Objective: To develop a method for the determination of meropenem and vaborbactam for injection by HPLC. Methods: The chromatography was performed on Shimadzu InertSustain C18(150 mm×4.6 mm, 5 μm)column with acetonitrile-0.02 mol·L-1 sodium dihydrogen phosphate solution (adjust pH to 2.8 with phosphoric acid)(10∶90) as a mobile phase, the flow rate was 1.0 mL·min-1, the detection wavelength was 210 nm, and the sample volume was 10 μL. A method for the determination of meropenem and vaborbactam for injection was established under the chromatographic conditions. Results: In this method, the linearities of meropenem and vaborbactam were good, and the linearity ranges were 21.40-214.0 μg·mL-1 and 19.83-198.3 μg·mL-1. Meropenem and vaborbactam had good precision with RSD of 0.31% and 0.16%, respectively. Within 24 h, meropenem and vaborbactam had good stabilities at 4 ℃, RSDs were 0.31% and 0.16%, respectively. The average recoveries of meropenem and vaborbactam were 101.0%(n=9) and 98.4%(n=9) (RSD＜2.0%). The contents of meropenem and vaborbactam were 405.8 mg·g-1 and 399.1 mg·g-1, respectively. Conclusion: The method is accurate, simple and rapid, and can be used for the determination of meropenem and vaborbactam for injection.
WANG Jing
,
XU Shuo
,
WANG Yue
,
GUO Si-rui
,
XU Wen-feng
,
JIN Peng-fei
. Determination of meropenem and vaborbactam for injection by high performance liquid chromatography*[J]. Chinese Journal of Pharmaceutical Analysis, 2024
, 44(10)
: 1679
-1684
.
DOI: 10.16155/j.0254-1793.2024-0175
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