目的:建立同时测定千里光中绿原酸、隐绿原酸、咖啡酸、芦丁、金丝桃苷、异槲皮苷、异绿原酸A、槲皮苷、异绿原酸C和槲皮素10个成分含量的HPLC方法。方法:采用YMC C18色谱柱(250 mm×4.6 mm,5 μm),以乙腈(A)-0.1%磷酸水溶液(B)为流动相,梯度洗脱,流速1.0 mL·min-1,检测波长350 nm,柱温30 ℃。结果:10个成分分别在各自相应浓度范围内与峰面积呈良好的线性关系(r≥0.999 8);精密度(RSD≤0.8%)、重复性(RSD≤1.7%)、稳定性(RSD≤1.6%)良好;平均回收率(n=6)为96.0%～100.6%,RSD为0.54%～1.8%;5批样品中绿原酸、隐绿原酸、咖啡酸、芦丁、金丝桃苷、异槲皮苷、异绿原酸A、槲皮苷、异绿原酸C和槲皮素含量范围分别为1.097～3.390、0.323～0.758、0.140～0.249、0.176～0.489、0.381～0.797、0.107～0.199、0.280～0.598、0.077～0.659、0.090～0.152和0.028～0.058 mg·g-1。结论:该方法简便易行,重复性好,可为千里光药材的质量控制指标提供一定的参考。
Objective: To establish an HPLC method for the simultaneous determination of ten chemical compositions including chlorogenic acid, cryptochlorogenic acid, caffeic acid, rutin, hyperoside, isoquercitrin, isochlorogenic acid A, quercitrin, isochlorogenic acid C, and quercetin in the aerial parts of Senecio scandens. Methods: The YMC C18 column(250 mm×4.6 mm, 5 μm) was used, the mobile phase was acetonitrile (A) -0.2% phosphate aqueous(B) with gradient elution, at the flow rate of 1.0 mL·min-1, the detection wavelength was 350 nm, and the column temperature was at 30 ℃. Results: The linear relationship of the ten chemical components was good(r ≥0.999 8). Results of precision(RSD≤0.8%), repeatability(RSD≤1.7%) and stability (RSD≤1.6%) were good. The average recoveries (n=6) were 96.0%-100.6% with RSDs of 0.54%-1.8%. The contents of chlorogenic acid, cryptochlorogenic acid, caffeic acid, rutin, hyperoside, isoquercitrin, isochlorogenic acid A, quercitrin, isochlorogenic acid C, and quercetin in five batches of samples were 1.097-3.390、0.323-0.758、0.140-0.249、0.176-0.489、0.381-0.797、0.107-0.199、0.280-0.598、0.077-0.659、0.090-0.152 and 0.028-0.058 mg·g-1, respectively. Conclusion: This method is specific, accurate and reproducible, and it can provide reference for the quality control of Senecio scandens.
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