Standard Deliberation

Study on the multi-component determination of Chuanxiong Rhizoma based on fingerprint and chemical pattern recognition

Expand
  • 1. College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;
    2. Jiangsu Institute for Food and Drug Control,Nanjing 210019,China;
    3. Jiangsu Engineering Research Center for Efficient Delivery System of TCM,Nanjing 210023,China

Revised date: 2020-09-21

  Online published: 2024-07-15

Abstract

Objective: To establish HPLC fingerprint and determination method of active ingredients in Chuanxiong Rhizoma,which can be used to improve the quality control standard of Chuanxiong Rhizoma. Methods: The fingerprints of 18 batches of Chuanxiong Rhizoma were constructed. Based on multivariate statistical analysis (principal component analysis and cluster analysis),the quality differences of 18 batches of medicinal materials were comprehensively evaluated,the main chromatographic peaks affecting the quality differences were explored, and the content of their active ingredients was determined. Kromasil C18(250 mm×4.6 mm,5 μm)was used as chromatographic column with acetonitrile as mobile phase A and 0.1% phosphoric acid solution as mobile phase B for gradient elution at a flow rate of 1 mL·min-1. The column temperature was 30 ℃ and the detection wavelength was 280 nm. Results: 13 common peaks were identified by fingerprint,and 7 peaks were identified by comparison. The similarities of 18 batches of herbs were above 0.97. The results of principal component scores were consistent with those of cluster analysis,and the samples were divided into three categories. The contents of ferulic acid, senkyunolide I,senkyunolide A and ligustilide in 18 batches of Chuanxiong Rhizoma were 0.17%-0.41%, 0.08%-0.21%,0.63%-3.82% and 1.02%-2.12%,respectively. Conclusion: The established analytical method is stable and reliable,which is more controllable than the quality standard of Chuanxiong Rhizoma in Chinese Pharmacopoeia(2020 Edition).

Cite this article

LIU Yu-ying, CHEN Jian, HU Hao-bin, LI Jun-song . Study on the multi-component determination of Chuanxiong Rhizoma based on fingerprint and chemical pattern recognition[J]. Chinese Journal of Pharmaceutical Analysis, 2021 , 41(4) : 685 -693 . DOI: 10.16155/j.0254-1793.2021.04.16

References

[1] 中华人民共和国药典2015 年版.一部[S].2015:40
ChP 2015.Vol ⅠhP 2015.Vol Ⅰ[S].2015:40
[2] 李晓念,陈幸,李彬,等.川产道地药材川芎中重金属的来源途径研究[J].安徽农业科学,2010,38(34):19361
LI XN,CHEN X,LI B,et al.Study on the source of heavy metal elements in Rhizoma Ligustici Chuanxiong[J].J Anhui Agr Sci,2010,38(34):19361
[3] XIA R,LI M,CHENG P,et al.Ligusticum chuanxiong Hort.:a review of chemistry and pharmacology[J].Pharm Biol,2011,49(11):1180
[4] 韩炜. 川芎的化学成分与药理作用研究进展[J].中国现代中药,2017,19(9):1341
HAN W.Advances in chemical constituents and pharmacological effects of Ligusticum chuanxiong[J].Mod Chin Med,2017,19(9):1341
[5] 张丽娟,刘继勇,姚翀,等.洋川芎内酯类化合物药理作用研究进展[J].中国药学杂志,2015,50(13):1081
ZHANG LJ,LIU JY,YAO C,et al.Research progress in pharmacological activities of senkyunolides[J].Chin Pharm J,2015,50(13):1081
[6] 刘娟,冯芮,蒲忠慧,等.指纹图谱结合HPLC 定量分析在中药川芎质量评价中的应用研究[J].中药材,2019,42(2):353
LIU J,FENG R,PU ZH,et al.Application of fingerprint combined with HPLC quantitative analysis in quality evaluation of Ligusticum chuanxiong[J].J Chin Med Mater,2019,42(2):353
[7] 冯玉,甄亚钦,田伟,等.基于UPLC 指纹图谱和化学计量学相结合的川芎质量研究[J].中国现代应用药学,2019,36(23):2903
FENG Y,ZHEN YQ,TIAN W,et al.Study on the quality of Chuanxiong Rhizoma based on UPLC fingerprint and stoichiometry[J].Chin J Mod Appl Pharm,2019,36(23):2903
[8] 孙立磊,王帅,包永睿,等.多波长覆盖融合指纹图谱评价不同产地川芎药材差异性[J].中国实验方剂学杂志,2016,22(5):75
SUN LL,WANG S,BAO YR,et al.Difference of Chuanxiong Rhizoma from different regions based on multiple wavelength coverage fusion fingerprint[J].Chin J Exp Tradit Med Form,2016,22(5):75
[9] 马天成,崔思娇,张靓琦,等.川芎的UPLC 指纹图谱研究[J].中国药房,2013,24(15):1389
MA TC,CUI SJ,ZHANG LQ,et al.Study on UPLC fingerprints of Ligusticum chuanxiong[J].China Pharm,2013,24(15):1389
[10] 付小梅,孙菡,刘婧,等.基于聚类分析和主成分分析的栀子指纹图谱研究[J].中草药,2018,49(19):4653
FU XM,SUN H,LIU J,et al.HPLC fingerprint of fruits of Gardenia Fructus based on cluster analysis and principal component analysis[J].Chin Tradit Herb Drugs,2018,49(19):4653
[11] 金玉青,吕光华,魏伯平,等.川芎药材贮藏期间化学成分的变化[J].中药材,2013,36(1):38
JIN YQ,LÜ GH,WEI BP,et al.Chemical change of Chuanxiong raw materials during storage[J].J Chin Med Mater,2013,36(1):38
[12] 王露露,孙倩怡,杨慧海,等.模式识别及其在中药质量评价中的应用[J].中草药,2016,47(23):4282
WANG LL,SUN QY,YANG HH,et al.Pattern recognition and its application in quality assessment of Chinese materia medica[J].Chin Tradit Herb Drugs,2016,47(23):4282
[13] 肖蓉,张志斐,袁志芳,等.河北道地药材黄芩指纹图谱的研究[J].药物分析杂志,2007,27(7):1018
XIAO Y,ZHANG ZF,YUAN ZF,et al.Studies on HPLC fingerprints of Scutellaria baicalensis Georgi.from Rehe of Hebei province[J].Chin J Pharm Anal,2007,27(7):2018
[14] 鄢玉芬,徐双美,梁乙川,等.HPLC 法测定不同贮藏条件下川芎中10种化学成分的含量[J].中国药房,2019,30(6):92
YAN YF,XU SM,LIANG YC,et al.Content determination of 10 kinds of chemical components in Ligusticum chuanxiong under different storage conditions by HPLC[J].China Pharm,2019,30(6):92
[15] 杨扬,潘勤,丁黎,等.QNMR 法测定川芎药材中的有效成分[J].华西药学杂志,2013,28(2):192
YANG Y,PAN Q,DING L,et al.Determination of active principles in Ligusticum chuanxiong by QNMR[J].West China J Pharm Sci,2013,28(2):192
[16] 成颜芬,吴亿晗,杨晓琴,等.川芎饮片标准汤剂的质量分析[J].中国实验方剂学杂志,2019,25(9):117
CHENG YF,WU YH,YANG XQ,et al.Quality analysis of standard decoction of Chuanxiong Rhizoma[J].Chin J Exp Tradit Med Form,2019,25(9):117
[17] YAN R,KO NL,LI SL,et al.Pharmacokinetics and metabolism of ligustilide,a major bioactive component in Rhizoma Chuanxiong,in the rat[J].Drug Metab Dispos,2008,36(2):400
Outlines

/