Objective: To develop an HPLC method for the simultaneous determination of 8 major components （ephedrine hydrochloride,pseudoephedrine hydrochloride,amygdalin,liquiritin,hesperidin,baicalin, baicalein,wogonin） in Baikejing granules. Methods: The column of Diamonsil C18 with the mobile phase of ethanol-0.1% phosphoric acid at a flow rate of 1.0 mL·min-1,the detection wavelength was 215 nm for ephedrine, pseudoephedrine and amygdalin,280 nm for liquiritin,hesperidin,baicalin,baicalein,wogonin,respectively． The temperature of column was 45 ℃,and the injection volume was 10 μL. Results: Determination results of ephedrine hydrochloride,pseudoephedrine hydrochloride,amygdalin,liquiritin,hesperidin,baicalin,baicalein, wogonin were 0.312-1.018 mg·g-1,0.321-1.175 mg·g-1,0.315-0.723 mg·g-1,0.876-1.735 mg·g-1,0.982- 2.604 mg·g-1,0.824-9.999 mg·g-1,1.635-2.888 mg·g-1,0.812-1.661 mg·g-1,respectively．Eight components showed good linear relationship within their own ranges（r ≥ 0.999）,whose average recoveries were 97.4%-99.7% with RSDs 0.65%-1.5%. Conclusion: The present method is accurate and simple,which can be used for the quality control of Baikejing granules．
[1] 中华人民共和国药典2015 年版.一部[S].2015:191,301,320,201,86
ChP2015.Vol Ⅰ[S].2015:191,301,320,201,86
[2] 张珂,许霞,李婷,等.利用UHPLC-IT-TOF-MS 分析陈皮的化学成分组[J].中国中药杂志,2020,45(4):899
ZHANG K,XU X,LI T,et al.Chemome profiling of Citri Reticulatae Pericarpium using UHPLC-IT-TOF-MS[J].China J Chin Mater Med,2020,45(4):899
[3] 吴惠君,欧金龙,池晓玲,等. 陈皮药理作用研究概述[J]. 实用中医内科杂志,2013,27(17):91
WU HJ,OU JL,CHI XL,et al.Overview of pharmacological effects of Chenpi[J].J Pract Tradit Chin Intern Med,2013,27(17):91
[4] 李尚科,李跑,杜国荣,等.陈皮组成成分及检测方法研究进展[J].中国果菜,2019,39(10):36
LI SK,LI P,DU GR,et al.Progress in composition and determination methods of Pericarpium Citri Reticulatae[J].China Fruit Veget,2019,39(10):36
[5] 李欣然,王静,余晓燕,等.陈皮的研究进展[J].中国医药指南,2016,14(24):31
LI XR,WANG J,YU XY,et al.Advances of research work in Citri Reticulatae Pericarpium[J].Guide China Med,2016,14(24):31
[6] 许姗姗,许浚,张笑敏,等.常用中药陈皮、枳实和枳壳的研究进展及质量标志物的预测分析[J]. 中草药,2018,49(1):35
XU SS,XU J,ZHANG XM,et al.Research progress on Citri Reticulatae Pericarpium,Aurantii Fructus Immaturus,and Aurantii Fructus and Q-marker predictive analysis[J].Chin Tradit Herb Drugs,2018,49(1):35
[7] DING Y,DOU J,TENG Z,et al.Antiviral activity of baicalin against influenza A（H1N1/H3N2) virus in cell culture and in mice and its inhibition of neuraminidase[J].Arch Virol,2014,159(12):3269
[8] 张晓娟,吕勃川.黄芩研究新进展[J].中医药学报,2017,45(1):96
ZHANG XJ,LÜ BC.New research progress in Radix Scutellariae[J].Acta Chin Med Pharmacol,2017,45(1):96
[9] 施高翔,邵菁,汪天明,等.黄芩及其有效成分抗菌作用新进展[J].中国中药杂志,2014,39(19):3713
SHI GX,SHAO J,WANG TM,et al.New advance in studies on antimicrobal activity of Scutellaria baicalensis and its effective ingredients[J].China J Chin Mater Med,2014,39(19):3713
[10] 杨昕宇,肖长芳,张凯熠,等.麻黄临床应用与药理作用研究进展[J].中华中医药学刊,2015,33(12):2874
YANG XY,XIAO CF,ZHANG KY,et al.Research progress on clinical application and pharmacological functions of Ephedra[J]. Chin Arch Tradit Chin Med,2015,33(12):2874
[11] 施松善,崔健,王顺春,等.麻黄化学成分与药理作用研究进展[J].中国中药杂志,2006,31(20):1661
SHI SS,CUI J,WANG SC,et al.Advances in research of chemical constituents and pharmacological activites of Ephedra[J].China J Chin Mater Med,2006,31(20):1661
[12] 张梦婷,张嘉丽,任阳阳,等.麻黄的研究进展[J].世界中医药,2016,11(9):1917
ZHANG MT,ZHANG JL,REN YY,et al.Research progress in Ephedrae Herba[J].World Chin Med,2016,11(9):1917
[13] 范贤哲,李广凯,蒋艳杰,等.苦杏仁中苦杏仁苷提取工艺研究[J].广州化工,2018,46(10):81
FAN XZ,LI GK,JIANG YJ,et al.Study on extraction technology of amygdalin in Armeniacae Semen Amarum[J].Guangzhou Chem Ind,2018,46(10):81
[14] 李冀,李想,曹明明,等.甘草药理作用及药对配伍比例研究进展[J].上海中医药杂志,2019,53(7):83
LI J,LI X,CAO MM,et al.Research progress in pharmacological actions of liquorice and proportion of couplet medicines in combination[J].Shanghai J Tradit Chin Med,2019,53(7):83
[15] 李洪亮,王珊珊,刘明,等.高效液相色谱法测定百咳静颗粒(儿童型)中黄芩苷的含量[J].广东药学院学报,2011,27(3):277
LI HL,WANG SS,LIU M,et al.Determination of baicalin in Baikejing granules(for children) by HPLC[J].J Guangdong Pharm Univ,2011,27(3):277
[16] 付春梅,李章万,刘三康,等.乙醇- 水作为反相高效液相色谱流动相的研究[J].色谱,1999,17(5):466
FU CM,LI ZW,LIU SK,et al.The study of ethanol-water used as reversed-phase high performance liquid chromatographic mobile phase[J].Chin J Chromatogr,1999,17(5):466
[17] 陈繁华,曾玉梅.对《中国药典》麻黄含量测定方法的改进[J].今日药学,2016,26(2):110
CHEN FH,ZENG YM.Improvement of determination method of Ephedra content in ChP[J].Pharm Today,2016,26(2):110
[18] 刘亚飞. 关于中国药典2005 年版截止波长问题的几点建议[J].中国药品标准,2007,8(5):325
LIU YF.Suggestions on cut-off wavelength item in ChP 2005 edition[J].Drug Stand China,2007,8(5):325
