Objective: To develop an UPLC method for simultaneous determination of seven triterpenoids:antcin K,antcin C,antcin H,antcin B,antcin A,dehydrosulphurenic acid(DSA),and dehydroeburicoic acid(DEA) in Antrodia cinnamomea fruiting bodies. Methods: The ACQUITY UPLC BEH C18 column(100 mm×2.1 mm,1.7 μm) was adopted,the mobile phase was acetonitrile-0.2% formic acid aqueous solution,with a gradient elution. The flow rate was 0.3 mL·min-1,the detection wavelength was set at 254 nm,and the column temperature was set at 30 ℃ . Results: Antcin K,antcin C,antcin H,antcin B,antcin A,DSA,and DEA exhibited good linear correlation in the given concentration ranges(r=0.999 5-0.999 8). The average recoveries(n=6) were 97.9%,98.2%,97.2%,97.8%,98.3%, 98.1%,and 97.5% respectively(RSD<2.0%). The RSDs of precision were less than 1.5% for the seven components, and the RSDs of reproducibility were less than 2.0%. The solution was stable within 48 h. The determination results of 18 batches of samples showed that the contents of ergostanes were high(7.95%-14.91%) in Antrodia cinnamomea fruiting bodies,while the lanostanes were relatively low(0.36%-1.08%). Conclusion: This method is proved by methodology validation that it can be used for determination of triterpenoids in Antrodia cinnamomea.
LI Qin, YE Cai-fa, YOU Yong-ji
. Simultaneous determination of seven triterpenoids in Antrodia cinnamomea fruiting bodies by UPLC[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(9)
: 1576
-1582
.
DOI: 10.16155/j.0254-1793.2021.09.10
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