目的:采用高效液相色谱建立管花肉苁蓉配方颗粒的指纹图谱及多成分含量测定方法,为管花肉苁蓉配方颗粒的鉴定及质量评价提供参考。方法:采用Agilent Eclipse Plus C18 (250 mm×4.6 mm,5 μm)色谱柱,以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,流速1.0 mL·min-1,柱温30 ℃,检测波长238 nm,进样量10 μL。结果:建立了管花肉苁蓉配方颗粒的指纹图谱,标定了12个共有峰,指认了京尼平苷酸、咖啡酸、松果菊苷、毛蕊花糖苷、管花苷A、异毛蕊花糖苷6个特征峰;并进一步建立了其含量测定方法,7批样品中上述6个成分含量分别为0.57~2.22、0.19~0.41、29.36~52.99、1.64~4.99、2.09~3.97、2.29~4.76 mg·g-1。结论:本研究所建立的管花肉苁蓉配方颗粒指纹图谱和6个成分含量测定的方法可行,可为全面控制和评价管花肉苁蓉配方颗粒的质量提供科学依据。
Objective: To provide reference for the identification and quality evaluation of Guanhuaroucongrong formula particles, and to establish the method of fingerprint and the multi-component content determination by HPLC. Methods: The separation was performed on an Agilent Zorbax C18 column (250 mm×4.6 mm, 5 μm) with acetonitrile-0.1% phosphoric acid aqueous solution as the mobile phase. The flow rate was 1.0 mL·min-1, the detection wavelength was 238 nm, the column temperature was 30 ℃, and the injection volume was 10 μL. Results: The fingerprint was established with 12 common peaks. 6 characteristic peaks including geniposidic acid, caffeic acid, echinoside, acteoside, tubuloside A and iso-acteoside were identified. The content determination method of them was further established. The contents of the above 6 components in 7 batches of samples were respectively 0.57-2.22 mg·g-1, 0.19-0.41 mg·g-1, 29.36-52.99 mg·g-1, 1.64-4.99 mg·g-1, 2.09-3.97 mg·g-1 and 2.29-4.76 mg·g-1. Conclusion: The method of fingerprint and content determination of 6 components established in this study is feasible, which can provide scientific basis for comprehensive control and evaluation of the quality of Guanhuaroucongrong formula particles.
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