Objective: To establish an high-throughput sequencing method for molecular identification of mixed powders of 11 species of Aconitum medicinal materials. Method: Using high-throughput sequencing technology, total DNA from 11 kinds of Aconitum medicinal powder samples was extracted following PCR amplification of ITS2 fragments. Paired-end sequencing of the DNA fragment was performed using IlluminaMiSeq platform. Finally,using FLASH,QIIME,GraPhlAn and MEGA 6.0 software,the sequence was sorted out and the species in the mixed powder were identified by cluster analysis. Results: A total of 82 348 high-quality ITS2 sequences were obtained in the mixed samples. The Aconitum species contained 69 814 sequences. 8 species were found including Radix Aconiti kusnezoffii,Radix Aconiti Carmichaelii ,Radix Aconiti Yinschanicum,Radix Aconiti jeholense,Radix Aconiti hemsleyanum,Radix Aconiti carmichaelii,Radix Aconiti scaposum and Radix Aconiti sinomontanum. But Radix Aconiti coreanum ,Radix Aconiti sungpanense and Radix Aconiti henryi were not identified successfully. Conclusion: Using ITS2 as a barcode,the high-throughput sequencing technology can identify 8 species in the mixed powder of Aconitum medicinal materials,providing new ideas for the identification of mixed medicinal materials.
Sa-qie-er, BAO Shu-yin, Wu-ren-ji-ru-la, AO Wu-li-ji, BAO Gui-hua, Xu-liang, ZHANG Ting-ting
. Identification of mixed powder of 11 species of Aconitum Linnaeus medicinal materials based on high-throughput sequencing technology*[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(9)
: 1598
-1604
.
DOI: 10.16155/j.0254-1793.2021.09.13
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