目的: 调查市售淫羊藿来源,采用HPLC指纹图谱及多指标成分含量测定,对市售淫羊藿药材进行质量研究,为其质量评价和资源开发提供参考。方法: 收集到40批市售淫羊藿药材,采用HPLC指纹图谱分析,HPLC测定总黄酮醇苷、UV法测定总黄酮含量,并测定浸出物含量,应用化学计量学分析方法,完成不同淫羊藿药材的综合质量评价。结果: 市售淫羊藿有9种不同植物来源;5种正品淫羊藿质量良好,地方品种以粗毛淫羊藿、湖南淫羊藿含量较高,而黔岭淫羊藿、四川淫羊藿含量甚微;用主成分分析法进行综合质量排序,淫羊藿、箭叶淫羊藿和柔毛淫羊藿排序靠前,同一品种随产地排序变化较大,黔岭淫羊藿及掺假的淫羊藿商品排序最后;聚类分析显示在植物来源、产地、炮制和含量方面有较好区分意义;以淫羊藿(E. brevicornu)建立共有模式,9种淫羊藿共标定了8个共有峰,相似度范围在0.066~0.979,不同品种间存在一定差异。结论: 指纹图谱相似度和含量测定的化学模式均能良好的评价淫羊藿品种和质量。
Abstract
Objective: To investigate the source and quality of commercially available Epimedium based on HPLC fingerprint and multi-index assay, so as to provide reference for its quality evaluation and resource development. Methods: Forty batches of commercially available epimedium were collected. Fingerprint analysis and determination of the total flavonoid glycosides were performed by HPLC method. The total flavonoid glycosides were determined by UV method. And the contents of their extracts were determined. Stoichiometric analysis was employed to perform the comprehensive quality evaluation of different medicinal materials of Epimedium. Results: There were 9 different plant sources of Epimedium in the market. The five authentic Epimedium were of good quality, and the local varieties were coarse Epimedium. The contents were high in Epimedium from Hunan, while the contents were low in Epimedium from Qianling and Sichuan. The comprehensive quality ranking of Epimedium indicated that Epimedium Fletchum and Epimedium Fletchum ranked first by the principal component analysis method. The ranking of the same variety varied greatly with different habitats, and the Qianling Epimedium and adulterated Epimedium ranked last. The results of cluster analysis and principal component analysis were basically consistent. The cluster analysis showed distinguishing significance in plant sources, habitats, processing methods and contents. Based on the common pattern of E. brevicornu, a total of 8 common peaks were identified for 9 species of E. Brevicornu, and their similarities ranged from 0.066 to 0.979. Differences were observed among different species. Conclusion: Both the fingerprint similarity and the chemical model of content determination can be used to evaluate the species and quality of Epimedium.
关键词
淫羊藿 /
指纹图谱 /
含量测定 /
主成分分析 /
聚类分析 /
相似度评价 /
质量评价
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Key words
Epimedium /
fingerprints /
content determination /
principal component analysis /
cluster analysis /
similarity evaluation /
quality evaluation
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