代谢分析

黑骨藤主要化学成分在正常和类风湿性关节炎大鼠血浆、尿液、粪便中的代谢产物分析*

展开
  • 1.贵州医科大学 贵州省药物制剂重点实验室/药用植物功效与利用国家重点实验室,贵阳 550004;
    2.贵州医科大学药学院,贵阳 550004;
    3.贵州医科大学 民族药与中药开发应用教育部工程研究中心,贵阳 550004
第一作者 Tel:(0851)86908468;E-mail:793049231@qq.com
**Tel:(0851)86908468;E-mail:HUANGY2020@126.com

收稿日期: 2024-03-02

  网络出版日期: 2024-06-20

基金资助

*国家自然科学基金项目(U1812403、81660691);贵州省科技计划项目(黔科合平台人才-GCC[2022]031-1、CXTD[2023]019);贵州省教育厅自然科学研究项目(黔教合KY字[2021]033)

Analysis of metabolites of the main chemical components of Periploca forrestii in normal and rheumatoid arthritis rats’ plasma, urine and feces*

Expand
  • 1. Guizhou Provincial Key Laboratory of Pharmaceutics, State Key Laboratory of Functions and Applications of Medicinal Plants, Guiyang 550004, China;
    2. Guizhou Medical University of Pharmacy, Guiyang 550004, China;
    3. Engineering Research Center for the Development and Application of Ethnic Medicine and TCM Ministry of Education, Guizhou Medical University, Guiyang 550004, China

Received date: 2024-03-02

  Online published: 2024-06-20

摘要

目的: 研究黑骨藤在正常及佐剂性关节炎(AA)大鼠体内的代谢产物,探究类风湿性关节炎(RA)对黑骨藤有效成分在大鼠体内代谢的影响。方法: 采用弗氏完全佐剂制备AA大鼠模型;运用UPLC-Q TOF MSE方法,采用ACQUITY UPLC BEH C18(100 mm×2.1 mm,1.7 μm)色谱柱,以0.01%甲酸水-0.01%甲酸乙腈为流动相梯度洗脱,流速0.25 mL·min-1,采用电喷雾离子源,在负离子模式下,对正常和AA模型大鼠灌服黑骨藤提取物后的血浆、尿液和粪便进行分析。结果: 在正常大鼠体内和AA模型大鼠体内分别检测到2、3个原型成分,32、35个代谢产物,代谢途径主要包括单咖啡酰基奎宁酸还原、甲基化、开环裂解、葡萄糖醛酸化,二咖啡酰基奎宁酸还原、甲基化、乙酰化、异构化、硫酸酯化、葡萄糖醛酸化等。结论: AA模型大鼠血浆和尿液中的代谢产物比正常大鼠更具多样性,RA疾病状态可能会影响黑骨藤有效成分在体内的代谢途径。

本文引用格式

张熊莉, 夏涛, 郑林, 迟明艳, 李月婷, 巩仔鹏, 金阳, 刘亭, 黄勇 . 黑骨藤主要化学成分在正常和类风湿性关节炎大鼠血浆、尿液、粪便中的代谢产物分析*[J]. 药物分析杂志, 2024 , 44(4) : 594 -602 . DOI: 10.16155/j.0254-1793.2024.04.06

Abstract

Objective: To investigate the metabolites of Periploca forrestii in plasma, urine, and feces of normal and adjuvant arthritis(AA) rats, and explore the effect of rheumatoid arthritis(RA) on the metabolism of active components of P. forrestii. Methods: AA rat model was made by means of Freund’s complete adjuvant. Plasma, urine and feces of normal and AA rats were analyzed by UPLC-Q-TOF-MSE method using ACQUITY UPLC BEH C18 (100 mm×2.1 mm, 1.7 μm) column with 0.01% formic acid water-0.01% formic acid acetonitrile as mobile phase gradient elution at a flow rate of 0.25 mL·min-1, and electrospray ion source under negative ion mode. Results: Two and three prototype components, 32 and 35 metabolites were detected in normal rats and AA model rats. The metabolic pathways mainly include monocaffeoylquinic acid reduction, methylation, ring-opening cleavage, glucuronidation, dicaffeoylquinic acid reduction, methylation, acetylation, isomerization, sulfation glucuronidation, etc. Conclusion: The metabolites in plasma and urine of AA model rats are more diverse than those in normal rats, and the disease state of RA may affect the metabolic pathway of effective components of P. forrestii in the body.

参考文献

[1] 贵州省药品监督管理局. 贵州省中药材、民族药材质量标准[M]. 贵阳: 贵州科技出版社, 2003: 381
Guizhou Provincial Drug Administration. Quality Standards for Traditional Chinese Medicine and Ethnic Medicinal Materials in Guizhou Province[M] Guiyang: Guizhou Science and Technology Publishing House, 2003: 381
[2] 黄明进, 罗春丽, 郭刚, 等. 黑骨藤抗类风湿性关节炎作用及其分子机制[J]. 中国实验方剂学杂志, 2011, 17(12):174
HUANG MJ, LUO CL, GUO G, et al. Effects and molecular mechanism of Periploca forrestii against rheumatoid arthritis[J]. Chin J Exp Tradit Med Form, 2011, 17(12):174
[3] 包喜文, 李振英, 吴霞, 等. 黑骨藤追风活络胶囊对胶原诱导性关节炎大鼠IL-1β、IFN-γ、IL-15的影响[J]. 中国中医急症, 2018, 27(2):293
BAO XW, LI ZY, WU X, et al. Effects of Heiguteng Zhuifeng Huoluo capsules on serum IL-1β, IFN-γ and IL-15 of rats with collagen-induced-arthritis[J]. J Emerg Tradit Chin Med 2018, 27(2):293
[4] 杨波, 张建锋. 苗药黑骨藤研究进展[J]. 中国现代中药, 2019, 21(8):1122
YANG B, ZHANG JF. Research progress of Miao medicine Periploca forrestii[J]. Mod Chin Med, 2019, 21(8):1122
[5] 覃小丽, 陈浩, 夏涛, 等. 基于UPLC-Q-TOF-MS技术的黑骨藤血清药物化学分析[J]. 中国实验方剂学杂志, 2019, 25(6):125
QIN XL, CHEN H, XIA T, et al. Serum pharmacochemical analysis of Periploca forrestii rhizomes based on UPLC-Q-TOF-MS[J]. Chin J Exp Tradit Med Form, 2019, 25(6):125
[6] 张晓芳, 周芳, 王广基, 等. 疾病状态下的药物代谢处置变化及其机制研究[J]. 药物评价研究, 2019, 42(3):369
ZHANG XF, ZHOU F, WANG GJ, et al. Changes and related mechanisms of drug metabolism and disposition under diseases status[J]. Drug Eval Res, 2019, 42(3):369
[7] 潘洁, 王昌权, 李奎, 等. 基于大鼠类风湿性关节炎模型建立黑骨藤的PK-PD模型[J]. 中草药, 2020, 51(20):5194
PAN J, WANG CQ, LI K, et al. Pharmacokinetic-pharmacodynamic link model of Periploca forrestii based on rheumatiod arthritis rat model[J]. Chin Tradit Herb Drugs, 2020, 51(20):5194
[8] 夏涛, 王昌权, 李奎, 等. 黑骨藤提取物中3种活性成分在AA模型大鼠体内的药代动力学研究[J]. 中国药理学通报, 2020, 36(9):1270
XIA T, WANG CQ, LI K, et al. Pharmacokinetics of three active ingredients in Periploca forrestii extract after administrations in AA rats[J]. Chin Pharmacol Bull, 2020, 36(9):1270
[9] 王丽萍, 郭栋, 王果, 等. 中药绿原酸的研究进展[J]. 时珍国医国药, 2011, 22(4):961
WANG LP, GUO D, WANG G, et al. Advancement of chlorogenic acid in traditional Chinese medicine[J]. Lishizhen Med Mater Med Res, 2011, 22(4):961
[10] 覃小丽, 陈浩, 李奎, 等. UPLC-Q-TOF-MS法分析苗药黑骨藤提取物中的化学成分[J]. 中国药房, 2018, 29(21):2949
QIN XL, CHEN H, LI K, et al. Analysis of chemical compositions in Miao medicine Periploca forrestii extract by UPLC-Q-TOF-MS[J]. China Pharm, 2018, 29(21):2949
[11] WITTEMER SM, PLOCH M, WINDECK T, et al. Bioavailability and pharmacokinetics of caffeoylquinic acids and flavonoids after oral administration of Artichoke leaf extracts in humans[J]. Phytomedicine, 2005, 12(1-2):28
[12] DEL RIO D, STALMACH A, CALANI L, et al. Bioavailability of coffee chlorogenic acids and green tea flavan-3-ols[J]. Nutrients, 2010, 2(8):820
[13] TOMAS-BARBERAN F, GARCíA-VILLALBA R, QUARTIERI A, et al. In vitro transformation of chlorogenic acid by human gut microbiota[J]. Mol Nutr Food Res, 2014, 58(5):1122
[14] 巩仔鹏, 侯靖宇, 李梅, 等. 大鼠肠道菌群对羊耳菊提取物的代谢作用研究[J]. 中国中药杂志, 2017, 42(18):3584
GONG ZP, HOU JY, LI M, et al. Analysis of metabolites of Inula cappa extract in rat feces[J]. China J Chin Mater Med, 2017, 42(18):3584
[15] 李云, 周明眉, 邢丽娜, 等. 绿原酸的肠道菌群代谢研究进展[J]. 中草药, 2015, 46(4):610
LI Y, ZHOU MM, XING LN, et al. Advances in study on gut flora metabolism of chlorogenic acid[J]. Chin Tradit Herb Drugs, 2015, 46(4):610
[16] 谢芩, 钟大放, 陈笑燕. 鉴定大鼠注射绿原酸后体内的代谢产物[J]. 药学学报, 2011, 46(1):88
XIE Q, ZHONG DF, CHEN XY. Metabolites of injected chlorogenic acid in rats[J]. Acta Pharm Sin, 2011, 46(1):88
[17] 刘子菡, 尚展鹏, 王喻淇, 等. UHPLC-HRMS代谢组学研究硫熏麦冬对大鼠内源性代谢产物的影响[J]. 中国中药杂志, 2019, 44(21):4713
LIU ZH, SHANG ZP, WANG YQ, et al. UHPLC-HRMS metabonomics to study effect of sulfur-fumigated Ophiopogonis Radix on endogenous metabolites in rats[J]. China J Chin Mater Med, 2019, 44(21):4713
[18] 孙丽丽, 白海英, 郑文惠, 等. 基于UHPLC-Q-TOF-MS的当归补血汤治疗2型糖尿病小鼠的代谢组学研究[J]. 中国中药杂志, 2020, 45(3):636
SUN LL, BAI HY, ZHNEG WH, et al. Metabolomics study of Danggui Buxue Tang on treatment of type 2 diabetes mice using UHPLC-Q-TOF-MS[J]. China J Chin Mater Med, 2020, 45(3):636
[19] 王妮, 张娜, 李铁, 等. 人参干预脾气虚体质的非靶向代谢组学研究[J]. 中国中药杂志, 2020, 45(2):398
WANG N, ZHANG N, LI T, et al. Untargeted metabonomics study of ginseng in treatment of spleen-Qi deficiency[J]. China J Chin Mater Med, 2020, 45(2):398
文章导航

/