Application of metabolomics approach to study on chemical constituents in rhizomes and leaves of <i>Polygonatum sibiricum</i> from Shaanxi Province based on UPLC-Q-Orbitrap HRMS<sup>*</sup>

SONG Yi-jun, GUO Tao, LIANG Yu-ying, GUAN Geng-chen, GAO Yang-li

doi:10.16155/j.0254-1793.2024-0015

Chinese Journal of Pharmaceutical Analysis >

2024 , Vol. 44 >Issue 8: 1293 - 1302

DOI: https://doi.org/10.16155/j.0254-1793.2024-0015

Ingredient Analysis

Application of metabolomics approach to study on chemical constituents in rhizomes and leaves of Polygonatum sibiricum from Shaanxi Province based on UPLC-Q-Orbitrap HRMS^*

Expand

1. College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, Chinia;
2. Shaanxi Engineering Technology Research Center of Prepared Slices, Xianyang 712046, China;
3. No. 986 Hospital of PLAAF, Xi'an 710054, China

Received date: 2024-01-09

Online published: 2024-10-16

Fold

Abstract

Objective: To analyze the difference of chemical constituents in rhizomes and leaves of Polygonatum sibiricum from Shaanxi Province. Methods: Ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap HRMS) was developed to determine the chemical constituents in rhizomes and leaves of Polygonatum sibiricum from Shaanxi Province. The data were analyzed by principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). The structures of chemical markers in rhizomes and leaves of Polygonatum sibiricum were identified based on accurate primary mass spectrometry and secondary mass spectrometry fragment ion, combined with the reference map, software database searching and related literature. Results: A total of 45 compounds were identified, there were 26 chemical ingredients with significant differences distinguished by the method of OPLS-DA, including 10 amino acids, 6 flavonoids, 3 organic acids, 2 saccharides, 1coumarin and 4 alkaloids. Conclusion: The chemical markers of amino acids, organic acids and alkaloids are mainly distributed in rhizomes, and the chemical markers of flavonoids are mainly concentrated in leaves part of the plant. It is suggested that the potential multiple utilization of rhizomes and leaves of Polygonatum sibiricum from Shaanxi Province.

Key words： Polygonati Rhizoma; rhizomes; leaves; plant metabolomics; potential marker; principal component analysis (PCA); orthogonal partial least squares discriminant analysis (OPLS-DA); UPLC-Q-Orbitrap HRMS

Cite this article

SONG Yi-jun, GUO Tao, LIANG Yu-ying, GUAN Geng-chen, GAO Yang-li . Application of metabolomics approach to study on chemical constituents in rhizomes and leaves of Polygonatum sibiricum from Shaanxi Province based on UPLC-Q-Orbitrap HRMS^*[J]. Chinese Journal of Pharmaceutical Analysis, 2024 , 44(8) : 1293 -1302 . DOI: 10.16155/j.0254-1793.2024-0015

References

[1] 钟凌云. 中药炮制学[M]. 北京: 中国中医药出版社, 2021: 313
ZHONG LY. Processing of Chinese Materia Medica[M]. Beijing: China Press of Traditional Chinese Medicine, 2021: 313
[2] 曲寿河, 程喜乐, 潘英妮, 等. 黄精产地加工及炮制方法的历史沿革[J]. 沈阳药科大学学报, 2020, 37(4): 379
QU SH, CHENG XL, PAN YN, et al. The history of origin processing and processing methods of Polygonati Rhizoma[J]. J Shenyang Pharm Univ, 2020, 37(4): 379
[3] 张娇, 王元忠, 杨维泽, 等. 黄精属植物化学成分及药理活性研究进展[J]. 中国中药杂志, 2019, 44(10): 1989
ZHANG J, WANG YZ, YANG WZ, et al. Research progress in chemical constituents in plants of Polygonatum and their pharmacological effects[J]. China J Chin Mater Med, 2019, 44(10): 1989
[4] 葛洪. 抱朴子内篇[M]. 北京:中国中医药出版社, 1997: 99
GE H. Baopuzi Neipian[M]. Beijing: China Press of Traditional Chinese Medicine, 1997: 99
[5] 陶弘景. 本草经集注[M]. 北京: 人民卫生出版社, 1994:198
TAO HJ. Bencaojing Jizhu[M]. Beijing: People's Medical Publishing House, 1994: 198
[6] 孟诜. 食疗本草[M]. 北京: 人民卫生出版社, 1984:2
MENG S. Shiliao Bencao[M]. Beijing: People's Medical Publishing House, 1984: 2
[7] SONG YJ, GUO T, LIU SJ, et al. Identification of Polygonati Rhizoma in three species and from different producing areas of each species using HS-GC-IMS[J]. LWT-Food Sci Technol,2022(172): 114142
[8] 宋艺君, 郭涛, 高阳丽, 等. GC-IMS法比较黄精不同炮制品特征气味物质差异[J]. 中国药学杂志, 2022, 57(16): 1329
SONG YJ, GUO T, GAO YL, et al. Using GC-IMS to compare the difference of specific odor substance of Polygonati Rhizoma in different processed products[J]. Chin Phram J, 2022, 57(16): 1329
[9] 石双慧, 王梦琳, 魏晓彤, 等. AHP-熵权法结合Box-Behnken设计-响应面法优选黄精酒制工艺及其炮制前后药效对比研究[J]. 中草药, 2023, 54(14):4467
SHI SH,WANG ML, WEI XT, et al. Optimization of processing technology of wine-processed Polygonati Rhizoma by combination of Box-Behnken design-response surface method and AHP-entropy weight method and comparison of efficacy before and after processing[J]. Chin Tradit Herb Drugs, 2023, 54(14):4467
[10] 齐彦爽, 李静, 郭冬, 等. 基于核磁共振代谢组学技术的款冬茎和叶的化学成分比较[J]. 中国药学杂志, 2019, 54(8): 608
QI YS, LI J, GUO D, et al. Chemical comparison of the stems and leaves of Tussilago farfara L. using NMR-based metabolomics[J]. Chin Phram J, 2019, 54(8): 608
[11] 陈俊可, 曾锐. 基于UPLC-ESI-HRMSⁿ的代谢组学技术对粗茎秦艽不同部位化学成分研究[J]. 中草药, 2018, 49(10): 2328
CHEN JK, ZENG R. Application of metabolimics approach to study on chemical constituents in different parts of Gentiana crasicaulis based on UPLC-ESI-HRMSⁿ[J]. Chin Tradit Herb Drugs, 2018, 49(10): 2328
[12] LI X, WANG P, TONG YP, et al. UHPLC-Q-Exactive Orbitrap MS/MS based untargeted metabolomics and molecular networking reveal the differential chemical constituents of the bulbs and flowers of Fritillaria thunbergii[J]. Molecules, 2022, 27(20): 6944. https://doi.org/10.3390/molecules27206944
[13] WANG F, CHEN L, CHEN HP, et al. Discovery of the key active compounds in Citri Reticulatae Pericarpium (Citrus reticulate “Chachi”) and their therapeutic potential for the treatment of COVID-19 based on comparative metabolomics and network pharmacology[J]. Front Pharmacol, 2022,13:1048926.doi: 10.3389/fphar.2022.1048926
[14] 庞溢媛, 薛立英, 郑艳红, 等. 基于UHPLC-MS/MS代谢组学技术的不同采收期黄芩质量比较研究[J]. 药学学报, 2017, 52(12): 1903
PANG YY, XUE LY, ZHENG YH, et al. Comparative study on quality of Scutellaria baicalensis Georgi in different harvest periods using UHPLC-MS/MS metabolomics technology[J]. Acta Pharm Sin, 2017, 52(12): 1903
[15] 李震宇, 崔伊凡, 秦雪梅. 中药材质量评价的挑战与代谢组学应用于中药材质量评价的研究进展[J]. 中草药, 2018, 49(10): 2221
LI ZY, CUI YF, QIN XM. Challenge of quality evaluation of traditional Chinese medicinal materials and application progress on metabolomic approach in its quality valuation[J]. Chin Tradit Herb Drugs, 2018, 49(10): 2221
[16] SALEM MA, EZZAT SM, GIAVALISCO P, et al. Application of a comprehensive metabolomics approach for the selection of flaxseed varieties with the highest nutritional and medicinal attributes[J]. J Food Drug Anal, 2021, 29(2): 214
[17] 薛淑娟, 陈随清. 基于代谢组学分析不同蒸制次数熟地黄中糖类成分的变化规律[J]. 中国实验方剂学杂志, 2019, 24(22): 1
XUE SJ, CHEN SQ. Analysis on change rule of carbohydrates in Rehmanniae Radix Praeparata with different processing times based on metabolomics[J]. Chin J Exp Tradit Med Form, 2019, 24(22): 1
[18] WANG SR, WEI SJ, ZHU YM, et al. Comparative investigation of the differences in chemical compounds between raw and processed Mume Fructus using plant metabolomics combined with chemometrics methods[J]. Molecules, 2022, 27: 6344. https://doi.org/10.3390/molecu les27196344
[19] 华愉教, 侯娅, 王胜男, 等. 基于¹H-NMR代谢组学技术的野生与栽培太子参化学成分分析[J]. 中国药学杂志, 2017, 52(4): 24
HUA YJ, HOU Y, WANG SN, et al. ¹H-NMR based metabolomic analysis of chemical compositions in cultivated and wild Pseudostellariae Radix[J]. Chin Phram J, 2017, 52(4): 24
[20] 王立会, 张娜, 俞磊明, 等. 基于UPLC-Q TOF-MS^E植物代谢组学的不同产地黄芪差异性研究[J]. 中国药师, 2022, 25(12): 2165
WANG LH, ZHANG N, YU LM, et al. Study on the diversities of Astragali Radix from different growing regions based on UPLC-Q TOF-MS^Eplants metabolomics[J]. China Pharm, 2022, 25(12): 2165
[21] GHIRARDO A, FOCHI V, LANGE B, et al. Metabolomic adjustments in the orchid mycorhizal fungus Tulasnella calospora during symbiosis with Serapias womeracea[J]. New phytol, 2020, 228(6):1939
[22] 赵文莉, 赵晔, Yiider Tseng. 黄精药理作用研究进展[J]. 中草药, 2018, 49(18): 4439
ZHAO WL, ZHAO Y, YIIDER T. Research progress on pharmacological effects of Polygonati Rhizoma[J]. Chin Tradit Herb Drugs, 2018, 49(18): 4439
[23] 任洪民, 邓亚羚, 张金莲, 等. 药用黄精炮制的历史沿革、化学成分及药理作用研究进展[J]. 中国中药杂志, 2020, 45(17): 4163
REN HM, DENG YL, ZHANG JL, et al. Research progress on processing history evolution,chemical components and pharmacological effects of Polygonati Rhizoma[J]. China J Chin Mater Med, 2020, 45(17): 4163
[24] 刘爽,胡舒婷,贾巧君, 等. 黄精的化学组成及药理作用研究进展[J]. 天然产物研究与开发, 2021, 33(10): 1783
LIU S, HU ST, JIA QJ, et al. Advances in chemical constituents and pharmacological effects of Polygonati Rhizoma[J]. Nat Prod Res Dev, 2021, 33(10): 1783
[25] 姜程曦,张铁军,陈常青, 等. 黄精的研究进展及其质量标志物的预测分析[J]. 中草药, 2017, 48(1): 1
JIANG CX, ZHANG TJ, CHEN CQ, et al. Research progress in Polygonati Rhizoma and predictive analysis on Q-marker[J]. Chin Tradit Herb Drugs, 2017, 48(1): 1
[26] 薛妙,郭凯丽,袁盼盼,等. 酒黄精总黄酮提取工艺及其抗氧化作用研究[J]. 中南药学, 2023, 21(2): 351
XUE M,GUO KL, YUAN PP, et al. Extraction optimization and antioxidant activities of total flavonoid from alcoholic Polygonatum[J]. Cent South Pharm,2023, 21(2): 351
[27] 杨显辉, 代培春, 曾磊, 等. 滇黄精总黄酮抗运动疲劳作用研究[J]. 现代食品, 2019(8): 134.doi:10.16736/j.cnki.cn41-1434/ts.2019.08.038
YANG XH, DAI PC, ZENG L, et al. Anti-fatigue effect and mechanism of flavonoids from polygonatum Rhizoma[J]. Mod Food, 2019(8):134.doi:10.16736/j.cnki.cn41-1434/ts.2019.08.038
[28] 陶爱恩, 张晓灿, 杜泽飞, 等. 黄精属植物中黄酮类化合物及其药理活性研究进展[J]. 中草药, 2018, 49(9): 2163
TAO AE, ZHANG XC, DU ZF, et al. Research progress on flavonoids in plants of Polygonatum Mill. and their pharmacological activities[J]. Chin Tradit Herb Drugs, 2018, 49(9): 2163

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References