目的:建立同时测定千里光中绿原酸、隐绿原酸、咖啡酸、芦丁、金丝桃苷、异槲皮苷、异绿原酸A、槲皮苷、异绿原酸C和槲皮素10个成分含量的HPLC方法。方法:采用YMC C18色谱柱(250 mm×4.6 mm,5 μm),以乙腈(A)-0.1%磷酸水溶液(B)为流动相,梯度洗脱,流速1.0 mL·min-1,检测波长350 nm,柱温30 ℃。结果:10个成分分别在各自相应浓度范围内与峰面积呈良好的线性关系(r≥0.999 8);精密度(RSD≤0.8%)、重复性(RSD≤1.7%)、稳定性(RSD≤1.6%)良好;平均回收率(n=6)为96.0%~100.6%,RSD为0.54%~1.8%;5批样品中绿原酸、隐绿原酸、咖啡酸、芦丁、金丝桃苷、异槲皮苷、异绿原酸A、槲皮苷、异绿原酸C和槲皮素含量范围分别为1.097~3.390、0.323~0.758、0.140~0.249、0.176~0.489、0.381~0.797、0.107~0.199、0.280~0.598、0.077~0.659、0.090~0.152和0.028~0.058 mg·g-1。结论:该方法简便易行,重复性好,可为千里光药材的质量控制指标提供一定的参考。
Objective: To establish an HPLC method for the simultaneous determination of ten chemical compositions including chlorogenic acid, cryptochlorogenic acid, caffeic acid, rutin, hyperoside, isoquercitrin, isochlorogenic acid A, quercitrin, isochlorogenic acid C, and quercetin in the aerial parts of Senecio scandens. Methods: The YMC C18 column(250 mm×4.6 mm, 5 μm) was used, the mobile phase was acetonitrile (A) -0.2% phosphate aqueous(B) with gradient elution, at the flow rate of 1.0 mL·min-1, the detection wavelength was 350 nm, and the column temperature was at 30 ℃. Results: The linear relationship of the ten chemical components was good(r ≥0.999 8). Results of precision(RSD≤0.8%), repeatability(RSD≤1.7%) and stability (RSD≤1.6%) were good. The average recoveries (n=6) were 96.0%-100.6% with RSDs of 0.54%-1.8%. The contents of chlorogenic acid, cryptochlorogenic acid, caffeic acid, rutin, hyperoside, isoquercitrin, isochlorogenic acid A, quercitrin, isochlorogenic acid C, and quercetin in five batches of samples were 1.097-3.390、0.323-0.758、0.140-0.249、0.176-0.489、0.381-0.797、0.107-0.199、0.280-0.598、0.077-0.659、0.090-0.152 and 0.028-0.058 mg·g-1, respectively. Conclusion: This method is specific, accurate and reproducible, and it can provide reference for the quality control of Senecio scandens.
[1] 中华人民共和国药典2020年版. 一部[S]. 2020: 35
ChP 2020. Vol Ⅰ[S]. 2020: 35
[2] 李燕, 赵鸿宾, 蒙慧彤. 中药千里光化学成分及药理作用的研究进展[J].广东化工, 2020, 47(10): 75
LI Y, ZHAO HB, MENG HT. Research progress on chemical constituents and pharmacological effects of the Senecio scandens Buch.-Ham.[J].Guangdong Chem Ind, 2020, 47(10): 75
[3] 张文平, 张文书, 王小丽, 等. 千里光抗金黄色葡萄球菌作用机制的血清药理学研究[J].时珍国医国药, 2009, 20(7): 1629
ZHANG WP, ZHANG WS, WANG XL, et al. Study on mechanism of Senecio scandens Buch.-Ham. against Staphylococcus aureus with serum pharmacology[J].Lishzhen Med Mater Med Res, 2009, 20(7): 1629
[4] 邹昊宇, 胡鸿运, 刘嫡, 等. 千里光通过NLRP3/Caspase-1/IL-1β通路对过敏性结膜炎大鼠角结膜炎症的影响[J].中药新药与临床药理, 2019, 30(11): 1346
ZOU HY, HU HY, LIU D, et al. Effect of Senecio scandens Buch.-Ham. on inflammation of cornea and conjunctiva in rats with allergic conjunctivitis via NLRP3/caspase-1/IL-1β pathway[J].Tradit Chin Drug Res Pharmacol, 2019, 30(11): 1346
[5] 周艳娟, 李翠芹, 王喆之. 羽叶千里光不同部位提取物的抗氧化活性研究[J].现代生物医学进展, 2008, 8(3): 513
ZHOU YJ, L CQ, WANG ZZ. Study on antioxidant activities of extracts from different parts of Senecio argunensis[J].Prog Mod Biomed, 2008, 8(3): 513
[6] 张静, 叶彬, 武卫华, 等. 千里光提取物体外抗阴道毛滴虫的效果观察[J].热带医学杂志, 2011, 11(2): 173
ZHANG J, YE B, WU WH, et al. The effects of the extracts of groundsel on the Trichomonas vaginalis in vitro[J].J Trop Med, 2011, 11(2):173
[7] 梅莹, 黄帅, 陈才, 等. 双花千里光中黄酮类成分的研究[J].华西药学杂志, 2012, 27(5): 496
MEI Y, HUANG S, CHEN C, et al. Study on the flavonoids of Senecio dianthus[J].West China J Pharm Sci, 2012, 27(5): 496
[8] 何忠梅, 宗颖, 孙佳明, 等. 千里光中几种黄酮和酚酸类成分的分离与鉴定[J].应用化学, 2010, 27(12): 1486
HE ZM, ZONG Y, SUN JM, et al. Isolation and identification of several flavonoids and phenolic acids from Senecio scandens Buch.-Ham.[J].Chin J Appl Chem, 2010, 27(12): 1486
[9] 谭道鹏, 陈莹, 季莉莉, 等. 千里光中的生物碱类成分[J].中国中药杂志, 2010, 35(19): 2572
TANG DP, CHEN Y, JI LL, et al. Alkaloids from Senecio scandens[J].China J Chin Mater Med, 2010, 35(19): 2572
[10] 周威, 何颖, 张连学. 全叶千里光挥发油成分GC-MS分析[J].中国当代医药, 2009,16(22): 141
ZHOU W, HE Y, ZHANG LX. GC-MS analysis of volatile constituents of Senecio cannabifolius Less. var. integrifolius (Kǒidz.) Kitam.[J].China Mod Med, 2009, 16(22): 141
[11] 律广富, 仇志凯, 常诗卓, 等. 玉米须总黄酮抗炎镇痛及对急性痛风性关节炎的改善作用研究[J].药物评价研究, 2018, 41(2): 206
LÜ GF, QIU ZK, CHANG SZ, et al. Anti-inflammatory, analgesic and analgesic effects of total flavone from Stigma maydis and its effect on acute gouty arthritis[J].Drug Eval Res, 2018, 41(2): 206
[12] 孙森凤, 张颖颖. 瓦松总黄酮的提取及其抑菌效果[J].中国食物与营养, 2017, 23(7): 34
SUN SF, ZHANG YY. Extraction and antibacterial effect of total flavonoids in Orostachys sp.[J].Food Nutr China, 2017, 23(7): 34
[13] 汤喜兰, 刘建勋, 李磊. 中药有机酸类成分的药理作用及在心血管疾病的应用[J].中国实验方剂学杂志, 2012, 18(5): 243
TANG XL, LIU JX, LI L. Pharmacological effects of organic acids in Chinese herbs and its application in cardiovascular diseases[J].Chin J Exp Tradit Med Form, 2012, 18(5): 243
[14] 吴晓民, 王艳红, 陈颖, 等. 加速溶剂萃取-高效液相色谱法同时测定全叶千里光中3种成分的含量[J].药物分析杂志, 2016, 36(9): 1555
WU XM, WANG YH, CHEN Y, et al. Simultaneous determination of three components in Senecio cannabifolius var. integrifolius by accelerated solvent extraction-HPLC[J].Chin J Pharm Anal, 2016, 36(9): 1555
[15] 卢李倩, 潘红波, 徐妮娜, 等. 高效液相色谱法测定千里光药材中的绿原酸和金丝桃苷含量[J].中国处方药, 2019, 17(1): 42
LU LQ, PAN HB, XU NN, et al. The determination of chlorogenic acid and hyperoside in Senecio by HPLC[J].J China Prescr Drug, 2019, 17(1): 42