目的:建立同时测定连钱草中3个黄酮类成分(木犀草素-7-O-β-D-葡萄糖醛酸(1→2)-O-β-D-葡萄糖醛酸苷、芹菜素-7-O-β-D-葡萄糖醛酸(1→2)-O-β-D-葡萄糖醛酸苷、木犀草素)及4个酚酸类成分(绿原酸、隐绿原酸、咖啡酸、迷迭香酸)含量的高效液相色谱法。方法:采用Waters SunFire C18(250 mm×4.6 mm,5 μm)色谱柱,以乙腈(A)-0.1%磷酸水溶液(B)为流动相,梯度洗脱,流速1.0 mL·min-1,检测波长330 nm,柱温30 ℃。结果:7个成分在各自范围内线性关系良好(r≥0.999 6);精密度(RSD≤0.87%)、稳定性(RSD≤1.1%)良好;平均加样回收率(n=6)为99.1%~101.1%,RSD为0.59%~1.7%。18批样品中绿原酸、隐绿原酸、咖啡酸、木犀草素-7-O-β-D-葡萄糖醛酸(1→2)-O-β-D-葡萄糖醛酸苷、芹菜素-7-O-β-D-葡萄糖醛酸(1→2)-O-β-D-葡萄糖醛酸苷、迷迭香酸和木犀草素含量范围分别为0.046~0.602、0.071~0.817、0.102~1.159、0.728~4.817、0.592~4.081、0.850~13.060和0.019~0.198 mg·g-1。结论:该方法简便易行,重复性好,可用于连钱草药材的质量控制。
Objective: To develop an HPLC method for simultaneous determination of three flavonoids (luteolin 7-O-β-glucuronyl-(1→2)-O-β-glucuronide, apigenin 7-O-β-glucuronyl-(1→2)-O-β-glucuronide and luteolin) and four phenolic acids (chlorogenic acid, cryptochlorogenic acid, caffeic acid and rosmarinic acid) in Glechomae Herba. Methods: The Waters SunFire C18 column(250 mm×4.6 mm,5 μm) was used, the mobile phase was acetonitrile(A)-0.1% phosphoric acid(B) with gradient elution, at the flow rate of 1.0 mL·min-1, the detection wavelength was 330 nm, and the column temperature was 30 ℃. Results: Seven constituents showed good linear relationships within their own ranges (r≥0.999 6), and the precision (RSD≤0.87%) and stability(RSD≤1.1%) were good. The average recoveries (n=6) were 99.1%-101.1% with the RSDs of 0.59%-1.7%. The contents of chlorogenic acid, cryptochlorogenic acid, caffeic acid, luteolin 7-O-β-glucuronyl-(1→2)-O-β-glucuronide, apigenin 7-O-β-glucuronyl-(1→2)-O-β-glucuronide, rosmarinic acid and luteolin in eighteen batches of samples were 0.046-0.602 mg·g-1, 0.071-0.817 mg·g-1, 0.102-1.159 mg·g-1, 0.728-4.817 mg·g-1, 0.592-4.081 mg·g-1, 0.850-13.060 mg·g-1 and 0.019-0.198 mg·g-1, respectively. Conclusion: This method is specific, accurate and reproducible, and can be used to control the quality of the Glechomae Herba.
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