目的: 建立一测多评法同时测定活血丸（当归、红花、大黄、猪牙皂、牵牛子）中没食子酸、儿茶素、阿魏酸、大黄酸、大黄素、大黄酚6 个成分的含量。方法: 该药物甲醇提取液的分析采用Agilent ZORBAX SBC18色谱柱（250 mm×4. 6 mm, 5 μm）;流动相甲醇-0. 1% 磷酸水溶液, 梯度洗脱;体积流量1. 0 mL·min-1;柱温30 ℃ ;检测波长为225、245、278、320 nm。以大黄酸为内标, 计算其他5 个成分相对校正因子, 测定其含量。结果: 6 个成分在各自浓度范围内呈良好的线性关系（r ≥ 0. 999 7）, 平均加样回收率为97. 2%~100. 8%（RSD 为1. 6%~2. 0%）。一测多评法所得6 个成分含量数据与外标法接近。结论: 该方法简便、快捷, 且稳定性好, 准确度高, 可为活血丸新药研发的质量控制研究提供依据。
Objective: To establish a quantitative analysis of multi-components by single-marker（QAMS） method for the simultaneous content determination of gallic acid, catechin, ferulic acid, rhein, emodin and chrysophanol in Huoxue pills（angelica sinensis, safflower, rhubarb, fructus gleditsiae and morning glory）. Methods: The analysis of methanol extract of this drug was performed on a thermostatic Agilent ZORBAX SBC18 column（250 mm×4. 6 mm, 5 μm）at 30 ℃ with the mobile phase comprising of methanol-0. 1% phosphoric acid aqueous solution at 1. 0 mL·min-1 in a gradient elution manner, and the detection wavelengths were set at 225 nm, 245 nm, 278 nm and 320 nm. Rhein was used as an internal standard to calculate the relative correction factors of the other seven constituents, after which the content determination was made. Results: Six constituents showed good linear relationships within their own ranges（r ≥ 0. 9997）, whose average recoveries were 97. 2%-100. 8% with RSDs of 1. 6%-2. 0%. The results obtained by QAMS approximated those obtained by external standard method. Conclusion: The method is simple, rapid, stable and accurate, and it can provide the basis for the quality control of Huoxue pills.
[1] 中华人民共和国卫生部药典委员会. 中药部颁标准(中药成方制剂2 册)[S]. 1993: 182
Pharmacopoeia Committee of the Ministry of Health Of The People’s Republic of China. Standards Issued by the Ministry of Traditional Chinese Medicine(2 volumes of Traditional Chinese Medicine Prescriptions)[S]. 1993: 182
[2] 牛莉, 于泓苓. 中药当归的化学成分分析与药理作用研究[J]. 中西医结合心血管病电子杂志, 2018, 6(21): 90
NIU L, YU HL. Chemical constituents and pharmacological effects of Angelica sinensis[J]. Cardiovasc Dis J Integr Tradit Chin West Med, 2018, 6(21): 90
[3] 尹辉. 当归化学成分及药理活性研究进展[J]. 重庆科技学院学报(自然科学版), 2015, 17(1): 100, 112
YIN H. The Latest research progress on chemical composition and pharmacological action of Angelica[J]. J Chongqing Insti Sci Technol(Nat Sci Edit), 2015, 17(1): 100, 112
[4] 李曦, 张丽宏, 王晓晓, 等. 当归化学成分及药理作用研究进展[J]. 中药材, 2013, 36(6): 1023
LI X, ZHANG LH, WANG XX, et al. Advances in studies on chemical components and pharmacological effects of Angelica sinensis[J]. J Chin Med Mater, 2013, 36(6): 1023
[5] 梁娜, 孙少平, 罗跃娥, 等. 阿魏酸的研究进展[J]. 黑龙江中医药, 2009, 38(3): 39
LIANG N, SUN SP, LUO YE, et al. Advances in ferulic acid[J]. Heilongjiang Tradit Chin Med, 2009, 38(3): 39
[6] 孙汉青, 李锦萍, 刘力宽, 等. 大黄化学成分与药理作用研究进展[J]. 青海草业, 2018, 27(1): 47
SUN HQ, LI JP, LIU LK, et al. Progress in research of chemical constituents and pharmacological actions of rhubarb[J]. Qinghai Pratacult, 2018, 27(1): 47
[7] 梁淋渊, 甘麦邻, 罗燕. 大黄化学成分与药理活性研究进展[J]. 中兽医医药杂志, 2017, 36(1): 80
LIANG LY, GAN ML, LUO Y. Advances in research on chemical components and pharmacological activities of rhubarb[J]. Chin J Vet Med, 2017, 36(1): 80
[8] 黄娟, 张庆莲, 皮凤娟, 等. 大黄的药理作用研究进展[J]. 中国医院用药评价与分析, 2014, 14(3): 282
HUANG J, ZHANG QL, PI FJ, et al. Advances in pharmacological effects of rhubarb[J]. Eval Anal Drug Use Hosp China, 2014, 14(3): 282
[9] 郑雪花, 杨君, 杨跃辉. 没食子酸药理作用的研究进展[J]. 中国医院药学杂志, 2017, 37(1): 94
ZHENG XH, YANG J, YANG YH. Research progress on pharmacological effects of gallic acid[J]. Chin Hosp Pharm J, 2017, 37(1): 94
[10] 徐先祥. 儿茶素的药理作用研究综述[J]. 郑州轻工业学院学报(自然科学版), 2012, 27(4): 60
XU XX. Review of research on pharmacological effects of catechins[J]. J Zhengzhou Univ Light Ind(Nat Sci), 2012, 27(4): 60
[11] 刘媛媛, 孟秀杰, 李丽静. 一测多评法同时测定梅翁退热颗粒中8种成分[J]. 中成药, 2019, 41(8): 1782
LIU YY, MENG XJ, LI LJ. Simultaneous determination of eight constituents in Meiweng Tuire granules by QAMS[J]. Chin Tradit Pat Med, 2019, 41(8): 1782
[12] MA H, YOKOYAMA S, SAIKI I, et al. Chemosensitizing effect of saikosaponin B on B16 F10 melanoma cells[J]. Nutr Cancer, 2017, 69(3): 506
[13] 艾悦言, 赵学勇, 付林, 等. HPLC 法同时测定三味龙胆花片中马钱苷酸和异金雀花素的含量[J]. 中国药房, 2018, 29(20): 2810
AI YY, ZHAO XY, FU L, et al. Simultaneous determination of loganic acid and isoscoparin in Sanwei Longdanhua tablets by HPLC[J]. China Pharm, 2018, 29(20): 2810
[14] 骆航, 李华生. 基于一测多评法的长城感冒片质量控制[J]. 药物分析杂志, 2020, 40(3): 536
LUO H, LI HS. Quality control of Changcheng Ganmao tablets based on QAMS[J]. Chin J Anal Pharm, 2020, 40(3): 536
[15] 李小琪, 范燕豪, 陈阳, 等. 一测多评法同时测定广藿香中4 种成分[J]. 中成药, 2019, 41(8): 1884
LI XQ, FAN YH, CHEN Y, et al. Simultaneous determination of four constituents in Pogostemon cablin by QAMS[J]. Chin Tradit Pat Med, 2019, 41(8): 1884
