Objective: To investigate the protective effect of eriocitrin from myocardial ischemia-reperfusion injury (MIRI) in rats,and explore its action mechanism. Methods: Sixty healthy SD rats were collected and randomly divided into sham operation group,model group,low-dose,medium-dose,high-dose eriocitrin groups (erioctrin 8,16,32 mg·kg-1) and diltiazem group,with 10 rats per group. MIRI model was constructed by surgical ligation method. The low-dose,medium-dose,high-dose eriocitrin groups and diltiazem group were given administration at 1 week before modeling,while model group and sham group were given the same volume of normal saline. After reperfusion,pathological changes of myocardial tissues were observed by hematoxylin-eosin (HE) staining. The apoptosis of cardiomyocytes was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining. The levels of serum tumor necrosis factor α (TNF-α),interleukin 6 (IL-6), interleukin 1β (IL-1β),cardiac troponin I (cTnI),myoglobin (Mb) and creatine kinase MB (CK-MB) were detected by enzyme-linked immunosorbent assay (ELISA). The expressions of c-Myc,Bcl-2 associated X protein (Bax),B-cell lymphoma-2 (Bcl-2),cysteinyl aspartate specific protease-3 (caspase-3),cleaved caspase-3, Janus kinase 2 (JAK2),phosphorylated JAK2 (p-JAK2),signal transducer and activator of transcription 3 (STAT3) and phosphorylated STAT3 (p-STAT3) proteins in myocardial tissues were detected by Western blot. Results: compared with sham group,levels of HR,LVEF,FS,LVSP,LVWT and c-Myc were significantly decreased (P<0.05), while cTnI,Mb,CK-MB,TNF-α, IL-6, IL-1β, Bax/Bcl-2, cleaved caspase-3/caspase-3, p-JAK2/JAK2 and p-STAT3/STAT3 levels were significantly increased in model group (P<0.05). Compared with model group,levels of HR,LVEF,FS,LVSP,LVWT and c-Myc were significantly increased (P<0.05), while cTnI, Mb, CK-MB,TNF-α, IL-6, IL-1β, Bax/Bcl-2, cleaved caspase-3/caspase-3, p-JAK2/JAK2 and p-STAT3/STAT3 levels were significantly decreased in 16 mg·kg-1 and 32 mg·kg-1 eriocitrin groups,and diltiazem group (P<0.05). Conclusion: Eriocitrin may play a myocardial protection role in MIRI by reducing the expressions of pro-inflammatory cytokines and simultaneously regulating JAK2/STAT3 signaling pathways to inhibit myocardial apoptosis.
LI Li-li, YANG Gui-zhi, TIAN Zhi-bin, ZHAO Xu, SUN Jiu-yan
. Protective effect of eriocitrin from myocardial ischemia-reperfusion injury in rats*[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(9)
: 1505
-1512
.
DOI: 10.16155/j.0254-1793.2021.09.03
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