目的: 利用色差原理量化土茯苓断面不同颜色,分析其断面颜色与成分含量的相关性。方法: 运用nh310高品质电脑色差仪测定土茯苓断面颜色值L*、a*、b*、Eab*,测定土茯苓中浸出物的含量,采用高效液相色谱法测定土茯苓中落新妇苷、黄杞苷的含量;采用SPSS 25.0、SIMCA 13.0对数据进行分析。结果: 颜色值能够很好地代表实际药材颜色,通过聚类分析、偏最小二乘法分析以及相关性分析显示,浸出物及成分含量分别与颜色值a*、b*呈极显著正相关(P<0.01),与颜色值L*、Eab*呈极显著负相关(P<0.01)。结论: 土茯苓浸出物及落新妇苷、黄杞苷含量与其不同断面颜色之间存在极显著相关性,2种断面颜色之间成分含量的差异主要表现为断面淡红棕色者较淡白色者含量较高,为土茯苓药材质量提供新思路。
Objective: To investigate the difference of contents of extract, astilbin and engeletin among different cross sections color of Smilacis Glabrae Rhizoma, and to analyze the correlation between color and component content. Methods: 26 Batches of Smilacis Glabrae Rhizoma with different cross sections were collected, and their color was observed by naked eye and numbered according to light reddish brown and off-white cross sections respectively. The color value of Smilacis Glabrae Rhizoma powder was determined by color difference meter, its moisture was determined by oven drying method, the content of extract was determined by alcohol hot soaking method, and the contents of asxinobin and flavarin were determined by HPLC. Cluster analysis, partial least square method and correlation analysis were used to sort out and analyze the data. Results: The color of the chromatic meter measuring value well represented the actual medicinal material color. Partial least squares analysis results showed that the color value a* was the main parameters affecting the classification. Correlation analysis showed that the contents of extract, astilbin and engeletin were significantly positively correlated with color values a* and b* (P<0.01), and significantly negatively correlated with color values L* and Eab* (P<0.01). Conclusion: There is a very significant correlation between the content of the extract astirin and engeletin of Smilacis Glabrae Rhizoma, and the color of different sections. The difference in the content of components between the two section colors is mainly reflected in the higher content of the light red-brown section than the light white section, which provides a new way for the quality of Smilacis Glabrae Rhizoma.
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