Objective: To establish high-performance liquid chromatography method using cyclodextrin as a mobile phase additive for the determination of ursolic acid and oleanolic acid in Corni Fructus, providing a basis for quality control of Corni Fructus. Methods: Molecular design and response surface method were used to optimize the determination method of ursolic acid and oleanolic acid in Corni Fructus. Agilent Eclipse XDB C18 column (150 mm×4.6 mm, 5 μm) was adopted as the stationary phase, the mobile phase was 3 mmol·L-1 γ-cyclodextrin solution (contained 0.05% phosphoric acid)-acetonitrile (60∶40), the flow rate was 1.0 mL·min-1, the column temperature was 25 ℃ and the detecting wavelength was 210 nm. Results: The resolution of chromatographic peaks of ursolic acid and oleanolic acid was 3.81. Linearities were good in the range of 400-4 000 ng for ursolic acid and in the range of 200-2 000 ng for oleanolic acid. The RSDs for precision, reproducibility, and stability were less than 1.4%, 1.8%, and 1.7%, respectively. The recovery rates were 98.5% (RSD=1.7%) and 99.1% (RSD=2.0%). The contents of ursolic acid in three batches of Corni Fructus were 2.66 mg·g-1, 2.35 mg·g-1 and 2.91 mg·g-1, and those of oleanolic acid were 0.83 mg·g-1, 0.78 mg·g-1and 1.12 mg·g-1, respectively. Conclusion: This method is simple, accurate, reliable, and environmental friendly, and can be used as a quality control for Corni Fructus.
YANG Ruo-meng, TIAN Jun, LI Yun-zhe
. Optimization of HPLC with cyclodextrin as the mobile phase additive for the determination of ursolic acid and oleanolic acid in Corni Fructus by molecular design and response surface method*[J]. Chinese Journal of Pharmaceutical Analysis, 2024
, 44(7)
: 1154
-1160
.
DOI: 10.16155/j.0254-1793.2023-0789
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