛雁, 韩晓燕, 熊正英, 等 . 黄精提取物对耐力训练大鼠骨骼肌组织NO-NOS体系影响的实验研究[J].北京体育大学学报, 2008, 31(9):1225
MAO Y, HAN XY, XIONG ZY, et al.Experimental research on the effect of Polygonatum sibiricum Red to NO-NOS system in skeletal muscles tissues of endurance-trained rats[J].J Beijing Sport Univ, 2008, 31(9):1225
[19] HAYASHI G, CORTOPASSI G.Oxidative stress in inherited mitochondrial diseases[J].Free Radic Biol Med, 2015, 88(7): 10
[20] POWERS SK, NELSON WB, HUDSON MB.Exercise-induced oxidative stress in humans:cause and consequences[J].Free Radic Biol Med, 2011, 51(5):942
[21] 郭新明, 吴丽君, 赵静, 等. 虾青素补充对人体急性大强度运动前后代谢的影响[J/OL]. 食品科学, 2021:1[2020-12-29]https://nxgp.cnki.net/kcms/detail?v=3uoqIhG8C46NmWw7YpEsKHTPvOGrUOOqX1coEOzL8AEpsIwq5xpssl-FnmkSegmng3YfGK8uViB_saMNgFZqXz1oXc4mdQ7z&uniplatform=NZKPT&uid=WEEvREcwSlJHSldSdmVqMDh6a1dpNjBzR1dGdXpiQU14LzJ1Yk9aSTRNST0=\$9A4hF_YAuvQ5obgVAqNKPCYcEjKensW4IQMovwHtwkF4VYPoHb KxJw!!.
GUO XM, WU LJ, ZHAO J, et al .Effects of astaxanthin supplementation on metabolism before and after acute high intensity exercise[J/OL].Food Sci, 2021:1[2020-12-29]https://nxgp.cnki.net/kcms/detail?v=3uoqIhG8C46NmWw7YpEsKHTPvOGrUOOqX1coEOzL8AEpsIwq5xpssl-FnmkSegmng3YfGK8uViB_saMNgFZqXz1oXc4mdQ7z&uniplatform=NZKPT&uid=WEEvREcwSlJHSldSdmVqMDh6a1dpNjBzR1dGdXpiQU14LzJ1Yk9aSTRNST0=\$9A4hF_YAuvQ5obgVAqNKPCYcEjKensW4IQMovwHtwkF4VYPoHb KxJw!!
[22] POWERS SK, RADAK Z, JI LL.Exercise-induced oxidative stress: past, present and future[J].J Physiol, 2016, 594(18):5081
[23] 吴开柱, 张辉 . 肢体缺血再灌注后关节软骨的组织学观察[J].中国矫形外科杂志, 2005, 13(7):524
WU KZ, ZHANG H.Histological observation of articular cartilage after limb ischemia-reperfusion[J].Chin J Orthopedics, 2005, 13(7):524
[24] GOMEZ-CABRERA MC, BORRÁS C, PALLARDÓ FV, et al.Decreasing xanthine oxidase-mediated oxidative stress prevents useful cellular adaptations to exercise in rats[J].J Physiol, 2005, 567(Pt 1):113
[25] PEAKE J, SUZ UKI K .Ne utrophil activation, a ntioxidant supplements and exercise-induced oxidative stress[J].Exerc Immunol Rev, 2004, 10(1):129
[26] 朱晓东 .葛根黄酮对大强度运动大鼠骨骼肌氧化应激损伤的保护作用及其机制研究[J].美食研究, 2020, 37(3):78
ZHU XD.Protective effect of pueraria flavone on oxidative stress injury of skeletal muscle in rats after high intensity exercise and its mechanism[J].Food Res, 2020, 37(3):78
[27] 邓树勋, 王健, 乔德才 . 运动生理学[M].北京:高等教育出版社, 2015:233
DENG SX, WANG J, QIAO DC.Sports Physiology[M].Beijing: Higher Education Press, 2015:233
[28] 张蕴琨, 丁树哲.运动生物化学[M].北京:高等教育出版社, 2014:125
ZHANG YK, DING SZ.Sports Biochemistry[M].Beijing:Higher Education Press, 2014:125
[29] 王耀东, 邱丽, 孙梦佳 . 壳聚糖通过减少自由基堆积提高对运动训练小鼠抗疲劳能力的实验研究[J].天津体育学院学报, 2020, 35(4):434
WANG YD, QIU L, SUN MJ.Experimental study on chitosan improving anti fatigue ability of exercise training mice by reducing accumulation of free radicals[J].J Tianjin Inst Phys Educ, 2020, 35(4):434
[30] 耿雪, 李志慧, 谭锐, 等.运动与氧化应激[J].军事医学, 2018, 42(8):631
GENG X, LI ZH, TAN R, et al.Exercise and oxidative stress[J].Milit Med, 2018, 42(8):631
[31] 杨晓磊, 刘亮, 龚玉根, 等 . 运动疲劳与氧化应激[J].中国中医药现代远程教育, 2020, 18(18):88
YANG XL, LIU L, GONG YG, et al.Exercise fatigue and oxidative stress[J].Chin Med Mod Dis Educ China, 2020, 18(18):88
[32] 窦勇, 吴琳, 闫永芳, 等. 雨生红球藻积累虾青素的分子基础与人工诱导调控研究进展[J/OL]. 水产科学, 2021 :1[2020-12-23] https://nxgp.cnki.net/kcms/detail?v=3uoqIhG8C46NmWw7YpEsKHTPvOGrUOOqX1coEOzL8AEMIAwtSPci0fO344_VijMRg4rm3-D2-A79hXWq7znt-Pk8ZtMnqxIH&uniplatform=NZKPT&uid=WEEvREcwSlJHSldSdmVqMDh6a1dpNjBzR1dGdXpiQU14LzJ1Yk9aSTRNST0=$9A4hF_YAuvQ5obgVAqNKPCYcEjKensW4IQMovwHtwkF4 VYPoHbKxJw!!.
DOU Y, WU L, YAN YF, et al.Research progress on molecular basis.Research progress on molecular basis and artificial induction regulation of astaxanthin accumulation in Haematococcus pluvialis[J/OL].Fish Sci, 2021:1[2020-12-23]https://nxgp.cnki.net/kcms/detail?v=3uoqIhG8C46NmWw7YpEsKHTPvOGrUOOqX1coEOzL8AEMIAwtSPci0fO344_VijMRg4rm3-D2-A79hXWq7znt-Pk8ZtMnqxIH&uniplatform=NZKPT&uid=WEEvREcwSlJHSldSdmVqMDh6a1dpNjBzR1dGdXpiQU14LzJ1Yk9aSTRNST0=$9A4hF_YAuvQ5obgVAqNKPCYcEjKensW4IQMovwHtw kF4VYPoHbKxJw!!
[33] PENISLUSSHIYAN S, CHITRA L, ANCY I, et al.Novel antioxidant astaxanthin-s-allyl cysteine biconjugate diminished oxidative stress and mitochondrial dysfunction to triumph diabetes in rat model[J].Life Sci, 2020, 245(1):117367
[34] 江利华, 柳慧芳, 郝光飞, 等.虾青素抗氧化能力研究进展[J].食品工业科技, 2019, 10(5):350
JIANG LH, LIU HF, HAO GF, et al.Research progress in antioxidant capacity of astaxanthin[J].Sci Technol Food Ind, 2019, 10(5):350
[35] W U D, XU H, CH E N J Y, e t al .Ef fe cts of as t axant hi n supplementation on oxidative stress a systematic review and meta-analysis of randomized controlled trials[J].Int J Vitam Nutr Res, 2020, 90(1-2):179
[36] 吴丽君, 郭新明 . 虾青素及运动对人体抗氧化能力、血乳酸、血尿酸代谢的影响[J].体育科学, 2017, 37(1):62
WU LJ, GUO XM.Effects of astaxanthin and exercise on human antioxidant capacity, blood lactic acid and blood uric acid metabolism[J].Sports Sci, 2017, 37(1):62
Outlines

/