成分分析

不同生长期穿心莲药材HPLC 指纹图谱及化学模式识别

展开
  • 山东大学第二医院药房, 济南 250000
第一作者: Tel: (0531)88197777;E-mail: 5948330233@qq.com

收稿日期: 2020-05-19

  网络出版日期: 2024-05-31

HPLC Fingerprint and chemical pattern recognition of Andrographis Herba with different growth period

Expand
  • The Second Hospital Pharmacy of Shandong University, Jinan 250000, China

Received date: 2020-05-19

  Online published: 2024-05-31

摘要

目的: 建立不同生长期穿心莲药材HPLC 指纹图谱, 并结合化学模式识别技术对其进行质量评价。方法: 采用Agilent Eclipse XDB-C18 色谱柱(250 mm×4.6 mm, 5 μm);乙腈-0.2% 磷酸水溶液为流动相, 梯度洗脱;流速1.0 mL·min-1;检测波长225、250 nm。采用中药指纹图谱相似度评价系统建立指纹图谱共有模式和相似度计算, 并采用SPSS22.0 和SIMCA-P14.1 软件建立聚类分析(cluster analysis, CA)、主成分分析(principal component analysis, PCA)和正交偏最小二乘法-判别分析(orthogonal partial least square discriminate analysis, OPLS-DA)模型做统计分析。结果: 建立了穿心莲药材指纹图谱, 共标定17 个共有峰, 经与对照品比对指认出其中4 个化合物。18 批供试品的相似度为0.902~0.997, PCA 分析出不同生长期穿心莲药材最佳采收期为盛花前期;OPLS-DA 将不同生长期穿心莲药材区分, 并筛选5 个差异标记物。结论: HPLC 指纹图谱能够反映穿心莲药材不同生长期的差异, 结合化学模式识别技术, 可为穿心莲药材的采收和质量评价提供参考。

本文引用格式

王震 . 不同生长期穿心莲药材HPLC 指纹图谱及化学模式识别[J]. 药物分析杂志, 2021 , 41(3) : 410 -420 . DOI: 10.16155/j.0254-1793.2021.03.05

Abstract

Objective: To establish an HPLC fingerprint of Andrographis Herba in different growth period, and to evaluate the quality through chemical pattern recognition. Methods: The analysis was performed on Agilent Eclipse XDB-C18(250 mm×4. 6 mm, 5 μm))column with mobile phase consisted of acetonitrile-0. 2% phosphoric acid solution(gradient elution)at a flow rate of 1. 0 mL·min-1. The detection wavelengths were set at 225 and 250 nm. The fingerprint common pattern and similarity calculation were established using traditional Chinese medicine chromatographic fingerprint similarity evaluation system. The cluster analysis(CA), principal component analysis (PCA), and orthogonal partial least square discriminate analysis(OPLS-DA) models were established using SPSS22. 0 and SIMCA-P14. 1 software to perform statistical analysis. Results: HPLC fingerprint of Andrographis Herba was established successfully and 17 common peaks were identified in the study, 4 chemical compounds were identified using standard references. The similarity calculation results were 0. 902~0. 997. PCA showed that the optimum harvesting stage of Andrographis Herba in different growth period was early flowering stage and PLSDA screened out 5 differential markers. Conclusion: The HPLC fingerprint can reflect the differences in different growth period, combining PCA and PLS-DA can provide reference for the harvesting and quality evaluation of Andrographis Herba.

参考文献

[1] 中华人民共和国药典2015 年版. 一部[S]. 2015: 268
ChP 2015. Vol ⅠhP 2015. Vol Ⅰ[S]. 2015: 268
[2] 王芳, 翟文婷, 李艳丽, 等. 穿心莲内酯制剂及药代动力学的研究进展[J]. 中国药业, 2013, 22(9): 1
WANG F, ZHAI WT, LI YL, et al. Research progress on new preparations and pharmacokinetic of andrographolide[J]. China pharm, 2013, 22(9): 1
[3] 张晓, 唐力英, 吴宏伟, 等. 穿心莲现代研究进展[J]. 中国实验方剂学杂志, 2018, 24(18): 222
ZHANG X, TANG LY, WU HW, et al. Development of modern research on Andrographis paniculatee[J]. Chin J experi Tradit Med Form, 2018, 24(18): 222
[4] 王刚, 孙丹丹, 耿子凯, 等. 穿心莲抗病毒作用研究进展[J]. 辽宁中医药大学学报, 2019, 21(7): 108
WANG G, SUN DD, GENG ZK, et al. Research progress on the anti-virus effect of Andrographis paniculate(Birm. f.) Ness[J]. J Liaoning Univ TCM, 2019, 21(7): 108
[5] 刘江碧, 金典, 陈思敏. 穿心莲内酯的药理学作用研究现状[J]. 中药与临床, 2019, 10(Z1): 57
LIU JB, JIN D, CHEN SM. Current pharmacological research of andrographolide[J]. Pharm Clin Chin Mater Med, 2019, 10(Z1): 57
[6] 高俊丽. 不同生长期穿心莲化学成分及相关酶活性变化规律的研究[D]. 广州: 广州中医药大学, 2016
GAO JL. Anlysis of Enzyme and Diterpene Lactone Variability During Ontogenesis of Andrographis paniculata(Burm. f.) Nees [D]. Guanghou: Guanghou University of Chinese Medicine, 2016
[7] 陈两绵, 高慧敏, 朱晶晶, 等. 不同采收及加工方式对穿心莲药材质量的影响[J]. 中国中药杂志, 2020, 45(8): 1717
CHEN LM, GAO HM, ZHU JJ, et al. Effects of different harvesting and processing methods on quality of Andrographis Herba[J]. China J Chin Mater Med, 2020, 45(8): 1717
[8] 莫建霞, 祝晨蔯, 张晓君, 等. 不同生长期穿心莲中穿心莲内酯和脱水穿心莲内酯含量的动态研究[J]. 中药新药与临床药理, 2003(2): 134
MO JX, ZHU CD, ZHANG XJ, et al. Determination of andrographolide and 14-deoxy-11, 12-didehydroandrographolide from Herba Andrographis in various growing periods[J]. Tradit Chin Drug Res Clin Pharmacol, 2003(2): 134
[9] 周思敏, 刘斌. 中药指纹图谱相似度软件在“药物分析”教学中的应用[J]. 林区教学, 2020(1): 23
ZHOU SM, LIU B. Application of fingerprint similarity software of traditional chinese medicine in“ pharmaceutical analysis” teaching[J]. Teach Forestry Region, 2020(1): 23
[10] 田甜, 韩蕊莲, 梁宗锁. 不同产地和不同年限黄芩HPLC 指纹图谱研究[J]. 浙江农业科学, 2018, 59(3): 370
TIAN T, HAN RL, LIANG ZS. Study on HPLC fingerprint of Scutellaria baicalensis Georgi from different habitats and ages[J]. J Zhejiang Agr Sci, 2018, 59(3): 370
[11] 杨贵雅, 薛紫鲸, 张丹, 等. 基于指纹图谱和多成分含量测定的丹参药材皮部和木部化学成分比较研究[J]. 中草药, 2019, 50(21): 5328
YANG GY, XUE ZJ, ZHANG D, et al. Chemical comparison of Salvia miltiorrhiza phloem and xylem based on fingerprint analysis and quantitative analysis of multi-components[J]. Chin Tradit Herb Drugs, 2019, 50(21): 5328
[12] 顾志荣, 师富贵, 金岩. 化学模式识别在中药分析中的应用研究[J]. 甘肃中医药大学学报, 2014, 31(3): 108
GU ZR, SHI FG, JIN Y. Application of chemical pattern recognition in analysis of traditional Chinese Medicine[J]. J Gansu Univ Chin Med, 2014, 31(3): 108
[13] 孙立丽, 王萌, 任晓亮. 化学模式识别方法在中药质量控制研究中的应用进展[J]. 中草药, 2017, 48(20): 4339
SUN LL, WANG M, REN XL. Application progress on chemical pattern recognition in quality control of Chinese materia medica[J]. Chin Tradit Herb Drugs, 2017, 48(20): 4339
[14] 汪剑飞, 吴静, 胡杰, 等. 双波长HPLC 法同时测定穿心莲浸膏中穿心莲内酯和脱水穿心莲内酯的含量[J]. 安徽医药, 2014, 18(7): 1237
WNAG JF, WU J, HU J, et al. Simultaneous determination of andrographolide and dehydroandrographolide in extractum andrographis by dual-wavelength HPLC[J]. Anhui Med Pharm J, 2014, 18(7): 1237
[15] 詹十音. HPLC 法同时测定复方穿心莲片中4 种成分的含量[J]. 中国药房, 2016, 27(24): 3425
SHAN SY. Simultaneous determination of four components in compound chuanxinlian tablet by HPLC[J]. China Pharm, 2016, 27(24): 3425
[16] 周汝顺, 江燕, 陈启友, 等. 穿心莲超声提取工艺研究[J]. 中国兽药杂志, 2019, 53(8): 66
ZHOU RS, JIANG Y, CHEN QY, et al. Study on ultrasonic extraction process of Andrographis paniculata[J]. Chin J Vetery Drug, 2019, 53(8): 66
[17] 霍保军, 杨琴, 刘鑫, 等. 正交试验优化穿心莲注射液制备工艺[J]. 亚太传统医药, 2016, 12(15): 45
HUO BJ, YANG Q, LIU X, et al. Optimization preparation process of Chuanxinlian injection by orthogonal design[J]. Asia-Pacific Tradit Med, 2016, 12(15): 45
[18] 廖延武, 吴德玲, 许凤清, 等. 基于聚类分析和主成分分析评价不同产地荆芥药材质量[J]. 安徽中医药大学学报, 2020, 39(1): 78
LIAO YW, WU DL, XU FJ, et al. Quality evaluation of fineleaf schizonepeta herb from different producing areas based on cluster analysis and principal component analysis[J]. J Anhui Univ Chin Med, 2020, 39(1): 78
[19] 刘明颖, 徐滢, 廖小菁, 等. 苦木茎HPLC 指纹图谱的建立及化学模式识别研究[J]. 中药材, 2019(11): 2603
LIU JY, XU Y, LIAO XQ, et al. Establishment of HPLC fingerprint and chemical pattern recognition of stem of Sophora flavescens[J]. J Chin Med Mater, 2019(11): 2603
[20] 张丽, 钱大玮, 卜凡淑, 等. 基于UPLC-MS 的黄芪药材质量评价研究[J]. 药物分析杂志, 2020, 40(4): 722
ZAHGN L, QIAN DW, BU FS, et al. Study on quality evaluation of Astragali Radix based on UPLC-MS[J]. Chin J Pharml Anal, 2020, 40(4): 722
[21] 阿基业, 何骏, 孙润彬. 代谢组学数据处理——主成分分析十个要点问题[J]. 药学学报, 2018, 53(6): 929
A JY, HE J, SUN RB. Multivariate statistical analysis for metabolomic data: the key points in principal component analysis[J]. Acta Pharm Sin, 2018, 53(6): 929
文章导航

/