目的: 建立定量核磁共振氢谱(qHNMR)测定右佐匹克隆含量的方法。方法: 以1,3,5-三甲氧基苯δ 6.08处质子信号为内标峰,以右佐匹克隆 δ 8.54、8.38、8.12、7.78处质子信号为定量峰,以DMSO-d6为溶剂,在恒温298 K,弛豫延迟时间10 s,扫描次数32次条件下采集氢谱。结果: 定量核磁共振氢谱法测定右佐匹克隆片中右佐匹克隆平均含量为2.94%,即平均每片中含右佐匹克隆3.00 mg,RSD为0.15%,与药物说明标示的量基本一致。结论: 定量核磁共振氢谱可用于药物中右佐匹克隆含量的测定,并且具有快速、准确以及操作简单等优势。
Objective: To establish a quantitative 1H-NMR (qHNMR) method for the determination of dexzopiclone tablets content. Methods: Proton signal at δ 6.08 of 1,3,5-trimethoxybenzene and δ 8.54,8.38,8.12,7.78 of dexzopiclone were served as the internal standard and quantitative peaks,respectively. DMSO-d6 was employed as the solvent. The qHNMR spectra were acquired at 298 K with 10 s relaxation delay and 32 scanning times. Results: The content of dexzopiclone was estimated to be 2.94% (RSD=0.15%)by qHNMR method,which indicated that 3.00 mg of dexzopiclone was in one tablet. The result was consistent with the description of the label. Conclusion: qHNMR method can be used to determine the content of dexzopiclone. The method is accurate,simple and efficient.
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