Objective: To establish a rapid analysis method of QuEChERS technology combined with gas chromatography-tandem mass spectrometry（GC-MS/MS）to detect 65 pesticide residues in Chuanxiong Rhizoma,and to screen 38 batches of ligusticum chuanxiong samples from different markets and different origins. Methods: Chuanxiong Rhizoma was treated with water and extracted with acetonitrile,and the QuEChERS package（containing anhydrous magnesium sulfate and anhydrous sodium acetate,etc.）was used for salting out. After the extraction solution was concentrated,internal standard triphenyl phosphate was added,and GC-MS/MS was used for detection and analysis. Chromatography-mass spectrometry conditions are as to Uows:Elastic quartz capillary column（30.0 m×0.25 mm×0.25 µm）DB17-MS was used,high purity helium was sued as carrier gas,injection volume was 1 μL,linear velocity control mode was used to control column velocity,the initial velocity of 1.3 mL·min-1.The inlet temperature was 240 ℃. Dynamic multi-reaction monitoring mode（MRM）was used. Ion source temperature was 200 ℃ and the interface temperature was 280 ℃. Results: All there a good linear relationship within certain content ranges for 65 pesticides,with the correlation coefficients（r）greater than 0.9987. Under the three addition levels,the recovery range were within 60% -140% for 93.85% of 65 pesticides,and for 92.31% of the pesticides the relative standard deviation was less than 15%. Thirty-eight batches of chuanxiong samples were tested and six pesticides were detected. The detection rate of chlorpyrifos was 81.58%. Conclusion: This method is simple,sensitive and accurate,which can be used for rapid screening and detection of various pesticide residues in Chuanxiong Rhizoma,and can provide technical support for the cultivation,production and quality control of Chuanxiong Rhizoma.
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