Objective: To establish a quantitative analysis of multi-components by single marker (QAMS) for simultaneous determination of 7 flavanoids (5-hydroxy-6, 7, 3’, 4’-tetramethoxyflavone, apigenin, hispidulin, kaempferol, jaceosidin, eupatilin and casticin) in Artemisiae Argyi Folium. Methods: The HPLC system consisted of the Agilent ZORBAX SB-C18 column (150 mm×4.6 mm, 5 μm) column with gradient elution of acetonitrile and 0.2% phosphoric acid as the mobile phase at a flow rate of 1.0 mL·min-1, a detection wavelength of 350 nm, and a column temperature of 30 ℃. Eupatilin was selected as the internal reference substance, the relative correction factors between eupatilin and the other 6 flavanoids were established, and the contents of these 7 constituents in samples were calculated to realize QAMS. At the same time, compared with the external standard method to verify the accuracy and feasibility of the QAMS method. Results: Within a certain linear range, the relative correction factors between eupatilin and 5-hydroxy-6, 7, 3’, 4’-tetramethoxyflavone, apigenin, hispidulin, kaempferol, jaceosidin as well as casticin were 0.958, 1.387, 1.000, 0.950, 0.957 and 1.297, respectively (RSDs of RCFs were less than 2.0%). The contents of 5-hydroxy-6, 7, 3’, 4’-tetramethoxyflavone, apigenin, hispidulin, kaempferol, jaceosidin, eupatilin, casticin in 20 batches of Artemisiae Argyi Folium were 0.031 4-0.623 5 mg·g-1, 0.000 9-0.092 6 mg·g-1, 0.020 6-0.170 7 mg·g-1, 0.011 0-0.184 7 mg·g-1, 0.011 7-0.864 0 mg·g-1, 0.253 2-2.555 0 mg·g-1 and 0.015 6-0.250 7 mg·g-1, respectively. Conclusion: Using eupatilin as the internal reference, QAMS method for 7 flavanoids is established. The method is accurate and reliable, and can be used for quality control and quantitative analysis of Artemisiae Argyi Folium.
[1] 王智民, 高慧敏, 付雪涛, 等. 一测多评法中药质量评价模式方法学研究[J]. 中国中药杂志, 2006, 31(23):1925
WANG ZM, GAO HM, FU XT, et al. Multi-components quantitation by one marker new methond for quality evaluation of Chinese herbal medicine[J]. China J Chin Mater Med, 2006, 31(23):1925
[2] 王智民, 钱忠直, 张启伟. 一测多评法建立的技术指南[J]. 中国中药杂志, 2011, 36(6):657
WANG ZM, QIAN ZZ, ZHANG QW. The guidelines for establishement of QAMS[J]. China J Chin Mater Med, 2011, 36(6):657
[3] 中华人民共和国药典2010年版. 一部[S]. 2010: 76, 101, 416, 1182, 1493
ChP 2010. Vol Ⅰ[S]. 2010: 76, 101, 416, 1182, 1493
[4] 杜清, 陈志, 吴江, 等. 一测多评法同时测定斑花黄堇药材中4种生物碱成分[J]. 中草药, 2021, 52(19):6038
DU Q, CHEN Z, WU J, et al. Simultaneous determination of four alkaloids in different original Corydalis conspersa by QAMS[J]. Chin Tradit Herb Drugs, 2021, 52(19):6038
[5] 张作华, 褚光松, 王海燕, 等. 一测多评法同时测定苍耳子中5个酚酸类成分的含量[J]. 药物分析杂志, 2021, 41(9):1612
ZHANG ZH, CHU GS, WANG HY, et al. Simultaneous determination of five phenolic acids in Xanthii Fructus by QAMS[J]. Chin J Pharm Anal, 2021, 41(9):1612
[6] 于晓, 戴衍朋, 周倩, 等. 一测多评法测定艾叶中6个有机酸类成分的含量[J]. 药物分析杂志, 2016, 36(12):2131
YU X, DAI YP, ZHOU Q, et al. Simultaneous determination of six organic acids in Artemisiae Argyi Folium by QAMS[J]. Chin J Pharm Anal, 2016, 36(12):2131
[7] 彭瑶, 曾嵘, 吴尚洁, 等. 一测多评法测定藏族药烈香杜鹃中5种黄酮类成分的含量[J]. 中国中药杂志, 2021, 46(9):2229
PENG Y, ZENG R, WU SJ, et al. Content determination of five flavonoids in Tibetan medicine Rhododendron anthopogonoides by quantitative analysis of multi-components by single marker (QAMS)[J]. China J Chin Mater Med, 2021, 46(9):2229
[8] 徐硕, 徐文峰, 邝咏梅, 等. 一测多评法测定罗布麻叶中6个黄酮类成分的含量[J]. 药物分析杂志, 2019, 39(7):1217
XU S, XU WF, KUANG YM, et al. Determination of six flavanoids in Apocyni Veneti Folium by quantitative analysis of multi-components by single marker[J]. Chin J Pharm Anal, 2019, 39(7):1217
[9] 中华人民共和国药典2020年版. 一部[S]. 2020: 91
ChP 2020. Vol Ⅰ[S]. 2020: 91
[10] SONG XW, WEN X, HE JW, et al. Phytochemical components and biological activities of Artemisia argyi[J]. J Funct Food, 2019, 52: 648
[11] 马盖凡, 姜雪莲, 侯明槠, 等. 艾叶多糖提取、分离纯化及生物活性研究进展[J]. 中国医院药学杂志, 2022, 42(11):1164
MA GF, JIANG XL, HOU MZ, et al. Research progress in the extraction, separation, purification and biological activities of polysaccharides from leaves of Artemisia argyi[J]. Chin J Hosp Pharm, 2022, 42(11):1164
[12] 何柳, 王云鹏, 谢卫红, 等. 艾叶水提物和酸提物的抗氧化及抗菌活性比较[J]. 现代食品科技, 2021, 37(10):205
HE L, WANG YP, XIE WH, et al. Comparetive of antioxidat and antibacterial activities of aqueous and acidic extracts from Artemisia argyi[J]. Mod Food Sci Technol, 2021, 37(10):205
[13] XIAO JQ, LIU WY, SUN HP, et al. Bioactivity-based analysis and chemical characterization of hypoglycemic and antioxidant components from Artemisia argyi[J]. Bioorg Chem, 2019, 92: 103268
[14] 杨洋, 梅全喜, 杨光义, 等. 艾叶在古今瘟疫防治中的研究与应用[J]. 时珍国医国药, 2020, 31(2):438
YANG Y, MEI QX, YANG GY, et al. Research and application of Artemisia argyi in prevention and treatment of ancient and modern plague[J]. Lishizhen Med Mater Med Res, 2020, 31(2):438
[15] 蔡平. 艾叶的药理作用及应用[J]. 时珍国医国药, 2001, 12(12):1137
CAI P. Pharmacological action and application of Artemisia argyi[J]. Lishizhen Med Mater Med Res, 2001, 12(12):1137
[16] 马旭, 卫若楠, 于同月, 等. 艾叶的临床应用及其量效关系[J]. 吉林中医药, 2021, 41(11):1502
MA X, WEI RN, YU TY, et al. A study of the clinical application and dose-effect relationship of argy wormwood leaf[J]. Jilin J Chin Med, 2021, 41(11):1502
[17] 闫婷, 周正繁, 罗苗, 等. 不同产地艾叶挥发油的成分及含量分析[J]. 中药材, 2022, 45(10):2425
YAN T, ZHOU ZF, LUO M, et al. Composition and content of essential oil in Artemisia argyi leaves from different producing areas[J]. J Chin Med Mater, 2022, 45(10):2425
[18] 张元, 康利平, 郭兰萍, 等. 艾叶的本草考证和应用研究进展[J]. 上海针灸杂志, 2017, 36(3):245
ZAHNG Y, KANG LP, GUO LP, et al. Herbalogical study of mugwort leaves and research advances in its application[J]. Shanghai J Acup Moxib, 2017, 36(3):245
[19] 周洁, 李晔, 刘洋, 等. 不同产地艾叶中主要化学成分的研究现状分析[J]. 中草药, 2022, 53(15):4882
ZHOU J, LI Y, LIU Y, et al. Research status analysis of main chemical constituents in Artemisia argyi leaves from different producing areas[J]. Chin Tradit Herb Drugs, 2022, 53(15):4882
[20] 兰晓燕, 朱龙波, 黄显章, 等. 艾叶中主要化学成分的鉴定及其含量测定研究[J]. 中草药, 2021, 52(24):7630
LAN XY, ZHU LB, HUANG XZ, et al. Study on identification and quantitation of main compounds in Artemisiae Argyi Folium[J]. Chin Tradit Herb Drug, 2021, 52(24):7630
[21] 胡倩, 刘大会, 曹艳. 艾叶黄酮类化合物的研究进展[J]. 食品安全质量检测学报, 2019, 10(12):3648
HU Q, LIU DH, CAO Y. Research progress on flavonoids from Artemisia argyi[J]. J Food Saf Qual, 2019, 10(12):3648
[22] 兰晓燕, 张元, 朱龙波, 等. 艾叶化学成分、药理作用及质量研究进展[J]. 中国中药杂志, 2020, 45(17):4017
LAN XY, ZHANG Y, ZHU LB, et al. Research progress on chemical constituents from Artemisiae Argyi Folium and their pharmacological activities and quality control[J]. China J Chin Mater Med, 2020, 45(17):4017
[23] 胡倩, 李静, 刘大会, 等. 艾叶总黄酮提取物体内外抗氧化活性研究[J]. 食品工业科技, 2021, 42(6):304
HU Q, LI J, LIU DH, et al. Antioxidant activity of total flavonoids extracts from Folium of Artemisiae Argyi in vitro and in vivo[J]. Sci Technol Food Ind, 2021, 42(6):304
[24] 王小俊, 陈林霖, 方颖. 异泽兰黄素的药理作用研究进展[J]. 时珍国医国药, 2019, 30(3):665
WANG XJ, CHEN LL, FANG Y. Advances in pharmacological effect of eupatilin[J]. Lishizhen Med Mater Med Res, 2019, 30(3):665
[25] 王媛媛, 陈思涵, 许美婧, 等. 艾叶总黄酮与总多糖的提取工艺及其抗氧化性研究进展[J]. 广州化工, 2020, 48(18):13
WANG YY, CHEN SH, XU MJ, et al. Advances in the extraction process and antioxidant properties of total flavonoids and total polysaccharides from Artemisia argyi[J]. Guangzhou Chem Ind, 2020, 48(18):13
