Metabolism Analysis

Determination of cannabidiol and its pharmacokinetics in rat plasma by ultra performance liquid chromatography-triple quadrupole mass spectrometry

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  • School of Pharmacy,Binzhou Medical University,Yantai 264003,China

Received date: 2020-12-11

  Online published: 2024-07-15

Abstract

Objective: To establish a method for determination of cannabidiol in pharmacokinetics study of rat plasma based on protein precipitation coupled with ultra performance liquid chromatography-triple quadrupole mass spectrometry. Methods: Cannabidiol was effectively extracted by acetonitrile with a ratio of plasma-acetonitrile (1∶3),and was separated with a Waters ACQUITY HSS T3 column (100 mm×2.1 mm,1.7 μm) using acetonitrile-5 mmol·L-1 ammonium acetate (80∶20) as the mobile phase. Under the electrospray negative ion mode,the multi-reaction monitoring mode was used for qualitative and quantitative analysis of cannabidiol. Results: The results indicated that cannabidiol in plasma had a good linear relationship in a range of 2.5-1 500 ng·mL-1(r2≥ 0.998). The lower detection limit was 2.5 ng·mL-1. The intra-and inter-batch precision were less than 15%,the accuracy RE was within ±12% and the average recoveries was 96.1%-100.1% for spiked plasma. The matrix effect and plasma stability could meet methodological requirements for biological sample analysis. The established method was successfully used in pharmacokinetics of cannabidiol in rat plasma. The results showed Cmax in plasma was 70.4 ng·mL-1 and oral relative bioavailability was 22.6%. Conclusion: This method is accurate,sensitive,simple and fast. It can help the development of new drug delivery systems for cannabidiol.

Cite this article

FU Xin-zhen, XIE Ze-ping, LI Zhi, GUO Lin, LIU Ming, ZHANG Shu-min, KOU Li-juan . Determination of cannabidiol and its pharmacokinetics in rat plasma by ultra performance liquid chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Pharmaceutical Analysis, 2021 , 41(9) : 1513 -1518 . DOI: 10.16155/j.0254-1793.2021.09.04

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