Objective: To establish an HPLC characteristic chromatogram of pine needles of cedrus deodara and to develop simultaneous determination method of protocatechuic acid, protocatechuic aldehyde, dihydromyricetin, caffeic acid, ferulic acid and rutin in the sample. Methods: HPLC analysis was performed on a CAPCELL PAK-C18 column（250 mm×4. 6 mm, 5 μm）. The mobile phase consisted of methanol（A）and 0. 1% phosphoric acid aqueous solution（B） with gradient elution. The flow rate was 1 mL·min-1, the detection wavelength was 300 nm and the column temperature was 30 ℃. TCM chromatographic fingerprint similarity evaluation software（2012. 130723 version）was used for similarity analysis in 14 batches of pine needles of Cedrus deodara, and the contents of six components in 14 batches of samples were determined at the same time. Results: The similarities between the characteristic chromatogram and chromatograms of 14 batches of pine needles in Cedrus deodara were between 0. 932 and 0. 992. There were altogether 22 common peaks, and six of them were identified. The contents of protocatechuic acid, protocatechuic aldehyde, dihydromyricetin, caffeic acid, ferulic acid and rutin in 14 batches of samples were in the range of 34. 312-1 216. 750 μg·g-1, 5. 246-12. 533 μg·g-1, 43. 912-132. 047 μg·g-1, 50. 791-145. 021 μg·g-1, 13. 543-70. 379 μg·g-1 and 82. 763-243. 758 μg·g-1, respectively. Conclusion: The combination of the HPLC characteristic chromatogram and content determination of six components can reflect inherent quality of pine needles of Cedrus deodara. The proposed method is simple, feasible, rapid and accurate, providing reference for quality evaluation of the drug.
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