Objective： To establish an inductively coupled plasma mass spectrometry（ICP-MS）method for determination of seven harmful elements（63Cu,52Cr,77Se,208Pb,75As,114Cd and 202Hg）in Rhei Radix et Rhizoma. By comparing the determination results,and preliminarily evaluating the risk of adding rhubarb medicinal materials, the usage and addition criteria of rhizome of Rheum officinale Baill. and Rheum tanguticum Maxim. ex Balf. in compound preparations were provided. Methods： The samples were diluted and capacitated with ultra-pure water after ultra-high-pressure microwave digestion. Mercury specimens were diluted and volume fixed directly with ultrapure water without taking acid treatment after digestion. The blank solution was prepared in the same way. By selecting suitable isotopes,75Ge,115In and 209Bi as internal standard solutions to eliminate matrix interference, ICP-MS method with KED mode（collision reaction cell technology for kinetic energy discrimination）was used to determine the contents of seven hazardous elements（63Cu,52Cr,77Se,208Pb,75As,114Cd and 202Hg）. Results： The linearity of 7 elements was good（r≥0.997）. The RSDs of the injection precision test of the 7 elements at high, medium and low concentrations were in the range of 2.7%-5.4%,the average recoveries were in the range of 90.3%-100.4% with RSDs of 1.8%-5.3%,and the digested solution was stable in 24 h. 13 batches of Rheum officinale Baill. in 1 batch of samples exceeded 4.4% of the standard for cadmium,1 batch of samples exceeded 3.5% of the standard for mercury,while the other 5 elements（63Cu,52Cr,77Se,208Pb,75As）conformed to the standards. The results of risk assessment showed that the overall pollution level of heavy metals and harmful element in 13 batches of Rheum officinale Baill. was low,the average weekly intake of seven heavy metals and harmful elements in rhubarb was less than theoretical value specified by the World Health Organization（WHO）. The overall risk was at an acceptable level,but there were some differences in exposure levels among people with different body types and intakes,which should be taken into account. Conclusion： This method is easy operated and rapid with completely sample digestion and low risk of contamination. The quantitative analysis of seven trace elements in rhubarb herbs can be effectively performed by determining the prepared standard solution and further optimizing the concentration of internal standard elements to ensure high sensitivity and low interference. Based on the results of the risk assessment,the risks arising from the residues of harmful elements in medicinal rhubarb herbs were scientifically evaluated.
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