Objective: To determine 28 kinds of elements in pine needles of Cedrus deodara by inductively coupled plasma mass spectrometry（IPC-MS）and atomic fluorescence spectrometry（AFS）. Methods: These samples were digested by microwave in nitric acid water system,and mercury（Hg）in pine needles was determined by atomic fluorescence spectrometry,and 27 kinds of elements such as Li,Be,Na were determined by inductively coupled plasma mass spectrometry. Results: The linear relationship of 28 elements was good with r ≥ 0.9992. The RSD values of precision,repeatability and stability test were less than 4.0%,4.3% and 3.5% respectively. The recovery rates were between 88.6% and 110.6%,and the RSD values were all less than 4.9%. In 15 batches of samples,the contents of K,Mg,Ca,Na,Al,Fe,Sr,Mn,Zn and Ba were relatively high. Referring to the content limit of heavy metals and harmful elements in traditional Chinese medicine of Pharmacopoeia People’s Republic of China（2020）,it was found that the contents of heavy metal elements such as Pb,Cd,As,Hg and Cu did not exceed the limit. Six principal components were obtained by principal component analysis,and the cumulative variance contribution rate was 88.65%. It was indicated that Cs,V,Fe,Al,Cr,Se,Co,Tl,Be,As,Ca and K were the characteristic elements of the pine needles from Cedrus deodara. Conclusion: The method of ICP-MS and AFS for the determination of 28 elements in pine needles of Cedrus deodara has the characteristics of high accuracy and sensitivity. This study has certain potential and value for the quality control,further research and development of the pine needles from Cedrus deodara.
ZHANG Guo-qiang, LI Yun, LU Yan-mei, LI Wan-yu, SHI Xiao-feng, CHENG Xian-long
. Determination of 28 elements in pine needles of Cedrus deodara by super microwave digestion-ICP-MS/AFS*[J]. Chinese Journal of Pharmaceutical Analysis, 2021
, 41(6)
: 1046
-1053
.
DOI: 10.16155/j.0254-1793.2021.06.16
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