目的：建立消渴灵片中山麦冬的专属性检测方法。方法： 样品经甲醇-氨水超声提取,利用高分离度快速液相色谱-三重串联四极杆质谱（RRLC-QQQ/MS）检测山麦冬。采用 Thermo Accla imTM RSLC 120 C18 色谱柱（2.1 mm×100 mm,1.8 μm）,以乙腈（A）-20 mmol·L-1乙酸铵溶液（B）为流动相,梯度洗脱（0~20 min,5%A → 90%A）,流速 0.2 mL·min-1,柱温 30 ℃,进样量 2 μL。离子化模式为 ESI+,选择 m/z 723.4 → 251.2 和 m/z 723.4 → 269.2 作为山麦冬皂苷 B 检测离子对,m/z 893.5 → 463.1 和 m/z 893.5 → 481.2 作为短葶山麦冬皂苷 C 检测离子对,进行多反应监测。结果：山麦冬皂苷 B 和短葶山麦冬皂苷 C 的检测下限分别为 0.28 μg·片 -1 和 0.20 μg·片 -1。111 批次消渴灵片中有 8 家企业的 25 批样品掺伪,其中 6 批次样品同时掺入湖北麦冬和短葶山麦冬,4 批次样品为湖北麦冬掺伪,15 批次样品为短葶山麦冬掺伪。结论：所建方法专属、准确、快速、简便,可为消渴灵片的专项治理提供有力的技术支持。
Objective：To establish a method for the specific detection of Liriopes Radix in Xiaokeling tablets. Methods：The samples were extracted by methanol-ammonia,rapid resolution liquid chromatography coupled with triple quadrupole electrospray tandem mass spectrometry（RRLC-QQQ/MS）was used to detect Liriopes Radix． The Thermo AcclaimTMRSLC 120 C18（2.1 mm×100 mm,1.8 μm）column was used．Acetonitrile -20 mmol·L-1ammonium acetate solution was used as mobile phase,and gradient elution（0~20 min,5%A → 90%A）,flow rate was 0.2 mL·min-1．Column temperature was 30 ℃,injection was 2 μL．The ionized mode was ESI+,and m/z 723.4 → 251.2 and m/z 723.4 → 269.2 were selected as the ion pairs of liriopeside B,and m/z 893.5 → 463.1 and m/z 893.5 → 481.2 as the ion pairs of Liriope muscari Baily saponin C,for multi-reaction monitoring. Results：The detection limits of liriopeside B and Liriope muscari Baily saponin C were 0.28 μg per tablet and 1.50 μg per tablet, respectively．6 batches of samples from batch 111 Xiaokeling tablets were mixed with Liriope spicata var．prolifera Y．T．Ma and Liriope muscari（Decne．）Bailey,4 batches of samples were adulterated with Liriope spicata var． prolifera Y．T．Ma,and 15 batches of samples were adulterated with Liriope muscari（Decne．）Bailey,involving 25 batches of samples from 8 enterprises. Conclusion：The established method is accurate,rapid and simple,which can provide strong technical support for the special treatment of Xiaokeling tablets．
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