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Study on chemical composition difference between ancient and modern Baihe Dihuang decoction based on multi-component analysis*

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  • 1. College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China;
    2. Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-medicine, Henan Province, Henan University of Chinese Medicine, Zhengzhou 450046, China;
    3. Collaborative Innovation Center for Respiratory Diseases, Henan University of Chinese Medicine, Zhengzhou 450046, China

Received date: 2024-02-27

  Online published: 2024-10-17

Abstract

Objective: To study the difference of chemical composition between ancient and modern clinical prescriptions of Baihe Dihuang decoction by multi-component analysis. Methods: The polysaccharide extract of Baihe Dihuang decoction was determined by anthrone-sulfuric acid-ultraviolet spectrophotometer under 580 nm ultraviolet light. Six monosaccharides and oligosaccharides in the ancient and modern Baihe Dihuang decoction were determined by UPLC-CAD. An Agilent InfinityLab Poroshell 120 HILIC-Z (3.0×100 mm,2.7 μm) column was used. The mobile phase was consisted of A 0.2% ammonia in acetonitrile and 0.2% ammonia in water with gradient elution. The flow rate was 0.8 mL·min-1, the column temperature 35 ℃ and the CAD detector nebulizer temperature was 70 ℃. Data acquisition frequency was 10 Hz and the filter was 1.05 s. The injection volumn was 2 μL. The non-polar components were qualitatively and quantitatively analyzed by high performance liquid-quadrupole tandem time-of-flight mass spectrometry (UPLC-Q TOF MS/MS) and high performance liquid-phase tandem triple quadrupole mass spectrometry (UPLC-QQQ MS), respectively. The differential components of ancient and modern Baihe Dihuang Decoction were analyzed and 9 components were determined. Results: The linear relationship of 6 mono-oligosaccharides/polysaccharides and 9 differential components was good (r>0.999). The average recoveries ranged from 97.7% to 104.9% with RSDs≤3.1%. Precision, repeatability and stability met the requirements. The results showed that the conents of all the analytes in ancient and modern Baihe Dihuang decoction were significantly different (P<0.05). Conclusion: The methods used in this experiment are accurate, sensitive, stable and reproducible, which can provide reference for the quality control and pharmacodynamic material basic research of Baihe Dihuang Decoction.

Cite this article

JIA Fu-kang, FENG Jian-hui, WANG Bi-ying, LIU Rong-wei, CHEN Sui-qing, FU Yu . Study on chemical composition difference between ancient and modern Baihe Dihuang decoction based on multi-component analysis*[J]. Chinese Journal of Pharmaceutical Analysis, 2024 , 44(9) : 1535 -1550 . DOI: 10.16155/j.0254-1793.2024-0123

References

[1] 张仲景.金匮要略[M]. 北京:中国古籍出版社, 2018:125
ZHANG ZJ. Synopsis of the Golden Chamber[M]. Beijing: Ancient Books Publishing House, 2018: 125
[2] 佚名. 神农本草经 [M]. 北京:人民卫生出版社,1956
YI M. Sheng Nong's Herbal Classic [M]. Beijing: People's Health Publishing House, 1956
[3] 李艳, 郭晖, 苗明三. 中药鲜用的药理作用及临床应用分析 [J]. 中华中医药杂志, 2019, 34(12): 5691
LI Y, GUO H, MIAO MS. Pharmacological effects and clinical application analysis on fresh use of traditional Chinese medicine [J]. Chin J Tradit Chin Med Pharm, 2019, 34(12): 5691
[4] 宋如珺, 韩赟, 胡芳, 等. 温病医著中鲜药的应用规律研究 [J]. 北京中医药, 2022, 41(1): 54
SONG RJ, HAN Y, HU F, et al. Study on the application of fresh herbal medicines based on literature about febrile diseases [J]. Beijing J Tradit Chin Med Pharm, 2022, 41(1): 54
[5] 王梦溪,吴启南,乐巍,等. 中药鲜药的应用与现代研究 [J]. 中草药, 2015, 46(20): 3125
WANG MX, WU QN, YUE W, et al. Application and modern research on fresh herbs of Chinese materia medica [J]. Chin Tradit Herb Drugs, 2015, 46(20): 3125
[6] 高宇. 百合地黄汤治疗脑卒中术后昼夜节律睡眠-觉醒障碍心肺阴虚证的临床观察 [D]. 长沙:湖南中医药大学, 2022
GAO Y, Clinical Observation of Baihe Dihuang Decoction in Treating Cardiopulmonary Yin Deficiency Syndrome of Circadian Rhythm Sleep-Wake Disorder After Stroke [D]. Changsha:Hunan University Traditional Chinese Medicine, 2022
[7] 褚雪菲, 刘道龙, 周军怀, 等. 四逆散合百合地黄汤加减干预焦虑性失眠的临床观察 [J]. 中国中医基础医学杂志, 2021, 27(8): 1295
CHU XF, LIU DL, ZHOU JH, et al. Clinical observation of Sini San combined with Baihe Dihuang decoction in the intervention of anxiety insomnia [J]. Chin J Basic Med Tradit Chin Med, 2021, 27(8): 1295
[8] 刘伟群, 吴松鹰.百合地黄汤治疗神志病研究概况 [J]. 中医药临床杂志, 2019, 31(10): 1816
LIU WQ, WU SY. Research progress of Baihe Dihuang decoction in the treatment of psychiatric diseases [J]. Clin J Tradit Chin Med, 2019, 31(10): 1816
[9] LI C, CAO H, HUAN Y, et al. Berberine combined with stachyose improves glycometabolism and gut microbiota through regulating colonic microRNA and gene expression in diabetic rats[J]. Life Sci, 2021, 284: 119928
[10] ZHANG B, ZHOU N, ZHANG Z, et al. Study on the neuroprotective effects of eight iridoid components using cell metabolomics [J]. Molecules, 2024, 29(7): 1497
[11] ZHANG Y, WU MX, LI HM, et al. Potential benefits of Rehmanniae Radix after ancient rice-steaming process in promotion of antioxidant activity in rats' health [J]. Food Sci Nutr, 2023, 11(9): 5532
[12] 彭修娟, 杨新杰, 陈衍斌, 等. 基于中药整合药理学平台的百合地黄汤治疗抑郁症的作用机制研究 [J]. 中国中药杂志, 2018, 43(7): 1338
PENG XJ, YANG XJ, CHEN YB, et al. Action mechanism of Baihe Dihuang decoction on depression based on integrative pharmacology of traditional Chinese medicine [J]. China J Chin Mater Med,2018, 43(7): 1338
[13] 但济修, 周永全, 詹常森.百合地黄汤处方剂量研究 [J]. 中成药, 2023, 45(10): 3402
DAN JX, ZHOU YQ, ZHAN CS. Study on prescription dose of Baihe Dihuang decoction [J]. Chin Tradit Pat Med, 2023, 45(10): 3402
[14] 王碧莹, 付钰, 张欣亚, 等. 指纹图谱联合多元统计分析的百合地黄汤物质基准质量评价及量值传递规律研究 [J]. 中医学报, 2024, 39(1): 1674
WANG BY, FU Y, ZHANG XY, et al. Quality evaluation and quantity transfer rule of Baihe Dihuang decoction combining fingerprint and multivariate statistical analysis [J]. J Acta Chin Med, 2024, 39(1): 1674
[15] 钱艳艳. 地黄不同炮制品糖类成分理化特性及高压蒸制条件优化研究 [D]. 郑州: 河南农业大学, 2022
QIAN YY, Study on Physicochemical Properties of Saccharides from Different Processed Products of Rehmannia glutinosa and Optimization of High Pressure Steaming Conditions [D]. Zhengzhou:Henan Agriculture University, 2022
[16] MONTILLA A, OLANO A, MARTINEZ-VILLALUENGA C, et al. Study of influential factors on oligosaccharide formation by fructosyltransferase activity duringstachyose hydrolysis by pectinex ultra SP-L [J]. J Agric Food Chem, 2011, 59(19): 10705
[17] ZHANG W, DU F,WANG L, et al. Hydrolysis of oligosaccharidesby a thermostable α-galactosidase from Termitomyces eurrhizus [J]. Int J Mol Sci, 2015, 16(12): 29226
[18] 徐双双, 吴思佳, 卢文旭, 等. HPLC-CAD法同时测定铁皮石斛中单糖和寡糖类成分的含量 [J]. 药物分析杂志, 2023, 43(8): 1302
XU SS, WU SJ, LU WX, et al. Simultaneous determination of monosaccharides and oligosaccharides in Dendrobii officinalis caulis by HPLC-CAD [J]. Chin J Pharm Anal, 2023, 43(8): 1302
[19] 陈蓉, 马冬阳, 郭彬,等. HPLC-CAD法结合化学计量学同时测定甘草中多种糖类 [J]. 中国药师, 2021, 24(10): 1932
CHEN R,MA DY,GUO B, et al. Simultaneous quantification of saccharides in Glyeyrrhizae Radix et Rhizoma by HPLC. CAD combined with chemometrics [J]. China Pharm, 2021, 24(10): 1932
[20] 贾秀梅,张振凌. HPLC-ELSD法测定鲜地黄及其炮制加工品中水苏糖的含量 [J]. 新疆中医药, 2022, 40(5): 53
JIA XM, ZHANG ZL. Determination of content of stachyose in fresh Rehmannia root and its processed products by HPLC-ELSD [J]. J Xinjiang Tradit Chin Med Pharm, 2022, 40(5): 53
[21] 田家屹,马芳,韩玲玉. 基于红外光谱的鲜地黄、生地黄和熟地黄成分差异分析 [J]. 光谱学与光谱分析, 2022,42(10): 3203
TIAN JY, MA F, HAN LY. Analysis of composition differences of fresh Radix Rehmanniae, crude Radix Rehmanniae and processed Radix Rehmanniae using infrared spectroscopy [J]. Specyrosc Spect Anal, 2022, 42(10): 3203
[22] 赵婧含,李雪,吴文轩,等.熟地黄的化学成分及药理作用研究进展 [J]. 中医药学报, 2023, 51(6): 110
ZHAO JH, LI X, WU WX, et al. Research progress on chemical constituents and pharmacological effects of Radix Rehmanniae Praeparata [J]. Acta Chin Med,2023, 51(6): 110
[23] 王新民,黄小婉,杨影影,等. 怀地黄不同炮制品中5种成分差异的研究 [J]. 现代牧业,2023,7(3): 8
WANG XM, HUANG XW, YANG YY, et al. Study on the difference of five components in different processed products of Rehmanniae Radix [J]. Mod Anim Husb, 2023, 7(3): 8
[24] 陈佳妮,罗耀华,孔慧,等. 热激处理对鲜切百合鳞茎片贮藏品质的影响 [J]. 食品科学,2024,45(1):1
CHEN JN, LUO YH, KONG H, et al. Effect of heat shock treatment on the storage quality of fresh-cut lily bulb slices [J]. Food Sci, 2024, 45(1):1
[25] LIU F, CHANG X, HU X, et al. Effect of thermal processing on phenolic profiles and antioxidant activities in Castanea mollissima [J]. Int J Food Sci Technol, 2017, 52(2): 439
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