Objective: To establish a method for simultaneous determination of fingerprint and multi-target ingredients and quality control and evaluation in Swertia franchetiana H.Smith by HPLC. Methods: The analysis was performed on Waters Sunfire ODS-C18 column with mobile phase consisting of methanol -0.05% phosphoric acid solution with gradient elution at 1.0 mL·min-1. The detection wavelength was 254 nm and the column temperature was 30 ℃. Samilarity evaluation combined with Chempattern chemical software was used to evaluate the fingerprint established by HPLC. The content of 10 index components were determined by HPLC. Results: Swertia franchetiana H.Smith fingerprint was established, similarity＞0.904, 29 common peaks were confirmed. 10 chromatographic peaks of loganin, swertiamarin, gentiopicroside, sweroside, mangiferin, homoorientin, amarogentin, mercaptosid, 1,8-dihydroxy-3,7-dimethoxyxanketo, bellidifolin were identified,ten components showed good linear relationships(r≥0.999 3). The recoveries were 96.8%-99.3%, RSD<2.1%. The average content determination result were 0.16%, 3.63%, 0.23%, 0.38%, 0.24%, 0.26%, 0.07%, 1.13%, 0.08%, 0.25%. The results showed that the content of swertiamarin and mercaptosid was high. Conclusion: The HPLC fingerprint combined with the content determination method of multi index components is suitable for the quality control and evaluation of Swertia franchetiana H.Smith. This method is fast, simple, stable and reliable, and can provide a basis for establishing the quality standard of Tibetan medicine Swertia franchetiana H.Smith.
[1] 中国科学院中国植物志编辑委员会. 中国植物志. 第2卷[M]. 北京: 科学出版社, 1988: 402
Editorial Committee of Flora of China. Chinese Academy of Sciences. Flora of China. Vol 2[M]. Beijing: Science Press, 1988: 402
[2] 中国科学院西北高原生物研究所. 青海植物志. 第3卷[M]. 西宁: 青海人民出版社, 1991: 95
Northwest Plateau Biology Institute. Chinese Academy of Sciences. Flora of the Sea. Vol 3[M]. Xining: Qinghai People’s Publishing House, 1991: 95
[3] 中国科学院青藏高原综合科学考察队. 西藏植物志. 第3卷[M]. 北京: 科学出版社, 1986: 994
Qinghai Tibet Plateau Comprehensive Scientific Expedition of Chinese Academy of Sciences. Flora of Tibet. Vol 3[M]. Beijing: Science Press, 1986: 994
[4] 中国科学院西北高原生物研究所. 青海经济植物志[M]. 西宁: 青海人民出版社, 1987: 461
Northwest Plateau Biology Institute. Chinese Academy of Sciences. Economic Flora of Qinghai [M]. Xining: Qinghai People’s Publishing House, 1987: 461
[5] 中国科学院西北高原生物研究所. 藏药志[M]. 西宁: 青海人民出版社, 1991: 110
Northwest Plateau Institute of Biology, Chinese Academy of Sciences. Records of Tibetan Medicine [M]. Xining: Qinghai People’s Publishing House, 1991: 110
[6] 文检, 张艺, 刘川, 等. 藏药“蒂达”的基原、 药性及功效的考证[J].中国中药杂志, 2016, 41(4): 609
WEN J, ZHANG Y, LIU C, et al. Research on the origin, properties and efficacy of Tibetan medicine “Dida”[J].China J Tradit Chin Mater Med, 2016, 41(4): 609
[7] 钟国跃, 王昌华, 刘翔, 等. 常用藏药“蒂达(藏茵陈)”的资源与使用现状调查[J].世界科学技术—中医药现代化, 2010, 12(1): 122
ZHONG GY, WANG CH, LIU X, et al. Survey on resources and use status of commonly used Tibetan medicine “Dida (Zang Yin Cheng)”[J].World Sci Technol Mod Tradit Chin Med, 2010, 12(1): 122
[8] 钟国跃, 阳勇, 冯婷婷, 等. 常用藏药“蒂达”(藏茵陈)基原物种药用合理性及资源利用价值评价[J].中国中药杂志, 2012, 37(17): 2639
ZHONG GY, YANG Y, FENG TT, et al. Evaluation of medicinal reasonableness and resource utilization of Dida (Zang Yin Cheng) [J].China J Tradit Chin Mater Med, 2012, 37(17): 2639
[9] 纪兰菊, 保怡, 陈桂琛, 等. 15种獐牙菜属植物中主要药用成分的高效液相色谱测定[J].西北植物学报, 2004, 24(7): 1298
JI LJ, BAO Y, CHEN GC, et al. Determination of main medicinal components in 15 species of Swertia by HPLC[J].Acta Bot Sin, 2004, 24(7): 1298
[10] 马玉花, 纪兰菊, 陈桂琛, 等. 抱茎獐牙菜中3种药效成分的HPLC测定[J].安徽农业科学, 2009, 37(4): 1623
MA YH, JI LJ, CHEN GC, et al. HPLC determination of three effective constituents in Swertia davidii [J].Anhui Agric Sci, 2009, 37(4): 1623
[11] 张喜民, 李敏, 邓英, 等. 抱茎獐牙菜正丁醇提取部位对肝损伤保护及免疫调节作用的研究[J].中成药, 2011, 33(1): 139
ZHANG XM, LI M, DENG Y, et al. Study on the protective and immunomodulatory effects of n-butanol extract from Swertia ampullata on liver injury [J].Chin Tradit Pat Med, 2011, 33(1): 139
[12] 马玉花, 纪兰菊, 陈桂琛, 等. 抱茎獐牙菜中当药黄素的 HPLC 测定研究[J].安徽农业科学, 2008, 36(8): 3248
MA YH, JI LJ, CHEN GC, et al. Study on HPLC determination of the active flavin in Swertia ampullata[J].Anhui Agric Sci, 2008, 36 (8): 3248
[13] 王世盛, 徐青, 肖红斌, 等. 抱茎獐牙菜中的苷类成分[J].中草药,2004, 35(8): 847
WANG SS, XU Q, XIAO HB, et al. Glycosides from Swertia armeniaca [J].Chin Tradit Herb Drugs, 2004, 35(8): 847
[14] 马玉花, 陈桂琛,纪兰菊, 等. 不同部位抱茎獐牙菜中当药醇苷的 HPLC 测定[J].青海科技, 2008, 15(6): 25
MA YH, CHEN GC, JI LJ, et al. HPLC determination of the active glycosides in Swertia armeniaca [J].Qinghai Sci Technol, 2008, 15(6): 25
[15] 张杰, 王志平, 唐荣江, 等. 藏药抱茎樟牙菜药理实验研究[J].西北药学杂志, 1988, 3(4): 15
ZHANG J, WANG ZP, TANG RJ, et al. Pharmacological experimental study on Tibetan medicine Cinnamomum cassia[J].Northwest Pharm J, 1988, 3(4): 15
[16] 朱丹丹, 杨建华, 居博伟, 等. 新疆假龙胆不同部位中3种环烯醚萜类成分的含量比较研究[J].西北药学杂志, 2018, 33(2): 171
ZHU DD, YANG JH, JU BW, et al. A comparative study on the contents of three iridoids in different parts of pseudogentian from Xinjiang [J].Northwest Pharm J, 2018, 33(2): 171
[17] 青海省藏药标准[S]. 1992: 5
Tibetan Medicine Standard of Qinghai Province[S]. 1992: 5
[18] 李立. 人工栽培藏茵陈类药用植物获得成功[J].河北中医, 2003, 25(2): 156
LI L. The success of artificial cultivation of Tibetan medicinal plants[J].Hebei J Tradit Chin Med, 2003, 25(2): 156
[19] 陈桂琛. 藏药抱茎獐牙菜的引种栽培研究[J].中国中药杂志, 2005, 30(12): 1957
CHEN GC. Study on the introduction and cultivation of Swertia armeniaca [J].China J Tradit Chin Med, 2005, 30(12): 1957
[20] 张幸福, 骆桂法, 王燕. HPLC同时测定抱茎獐牙菜中獐牙菜苦苷、龙胆苦苷和芒果苷含量[J].实验方剂学杂志, 2014, 20(12): 61
ZHANG XF, LUO GF, WANG Y. Simultaneous determination of swertiamarin, gentiopicrin and mangiferin in Swertia franchetiana H.Smith by HPLC[J].Chin J Exp Tradit Med Form, 2014, 20(12): 61
[21] 纪兰菊, 保怡, 马玉花, 等. 藏药抱茎獐牙菜HPLC指纹图谱研究[J].西北植物学报, 2004, 24(11): 2092
JI LJ, BAO Y, MA YH, et al. Study on the chromatographic fingerprint of Tibet herb Swertia franchetiana H.Smith by HPLC[J].Northwest J Bot, 2004, 24 (11): 2092
[22] 周剑波, 蒋福全, 赵晓辉. 抱茎獐牙菜提取物的 HPLC指纹图谱研究[J].中成药, 2007, 29(4): 473
ZHOU JB, JIANG FQ, ZHAO XH. Study on the chromatographic fingerprint of Tibetan medicine Swertia franchetiana H.Smith extract by HPLC [J].Chin Tradit Pat Med, 2007, 29(4): 473
