目的:研究建立淫羊藿(箭叶淫羊藿)与其同属混淆品的生药学鉴别方法。方法:通过深入产区调研,收集准确基原的植物标本和样品,整理基原鉴定特征及商品现状,综合应用体式荧光显微镜、光学显微镜和扫描电镜技术等技术对淫羊藿(箭叶淫羊藿)与其混淆品进行了鉴别比较。结果:箭叶淫羊藿来源药材与其混淆品在叶片下表面非腺毛及突起等特征上存在明显差异。与箭叶淫羊藿显微特征接近的粗毛淫羊藿,在体式荧光显微镜、偏光显微镜和扫描电镜下也可实现有效区分。结论:本文研究归纳了淫羊藿(箭叶淫羊藿)与其同属混淆品较为专属且实用的鉴别特征,从而为临床用药、市场监督、日常检验、标准起草等工作提供有益的参考。
Objective: To study and establish a pharmacognostical identification method for Epmedii Folium (Epimedium sagittatum (Sieb. et Zucc) Maxim) and its adulterants from the same genus. Methods: On the basis of investigation, the accurate specimens and samples of Epmedii Folium (Epimedium sagittatum (Sieb.et Zucc) Maxim) and its adulterants were collected from the production areas, the identification characteristics and commodity status of the origin were sorted out, and the species were compared by using the techniques of stereo-fluorescence microscope, optical microscope and scanning electron microscope. Results: There were significant differences between Epmedii Folium (Epimedium sagittatum (Sieb.et Zucc) Maxim) and its adulterants in the characteristics of non-glandular hairs and protuberances on the lower surface of the leaves. Epimedium acuminatum Franch., which was similar to Epimedium sagittatum (Sieb.et Zucc) Maxim. in microscopic characteristics, could also be effectively distinguished under stereo-fluorescence microscope, polarizing microscope and scanning electron microscope. Conclusion: This study summarizes the specific and practical identification characteristics of Epmedii Folium (Epimedium sagittatum (Sieb.et Zucc) Maxim) and its adulterants, so as to provide useful references for clinical medication, market supervision, inspection, research of the standard and other related work.
[1] 中华人民共和国药典2020年版. 一部[S]. 2020:340
ChP 2020.Vol Ⅰ [S]. 2020:340
[2] 张华峰, 杨晓华, 郭玉蓉,等. 药用植物淫羊藿资源可持续利用现状与展望[J]. 植物学报, 2009, 44(3):363
ZHANG HF, YANG XH, GUO YR, et al. Current situation and prospects of sustainable utilization of medicinal plant Epimedium resources[J]. Chin Bull Botany, 2009, 44(3): 363
[3] 王可可, 龚其海. 淫羊藿化学成分及药理作用的研究进展[J]. 中国民族民间医药, 2015, 24(19):16
WANG KK, GONG QH. Research progress on chemical constituents and pharmacological effects of Epimedium [J]. Chin J Ethnomed Ethnopharm, 2015, 24(19):16
[4] 郭宝林, 黄文华, 孙娥,等. 淫羊藿药材和饮片市场调查[J]. 中国中药杂志, 2010, 35(13): 1687
GUO BL, HUANG WH, SUN E, et al. Market survey of Epimedium herbs and decoction pieces[J]. China J Chin Mater Med, 2010, 35(13): 1687
[5] 康帅,鲁静,张继,等. 淫羊藿药典品的基原调查及性状显微鉴别研究[J]. 中国中药杂志,2018, 43(4): 696
KANG S, LU J, ZHANG J, et al. Study on original investigation, macroscopic and microscopic identification of pharmacopoeia contained species of Epmedii Folium [J]. China J Chin Mater Med, 2018, 43(4): 696
[6] 中国科学院中国植物志编委会. 中国植物志.第29卷[M]. 北京: 科学出版社, 2001: 262
Editorial Committee of Flora of China, Chinese Academy of Sciences. Flora of China. Vol 29[M]. Beijing: Science Press, 2001: 262
[7] 何顺志. 中国淫羊藿属植物彩色图鉴[M]. 贵阳: 贵州科技出版社, 2014: 1
HE SZ. Chinese Epimedium Color Illustration[M]. Guiyang: Guizhou Science and Technology Publishing House Co.,Ltd., 2014: 1
[8] 徐国钧,徐珞珊.常用中药材品种整理和质量研究. 南方协作组. 第一册[M]. 福州:福建科学技术出版社,1994:745
XU GJ,XU LS. Sorting and Quality Research of Commonly Used Chinese Medicinal Materials. Southern Collaborative Group. Vol 1 [M]. Fuzhou:Fujian Science and Technology Press,1994:745
[9] 肖培根.新编中药志. 第三卷[M]. 北京:化学工业出版社,2002:322
XIAO PG. New compilation of Annals of Traditional Chinese Medicine. Vol Ⅲ[M]. Beijing: Chemical Industry Press, 2002: 322
[10] 尚志钧辑校. 神农本草经辑校[M]. 北京:学苑出版社, 2014: 107
Edited by SHANG ZJ. Compilation of Shennong Bencaojing [M]. Beijing: Academy Press, 2014: 107
[11] 尚志钧辑校. 名医别录(辑校本) [M]. 北京:人民卫生出版社, 1986: 143
Edited by SHANG ZJ. Mingyi Bielu(Compilation) [M]. Beijing: People’s Medical Publishing House, 1986: 143
[12] 尚志钧辑校. 本草经集注(辑校本) [M]. 北京:人民卫生出版社, 1994: 302
Edited by SHANG ZJ. Bencaojing Jizhu(Compilation) [M]. Beijing: People’s Medical Publishing House,1994: 302
[13] 苏颂.本草图经.尚志钧辑校 [M]. 合肥:安徽科学技术出版社,1994: 189
SU S. Compilation of Bencao Tujing. Edited by SHANG ZJ [M]. Hefei: Anhui Science and Technology Press,1994: 189
[14] 朱橚. 王锦绣,汤彦承译注. 救荒本草译注[M]. 上海:上海古籍出版社, 2015: 63
ZHU C. Annotated by WANG JX and TANG YC. Annotation of Jiuhuang Bencao [M]. Shanghai: Shanghai Classics Publishing House, 2015: 63
[15] 吴其濬. 植物名实图考[M]. 郑州:河南科学技术出版社, 2015:198
WU QJ. Zhiwuming Shitukao [M]. Zhengzhou: Henan Science and Technology Publishing House Co.,Ltd., 2015: 198
[16] 唐慎微. 证类本草 [M]. 北京:华夏出版社, 1993: 230
TANG SW. Zhenglei Bencao [M]. Beijing: Huaxia Publishing House, 1993: 230
[17] 陈嘉谟. 本草蒙筌(校点本) [M]. 合肥:安徽科学技术出版社,1987: 168
CHEN JM. Bencao Mengquan(Compilation) [M]. Hefei: Anhui Science and Technology Press,1987: 168
[18] 李时珍. 本草纲目[M]. 北京:人民卫生出版社, 1996: 750
LI SZ. Bencao Gangmu [M]. Beijing: People’s Medical Publishing House, 1996: 750
[19] 裴鑑,周太炎.中国药用植物志.第六册[M]. 北京:中国科学出版社,1958: 260
PEI J,ZHOU TY. Chinese Medicinal Flora. Vol Ⅵ [M]. Beijing: China Science Press, 1958: 260
[20] 高敏,冯学锋,郭宝林,等. 淫羊藿药典品种显微鉴别研究[J]. 中国中药杂志,2009,34(23):3123
GAO M,FENG XF,GUO BL, et al. Study on microscopic identification of Epimedium species in pharmacopoeia[J]. China J Chin Mater Med, 2009, 34(23): 3123
