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Simultaneous determination of five phenolic acids in Xanthii Fructus by QAMS*

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  • 1. School of Pharmaceutical Sciences,Shandong First Medical University & Shandong Academy of Medical Sciences, Taian,271016,China;
    2. Taian Institute for Food and Drug Control,Taian,271016,China

Received date: 2021-01-18

  Online published: 2024-07-15

Abstract

Objective: To establish a method for the simultaneous determination of five phenolic acids in the traditional Chinese medicine Xanthii Fructus with the quantitative analysis of multi-components by single marker (QAMS). Methods: The analysis was performed on an HYPERSIL C18 column(4.6 mm×250 mm,5 μm), the detection wavelength was 254 nm,the column temperature was 30 ℃,the flow rate was 1.0 mL·min-1, the injection volume was 10 μL,the mobile phase was 0.1% phosphoric acid(A)-methanol(B)with gradient elution. Using chlorogenic acid as the internal reference substance,the relative correction factors(RCFs)of caffeic acid,isochlorogenic acid A,cynarin and isochlorogenic acid C to chlorogenic acid were determined. At the same time,QAMS and the conventional external standard method were adopted to determine the contents of five phenolic acids in the Xanthii Fructus,and the results of the two methods were compared. Results: There was no significant difference between the content determination results of QAMS and the external standard method,and the established relative correction factors(RCFs)had good durability. Conclusion: QAMS is feasible and scientific for the determination of phenolic acids in the traditional Chinese medicine Xanthii Fructus. It can be used for the quality control of the traditional Chinese medicine Xanthii Fructus.

Cite this article

ZHANG Zuo-hua, CHU Guang-song, WANG Hai-yan, LIU Yuan-yuan, LI Ke, QI Yong-xiu, PENG Ting-tao . Simultaneous determination of five phenolic acids in Xanthii Fructus by QAMS*[J]. Chinese Journal of Pharmaceutical Analysis, 2021 , 41(9) : 1612 -1620 . DOI: 10.16155/j.0254-1793.2021.09.15

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