Standard Deliberation

Qualitative analysis of chromatographic peaks based on liner calibration with two reference substances-taking Yinzhihuang capsules as an example

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  • Sichuan Institute for Food and Drug Control, Chengdu 611731, China

Received date: 2020-06-28

  Online published: 2024-07-12

Abstract

Objective: To analyze chlorogenic acid,neo-chlorogenic acid,crypto-chlorogenic acid,geniposide and p-hydroxyacetophenone in Yinzhihuang capsules by HPLC,to predict the retention time using dualstandard linear correction method,and to investigate the feasibility of the method in qualitative analysis of chromatographic peaks. Methods: The actual retention time of 5 components in Yinzhihuang capsules on 16 different chromatographic columns were determined. After screening,the dual-label compounds were selected as neo-chlorogenic acid and p-hydroxyacetophenone,and the dual-standard linear correction method was used to predict the retention time and the method was verified on the other 4 chromatographic columns. At the same time, chlorogenic acid was selected as the reference substance,and the relative retention time method was used to predict and analyze the retention time. Results: After comparison,the dual-standard linear calibration method had higher retention time prediction accuracy(96.36%) and higher column adaptability(11 columns). Conclusion: The dual-standard linear correction method can be used for qualitative analysis and can be used as a new alternative reference method.

Cite this article

YANG Chao, LI Yang, ZHOU Juan . Qualitative analysis of chromatographic peaks based on liner calibration with two reference substances-taking Yinzhihuang capsules as an example[J]. Chinese Journal of Pharmaceutical Analysis, 2021 , 41(5) : 906 -912 . DOI: 10.16155/j.0254-1793.2021.05.20

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