Objective: To establish an HPLC multi-wavelength switching method for the simultaneous determination of echinacoside,paeonia lactiflora,verbascoside,salvianolic acid B,paeonol,and tanshinone ⅡA in Congrong Shujing granules. Methods: An Agilent Zorbax Eclipse XDB-C18column（250 mm×4.6 mm,5 μm） was used, with acetonitrile-0.1% phosphoric acid solution as the mobile phase in gradient elution. The flow rate was 1.0 mL·min-1,the column temperature was 25 ℃,the injection volume was 20 μL,and the detection wavelengths were set at 330 nm（echinacoside and verbascoside）,230 nm（paeoniflorin）,286 nm（salvianolic acid B and paeonol） and 270 nm（tanshinone ⅡA）. Results: Echinacoside,paeoniflorin,verbascoside,salvianolic acid B,paeonol and tanshinone ⅡA showed good linearity in the ranges of 18.18-181.8 μg·mL-1（r=0.999 8）,28.86-288.6 μg·mL-（1 r=0.999 8）,0.576 0-5.760 μg·mL-（1 r=0.999 5）,32.96-329.6 μg·mL-（1 r=0.999 7）,31.97-319.7 μg·mL-（1 r=0.999 0）,1.120-11.20 μg·mL-（1 r=0.999 8）,respectively . The average recovery（n=6） were 98.7%, 99.4%,98.6%,99.0%,98.8%,99.1% with RSDs of 1.0%,0.92%,1.6%,0.85%,1.0%,and 1.7%,respectively. The content ranges of the above-mentioned components in 3 samples were 3.571-3.702 mg·g-1,6.692-6.911 mg·g-1,0.071-0.089 mg·g-1,7.343-7.947 mg·g-1,2.201-2.259 mg·g-1 and 0.041-0.055 mg·g-1,respectively. Conclusion: This method can be used as the quality standard of Congrong Shujing granules to simultaneously determine the contents of six main components.
HONG Li-ting, LIU Xiu-mian, CHEN Dan, HUANG Qing-de, YU Wen-jing, XONG Chao-dong, CAI Jing
. Simultaneous determination of the six main components in Congrong Shujing granules by HPLC with multi-wavelength switching*[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(4)
: 579
-585
.
DOI: 10.16155/j.0254-1793.2021.04.03
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