Objective： To analyze chlorogenic acid,neo-chlorogenic acid,crypto-chlorogenic acid,geniposide and p-hydroxyacetophenone in Yinzhihuang capsules by HPLC,to predict the retention time using dualstandard linear correction method,and to investigate the feasibility of the method in qualitative analysis of chromatographic peaks. Methods： The actual retention time of 5 components in Yinzhihuang capsules on 16 different chromatographic columns were determined. After screening,the dual-label compounds were selected as neo-chlorogenic acid and p-hydroxyacetophenone,and the dual-standard linear correction method was used to predict the retention time and the method was verified on the other 4 chromatographic columns. At the same time, chlorogenic acid was selected as the reference substance,and the relative retention time method was used to predict and analyze the retention time. Results： After comparison,the dual-standard linear calibration method had higher retention time prediction accuracy（96.36%） and higher column adaptability（11 columns）. Conclusion： The dual-standard linear correction method can be used for qualitative analysis and can be used as a new alternative reference method.
YANG Chao, LI Yang, ZHOU Juan
. Qualitative analysis of chromatographic peaks based on liner calibration with two reference substances-taking Yinzhihuang capsules as an example[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(5)
: 906
-912
.
DOI: 10.16155/j.0254-1793.2021.05.20
[1] 孙磊, 金红宇, 逄瑜, 等.双标多测法Ⅰ—双标线性校正技术用于色谱峰的定性[J].药物分析杂志, 2013, 33(8):1424
SUN L, JIN HY, PANG Y, et al. Two reference substances for determination of multiple components(Ⅰ): linear calibration using two reference substances for identification of chromatographic peaks[J].Chin J Pharm Anal, 2013, 33(8): 1424
[2] 陈蓉, 张超, 张华锋, 等.双标线性校正法辅助色谱峰定性用于双黄连制剂的多组分分析[J].中国实验方剂学杂志, 2018, 24(8):40
CHEN R, ZHANG C, ZHANG HF, et al.Determination of multiple components in Shuanghuanglian preparations by liner calibration with two reference substances assisted with chromatographic peaks identification[J].Chin J Exp Tradit Med Form, 2018, 24(8): 40
[3] 中华人民共和国药典2015 年版. 第一增补本[S].2018:112
ChP 2015.The First SupplementhP 2015.The First Supplement[S].2018: 112
[4] 叶其旺. 高效液相色谱法同时测定茵栀黄颗粒中4 种成分[J].新中医, 2015, 47(9):206
YE QW.Simultaneous determination of four components in Yinzhihuang granules by HPLC[J].J New Chin Med, 2015, 47(9): 206
[5] 彭明丽, 赵冠人, 温筱煦.HPLC 法同时测定茵栀黄颗粒中黄芩苷、木犀草素和绿原酸的含量[J].中国药房, 2015, 26(6):837
PENG ML, ZHAO GR, WEN XX.Simultancous determination of baicalin, lutcolin and chloroginic acid in Yinzhihuang granules by HPLC[J].J China Pharm, 2015, 26(6): 837
[6] 陈永刚, 马传学, 黄成琼, 等.高效液相色谱-波长切换法同时测定茵栀黄颗粒中的7 种有效成分[J].中国医院药学杂志, 2012, 32(11): 894
CHEN YG, MA CX, HUANG CQ, et al.Simultaneous determination of 7 active components in Yinzhihuang granules by HPLC with wavelength switching[J].Chin J Hosp Pharm, 2012, 32(11): 894
[7] 任永申, 张萍, 杜晓曦, 等.基于HPLC 指纹图谱的茵栀黄注射液质量一致性和稳定性研究[J].中草药, 2008, 29(6): 837
REN YS, ZHANG P, TU XX, et al.Study on the quality consistency and stability of Yinzhihuang injection based on HPLC fingerprint[J].Chin Tradit Herb Drugs, 2008, 29(6): 837
[8] 蒋道英, 刘献洋, 侯佳伟, 等.HPLC 测定茵栀黄注射液中木犀草苷的含量[J].中成药, 2008, 30(3): 453
JIANG DY, LIU XY, HOU JW, et al.Determination of luteolin in Yinzhihuang injection by HPLC[J].Chin Tradit Pat Med, 2008, 30(3):453
[9] 靳守东. 茵栀黄注射液的化学指纹图谱与质量控制研究[D].北京:中国人民解放军军事医学科学院, 2005
QI SD.Study on Chemical Fingerprinting and Quality Control of Yinzhihuang Injection[D].Beijing:PLA Academy of Military Medical Sciences, 2005
[10] 刘凯, 李三鸣, 何媛, 等.HPLC 法测定茵栀黄软胶囊中黄芩苷、栀子苷和绿原酸的含量[J].沈阳药科大学学报, 2005, 22(2): 122
LIU K, LI SM, HE Y, et al.Deternimation of the contents of baicalin and geniposide and chlorogenic acid in Yinzhihuang soft capsules by HPLC[J].J Shenyang Pharm Univ, 2005, 22(2): 122
[11] 刘武占, 卢英翠.HPLC 法测定茵栀黄胶囊中黄芩苷的含量[J].山东医药工业, 2001, 20(5): 21
LIU WH, LU YC.Determination of baicalinin Yinzhihuang capsule by HPLC[J].Shandong Pharm Ind, 2001, 20(5): 21
[12] 张英. 高效液相色谱法测定茵栀黄注射液成分的研究[J].北华大学学报(自然科学版), 2002, 3(3): 202
ZHANG Y.Determination of Yinzhihuang injection by HPLC[J].J Beihua Univ(Nat Sci Ed), 2002, 3(3): 202
[13] 韩梅海, 成爱华, 王江梅.RP-HPLC 法测定茵栀黄注射液中黄芩苷的含量[J].中成药, 2002, 24(4): 313
HAN MH, CHENG AH, WANG JM.Objective to establish a RPHPLC method for the determination of baicalin in Yinzhihuang injection[J].Chin Tradit Pat Med, 2002, 24(4): 313
[14] 徐强. HPLC 法同时测定茵栀黄分散片、颗粒、胶囊中绿原酸、栀子苷与黄芩苷[J].中成药, 2013, 35(5): 967
XU Q.Simultaneous determination of chlorogenic acid, geniposide and baicalin in Yinzhihuang dispersible tablets, granules and capsules by HPLC[J].Chin Tradit Pat Med, 2013, 35(5): 967
[15] 鲁晓蓉, 桂双英.HPLC 法测定茵栀黄软胶囊的含量[J].安徽医药, 2008, 12(4): 28
LU XR, GUI SY.Determination of the content of Yinzhihuang soft capsule by HPLC[J].Anhui Med, 2008, 12(4): 28
