Objective: To establish a method for the simultaneous determination of paeoniflorin,prim-Oglucosylcimifugin, ferulic acid,5-O-methylvisammioside,baicalin,imperatorin,isoimperatorin,emodin and physcion in urticaria pills. Methods: HPLC was performed on the column of JADE-PAK ODS-AQ C18with the mobile phase of methanol-phosphoric acid at a flow rate of 1. 0 mL·min-1. The detection wavelength was 230 nm for paeoniflorin,254 nm for cimifugin,5-O-methylvisammioside,emodin and parietin,280 nm for baicalin and 300 nm for imperatorin and isoimperatorin,321 nm for ferulic acid,respectively. Column temperature was 25 ℃, and the volume injection was 10 μL. Results: Nine constituents showed good linear relationships within their own ranges（r≥0. 999）, and the average recoveries were 95. 2%-101. 0% with the RSDs of 1. 2%-2. 0%. The content ranges of paeoniflorin,prim-O-glucosylcimifugin,ferulic acid, 5-O-methylvisammioside, baicalin, imperatorin, isoimperatorin, emodin, physcion in 3 samples were 2. 014-2. 161,0. 205-0. 220, 0. 032-0. 038, 0. 241-0. 251, 0. 048-0. 050, 0. 785-0. 811,0. 042-0. 049,0. 095-0. 102,0. 037-0. 041 mg·g-1, respectively. Conclusion: This simple, accurate and reproducible method can be used for the quality control of urticaria pills.
LIN Hai-xia, REN Hua-zhong
. Simultaneous determination of nine constituents in urticaria pills by HPLC[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(4)
: 700
-704
.
DOI: 10.16155/j.0254-1793.2021.04.18
[1] 卫生部药品标准.中药成方制剂.第十册[S].1993:106
Drug Specifications Promulgated by the Ministry of Public Health,P R China.Prescription Preparation of Traditional Chinese Medicine.Vol 10[S].1993:106
[2] 张诚贤,徐陆忠,朱佳茜,等.高效液相色谱法测定荨麻疹丸中黄芩苷的含量[J].医药导报,2013,32(12):1649
ZHANG CX,XU LZ,ZHU JQ,et al.Content determination of baicalin in urticarial pill by HPLC[J].Her Med,2013,32(12):1649
[3] 蔡伊丽,周修森.HPLC 法测定荨麻疹丸中欧前胡素的含量[J].中国中医药现代远程教育,2014,12(24):157
CAI YL,ZHOU XS.Content determination of imperatorin in urticarial pills by HPLC[J].Chin Med Mod Dis Educ China,2014,12(24):157
[4] 雷琪,田蜜.HPLC 法同时测定荨麻疹丸中3种有效成分的含量[J].中国药房,2016,27(24):3437
LEI Q,TIAN M.Simultaneous determination of three components in urticarial pill by HPLC[J].China Pharm,2016,27(24):3437
[5] 肖小河,金城,鄢丹,等.中药大质量观及实践[J].中草药,2010,41(4):505
XIAO XH,JIN C,YAN D,et al.Proposition and practice on “integrative quality” in quality control for Chinese materia medica[J].Chin Tradit Herb Drugs,2010,41(4):505
[6] 杨冰,封亮,贾晓斌.基于“结构组分”特征的中药制剂质量评价策略[J].中草药,2019,50(17):4003
YANG B,FENG L,JIA XB.Quality evaluation strategy of Chinese materia medica preparation based on characteristics of“ structural composition of multi-components”[J].Chin Tradit Herb Drugs,2019,50(17):4003
[7] 汪小莉,刘晓,韩燕全,等.中药药效物质基础主要研究方法概述[J].中草药,2018,49(4):941
WANG XL,LIU X,HAN YQ,et al.Overview on main research method of effective material basis of Chinese materia medica[J].Chin Tradit Herb Drugs,2018,49(4):941
[8] 项丽玲,苗明三.中药质量生物学评价的思考[J].时珍国医国药,2020,31(6):1437
XIANG LL,MIAO MS.Consideration on biological evaluation of quality of Chinese materia medica[J].Lishizhen Med Mater Med Res,2020,31(6):1437
[9] 严红梅,陈小云,张振海,等.基于中药组分和“组分结构”理论的中药研究模式的探讨[J].中草药,2015,46(8):1103
YAN HM,CHEN XY,ZHANG ZH,et al.Discussion about research ideas of Chinese materia medica based on Chinese materia medica components and“ composition structure” theory[J].Chin Tradit Herb Drugs,2015,46(8):1103
[10] 陈振华,刘苏珍,周斌,等.浅谈中药质量标准现状与几种质量评价方法[J].时珍国医国药,2016,27(3):694
CHEN ZH,LIU SZ,ZHOU B,et al.Discussion on the present situation of quality standard of Chinese materia medica and quality evaluation methods[J].Lishizhen Med Mater Med Res,2016,27(3):694
