Objective： To study the separation characteristics of drugs in capillary and to develop a novel preconcentration method based on the combination of pH-mediated stacking and sweeping micellar electrokinetic chromatographic techniques（PMS-sweeping MEKC）for simply but efficiently concentrating cardiovascular medicines,including nitrendipine,nimodipine and nifedipine in a high-salt blood sample matrix by capillary electrophoresis. Methods： The effects of experimental parameters on the separation and detection system was investigated. The optimum conditions included the MEKC buffer with the composition of 23 mmol·L-1 SDS-3.3%（v/v）methanol-30 mmol·L-1 borax-1 mmol·L-1 potassium dihydrogen phosphate running buffer（pH 9.10）. The sample injection time was 60 s in a high voltage of 15 kPa,and then the phosphate acid injection time was 80 s in a high voltage of 15 kPa. Results： Baseline separation and high sensitivity detection of three cardiovascular drugs,nitrendipine,nimodipine and nifedipine,could be achieved within 9 minutes. With the dual preconcentration method,a magnitude detection sensitivity of 180-210 fold increase could be observed compared with the conventional capillary electrophoresis. The detection limit of 0.12~0.14 mg·L-1 and recovery efficlency of 95.8%-103.5% for these three medicines in cardiovascular medicine products and the human blood were achieved. Conclusion： The method is reproducible,sensitive and high separation,with high peak capacity,and is suitable for the simultaneous separation and detection of multiple drug components.
YU Sha, WANG Jing-jing, LIU Yan-fei
. Dual concentration capillary electrophoresis by pH-mediated stacking-sweeping micellar electrokinetic chromatography in determination of cardiovascular medicines[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(5)
: 899
-905
.
DOI: 10.16155/j.0254-1793.2021.05.19
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