Standard Deliberation

Comparative study of different species of Epimedium based on HPLC fingerprint and multi-index assay

Expand
  • 1. Gansu University of Traditional Chinese Medicine, Lanzhou 730030, China;
    2. National Key Laboratory of Quality Control of Chinese Medicinal Materials and Decoction Pieces, Gansu Provincial Engineering Laboratory of Chinese and Tibetan Medicine Inspection and Testing Technology, Gansu Institute of Pharmaceutical Inspection and Control, Lanzhou 730030, China

Revised date: 2024-06-25

  Online published: 2024-10-17

Abstract

Objective: To investigate the source and quality of commercially available Epimedium based on HPLC fingerprint and multi-index assay, so as to provide reference for its quality evaluation and resource development. Methods: Forty batches of commercially available epimedium were collected. Fingerprint analysis and determination of the total flavonoid glycosides were performed by HPLC method. The total flavonoid glycosides were determined by UV method. And the contents of their extracts were determined. Stoichiometric analysis was employed to perform the comprehensive quality evaluation of different medicinal materials of Epimedium. Results: There were 9 different plant sources of Epimedium in the market. The five authentic Epimedium were of good quality, and the local varieties were coarse Epimedium. The contents were high in Epimedium from Hunan, while the contents were low in Epimedium from Qianling and Sichuan. The comprehensive quality ranking of Epimedium indicated that Epimedium Fletchum and Epimedium Fletchum ranked first by the principal component analysis method. The ranking of the same variety varied greatly with different habitats, and the Qianling Epimedium and adulterated Epimedium ranked last. The results of cluster analysis and principal component analysis were basically consistent. The cluster analysis showed distinguishing significance in plant sources, habitats, processing methods and contents. Based on the common pattern of E. brevicornu, a total of 8 common peaks were identified for 9 species of E. Brevicornu, and their similarities ranged from 0.066 to 0.979. Differences were observed among different species. Conclusion: Both the fingerprint similarity and the chemical model of content determination can be used to evaluate the species and quality of Epimedium.

Cite this article

LIU Xin, LIN Rui-li, NI Lin, SONG Ping-shun, YANG Ling-xia . Comparative study of different species of Epimedium based on HPLC fingerprint and multi-index assay[J]. Chinese Journal of Pharmaceutical Analysis, 2024 , 44(9) : 1613 -1623 . DOI: 10.16155/j.0254-1793.2023-0419

References

[1] 中华人民共和国药典2020年版. 一部[S]. 2020: 340
ChP 2020.Vol Ⅰ[S]. 2020:340
[2] 龚华乾, 高敏, 柴艺汇, 等. 淫羊藿化学成分与药理作用研究进展[J]. 湖北民族大学学报, 2021, 38(4): 75
GONG HQ, GAO M, CHAI YH, et al. Advances in chemical constituentsand pharmacological effects of Epimedium [J]. J Hubei Univ Nat, 2021, 38(4): 75
[3] CAO LH, QIAO JY, HUANG HY, et al. PI3K-AKT signaling activation and icariin: the potential effecs on the perimenopausal depression-like rat model[J]. Molecules, 2019, 24(20): 3700
[4] JIN J, WANG H, HUA X, et al. An outline for the pharmacological effectof icariin in the nervous system[J]. Eur J Pharmacol, 2019, 842: 20
[5] ZHAO H, SHAN Y, MA Z, et al. A network pharmacology approach to explore active compounds and pharmacological mechanisms of epimedium fortreatment of premature ovarian insufficiency[J]. Drug Des Devel Ther, 2019, 13: 2997
[6] 乔韬, 许栋, 陈昳冰, 等. 基于文献计量的淫羊藿苷研究热点和未来趋势分析[J]. 中草药, 2020, 52(23): 7293
QIAO T, XU D, CHEN YB, et al. Research hotspots and future trend of icariin based on bibliometric analysis [J]. Chin Herb Med, 2020, 52(23): 7293
[7] 谢宗万. 中药材品种论述,中册[M]. 上海:上海科学技术出版社, 1984: 241
XIE ZW. Discussion on Varieties of Chinese Medicinal Materials, Volume [M]. Shanghai: Shanghai Science and Technology Press, 1984:241
[8] 郭宝林, 肖培根. 中药淫羊霍主要种类评述[J]. 中国中药杂志, 2003, 28(4): 303
GUO BL, XIAO PG. Review on main species of Herba herba [J]. Chin J Tradit Chin Med, 2003, 28(4): 303
[9] 林瑞超. 中国药材标准名录[M]. 北京:科学出版社, 2011:468,561
LIN RC. Standard List of Chinese Medicinal Materials [M]. Beijing: Science Press, 2011:468, 561
[10] 裴利宽, 郭宝林. 近10年淫羊霍药材及饮片研究进展[J]. 中国中药杂志, 2007, 32(6): 466
PEI LK, GUO BL. Research progress of Chinese herbal medicine and decoction pieces in recent 10 years [J]. Chin J Tradit Chin Med, 2007, 32(6): 466
[11] 裴利宽, 郭宝林, 黄文华. 中药淫羊霍主要种类药材质量的系统研究[J]. 中国中药杂志, 2007, 32(21):2217
PEI LK, GUO BL, HUANG WH. Systematic study on the quality of main species of Herba herba[J]. Chin J Tradit Chin Med, 2007, 32(21):2217
[12] 成丽媛, 霍香香, 杨莉, 等. UPLC法测定不同基源淫羊藿中11种有效成分的含量[J]. 天津中医药大学学报, 2022, 41(2): 237
CHENG LY, HUO XX, YANG L, et al. Determination of 11 active components in Epimedium from different base by UPLC [J]. J Tianjin Univ Tradit Chin Med, 2022, 41(2): 237
[13] 陈肖家, 张庆文, 季晖, 等. 紫外分光光度法和高效液相色谱法测定淫羊藿总黄酮含量的比较研究[J]. 药物分析杂志, 2007,17(5): 625
CHEN XJ, ZHANG QW, JI H, et al. Comparative study on the content of total flavonoids in Epimedium by ultraviolet spectrophotometry and high performance liquid chromatography [J]. Chin J Pharm Anal, 2007,17(5): 625
[14] 石慧君, 刘少雄, 徐燕琴, 等. 湖南省淫羊藿属植物种质分布及质量评价[J]. 中国实验方剂学杂志, 2016, 22(24): 26
SHI HJ, LIU SX, XU YQ, et al. Germplasm distribution and quality evaluation of Epimedium in Hunan Province [J]. Chin J Exper Formul, 2016, 22(24): 26
[15] 何顺志, 王悦云, 徐文芬,等. 贵州淫羊藿药材种质资源的研究[J]. 种子, 2011, 30(5): 69
HE SZ, WANG YY, XU WF, et al. Study on germplasm resources of Epimedium in Guizhou[J]. Seed, 2011, 30(5): 69
[16] 刘鑫, 宋平顺. 基于HPLC含量测定法结合化学统计学对甘肃地产淫羊藿的质量评价体系研究[J]. 中医药信息, 2023, 40(8): 29
LIU X, SONG PS. Study on quality evaluation system of real estate Epimedium in Gansu Province based on HPLC content determination combined with chemical statistics [J]. Inf Chin Med, 2019,40(8):29
[17] 李仁清, 田淑云, 李风琴, 等. 4种淫羊藿产量与质量的动态变化及最佳采收期研究[J]. 时珍国医国药, 2022, 33(6): 1456
LI RQ, TIAN SY, LI FQ, et al. Study on dynamic changes of yield and quality of four Epimedium species and optimal harvesting time [J]. Lishizhen Med Mater Med Res, 2022, 33(6): 1456
[18] 黄世琼, 张永东, 郑浩文, 等. 不同地区淫羊藿药材质量分析[J]. 亚太传统医药, 2020, 16(8): 55
HUANG SQ, ZHANG YD, ZHENG HW, et al. Quality analysis of Epimedium in different regions [J]. Asian-Pac Tradit Med, 2020,16(8): 55
[19] 王常顺, 段吉平, 康帅, 等. 淫羊藿药材及饮片质量分析与标准制定[J]. 中国现代中药, 2022, 22(7): 1006
WANG CS, DUAN JP, KANG S, et al. Quality analysis and standard formulation of Herba epimedium [J]. Chin J Mod Chin Med, 2022, 22(7): 1006
[20] 周政政, 罗建光, 郭宝林, 等. 不同产地主要淫羊藿品种中活性黄酮类成分分析及资源研究[J]. 中国药科大学学报, 2010, 41(2): 146
ZHOU ZZ, LUO JG, GUO BL, et al. Analysis of active flavonoids in main Epimedium varieties from different regions and study on their resources [J]. J China Pharm Univ, 2010, 41(2): 146
Outlines

/